1887

Abstract

The rate of oxygen uptake by single macroplasmodia of increased in two steps during each synchronous mitotic cycle. Plateaux in the respiratory pattern, of 0·22 cycles duration, occurred in mid-interphase and in the period up to and including mitosis. A fall in the rate of respiration was frequently associated with mitosis itself. This pattern of respiration continued for more than 10 mitotic cycles after inoculation and was unaffected by omission of an initial routine period of starvation. A similar stepped pattern of respiration was observed in synchronously germinating spherules during the period between outgrowth and the first mitosis. The specific activities of succinate dehydrogenase, fumarase and malate dehydrogenase remained relatively constant during the mitotic cycle, while fluctuations in cytochrome oxidase activity paralleled those in specific respiratory activity. Possible mechanisms for controlling the pattern of respiration are discussed with reference to published data on protoplasmic streaming and ATP concentrations during the mitotic cycle.

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1979-11-01
2021-10-23
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References

  1. Barath Z., Küntzel H. 1972; Cooperation of mitochondrial and nuclear genes specifying the mitochondrial genetic apparatus in Neurospora crassa . Proceedings of the National Academy of Sciences of the United States of America 69:1371–1374
    [Google Scholar]
  2. Bersier D., Braun R. 1974; Pools of deoxyribo-nucleoside triphosphates in the mitotic cycle of Physarum . Biochimica et biophysica acta 340:463–471
    [Google Scholar]
  3. Birch B., Turnock G. 1977; Synthesis of protein during the nuclear division cycle in Physarum polycephalum . FEBS Letters 84:317–319
    [Google Scholar]
  4. Braun R., Evans T. E. 1969; Replication of nuclear satellite and mitochondrial DNA in the mitotic cycle of Physarum polycephalum . Biochimica et biophysica acta 182:511–522
    [Google Scholar]
  5. Breidenbach R. W., Castelfranco P., Criddle R. S. 1967; Biogenesis of mitochondria in germinating peanut cotyledons. II. Changes in cytochromes and mitochondrial DNA. Plant Physiology 42:1025–1041
    [Google Scholar]
  6. Brewer E. N. 1972; Polysome profiles, amino acid incorporation in vitro, and polysome reaggregation following disaggregation by heat and shock in Physarum polycephalum . Biochimica et biophysica acta 277:639–645
    [Google Scholar]
  7. Chin B., Bernstein I. A. 1968; Adenosine triphosphate and synchronous mitosis in Physarum polycephalum . Journal of Bacteriology 108:1072–1086
    [Google Scholar]
  8. Cottrell S. F., Avers C. J. 1970; Evidence of mitochondrial synchrony in synchronous cell cultures of yeast. Biochemical and Biophysical Research Communications 38:973–980
    [Google Scholar]
  9. Cottrell S. F., Rabinowitz M., Getz G. S. 1975; Cytochrome synthesis in synchronous cultures of the yeast, Saccharomyces cerevisiae . Journal of Biological Chemistry 250:4087–4094
    [Google Scholar]
  10. Daniel J. W., Baldwin H. H. 1964; Methods of culture for plasmodial myxomycetes. Methods in Cell Physiology 1:9–41
    [Google Scholar]
  11. Dharmalingam K., Jayaraman J. 1973; Mitochondriogenesis in synchronous cultures of yeast. 1. Oscillatory pattern of respiration. Archives of Biochemistry and Biophysics 157:197–202
    [Google Scholar]
  12. Fink K. 1975; Fluctuations in the deoxy- and ribonucleoside triphosphate pools during the mitotic cycle of Physarum polycephalum . Biochimica et biophysica acta 414:85–89
    [Google Scholar]
  13. Forde B. G., John P. C. L. 1973; Stepwise accumulation of autoregulated enzyme activities during the cell cycle of the eucaryote Chlorella . Experimental Cell Research 19:127–135
    [Google Scholar]
  14. Greksák M., Hanicová M. 1973; Activities of succinate dehydrogenase and cytochrome oxidase in synchronous cultures of aerobically and anaerobically grown yeast. Biologica, Bratislava 28:425–433
    [Google Scholar]
  15. Guttes E., Guttes S., Rusch H. P. 1961; The morphology and development of Physarum polycephalum grown in pure culture. Developmental Biology 3:588–614
    [Google Scholar]
  16. Guttes E., Guttes S., Devi V. R. 1969; Division stages of the mitochondria in normal and actinomycin D-treated plasmodia of Physarum polycephalum . Experientia 25:66–68
    [Google Scholar]
  17. Hlall L., Turnock G. 1976; Synthesis of ribosomal RNA during the mitotic cycle in the slime mould Physarum polycephalum . European Journal of Biochemistry 62:471–477
    [Google Scholar]
  18. Hüttermann A., Porter M. T., Rusch H. P. 1970; Activity of some enzymes in Physarum polycephalum. 1. In the growing plasmodium. Archiv für Mikrobiologie 74:90–100
    [Google Scholar]
  19. Kuenzi M. T., Fietcher A. 1969; Changes in carbohydrate composition during the budding cycle of Saccharomyces cerevisiae . Archiv für Mikrobiologie 64:396–407
    [Google Scholar]
  20. Lloyd D., Turner G., Poole R. K., Nicholl W. G., Roach G. I. 1971; A hypothesis of nuclear-mitochondrial interactions based on ex-periments with Tetrahymena pyriformis . Subcellular Biochemistry 1:91–95
    [Google Scholar]
  21. Mitchison J. M. 1971 The Biology of the Cell Cycle Cambridge University Press;
    [Google Scholar]
  22. Mitchison J. M. 1977; Enzyme synthesis during the cell cycle. In Cell Differentiation in Micro-organisms, Plants and Animals pp. 377–401 Nover L., Mothes K. Edited by Jena: Fischer;
    [Google Scholar]
  23. Mittermayer C., Braun R., Rusch H. P. 1964; RNA synthesis in the mitotic cycle of Physarum polycephalum . Biochimica et biophysica acta 91:399–405
    [Google Scholar]
  24. Mittermayer C., Braun R., Chayka T. C., Rusch H. P. 1966; Polysome patterns and protein synthesis during the mitotic cycle of Physarum polycephalum . Nature; London: 2101133–1137
    [Google Scholar]
  25. Nosoh Y., Takamiya A. 1962; Synchronisation of the budding cycle in yeast cells, and the effect of carbon monoxide and nitrogen deficiency on the synchrony. Plant and Cell Physiology 3:53–66
    [Google Scholar]
  26. Nygaard O. F., Guttes S., Rusch H. P. 1960; Nucleic acid metabolism in a slime mould with synchronous mitosis. Biochimica et biophysica acta 38:298–306
    [Google Scholar]
  27. Poole R. K. 1977; The influence of growth sub-strate and capacity for oxidative phosphorylation on respiratory oscillations in synchronous cultures of Escherichia coli K12. Journal of General Microbiology 99:369–377
    [Google Scholar]
  28. Poole R. K., Lloyd D. 1974; Changes in respiratory activities during the cell cycle of the fission yeast Schizosaccharomyces pombe 972h growing in the presence of glycerol. Biochemical Journal 144:141–148
    [Google Scholar]
  29. Poole R. K., Lloyd D., Kemp R. B. 1973; Respiratory oscillations and heat evolution in synchronously dividing cultures of the fission yeast Schizosaccharomyces pombe 972h . Journal of General Microbiology 77:209–220
    [Google Scholar]
  30. Racker E. 1950; Spectrophotometric measurement of fumaric and cis-aconitic acids. Biochimica et biophysica acta 4:211–214
    [Google Scholar]
  31. Robbins E., Morrill G. A. 1969; Oxygen uptake during the HeLa cell life cycle and its correlation with macromolecular synthesis. Journal of Cell Biology 43:629–633
    [Google Scholar]
  32. Sachsenmaier W. 1964; Zur DNS- und RNS-synthese in Teilungszyklus synchroner Plasmodien von Physarum polycephalum . Biochemische Zeitschrift 340:541–547
    [Google Scholar]
  33. Sachsenmaier W., Ives D. H. 1965; Periodische Änderungen der Thymidinkinase Activität in synchronen Mitosecyclus von Physarum polycephalum . Biochemische Zeitschrift 343:399–406
    [Google Scholar]
  34. Sachsenmaier W., Immich H., Grunst J., Scholz R., Bücher TH. 1969; Free ribonucleotides of Physarum polycephalum . European Journal of Biochemistry 8:557–561
    [Google Scholar]
  35. Sachsenmaier W., Blessing J., Brauser B., Hansen K. 1973; Protoplasmic streaming in Physarum polycephalum: observations of spontaneous and induced changes of the oscillatory pattern by photometric and fluorometric techniques. Protoplasma 77:381–396
    [Google Scholar]
  36. Schatz G., Mason T. 1974; The biosynthesis of mitochondrial proteins. Annual Review of Biochemistry 43:51–87
    [Google Scholar]
  37. Scopes A. W., Williamson D. H. 1964; The growth and oxygen uptake of synchronously dividing cultures of yeast. Biochemical and Biophysical Research Communications 38:750–757
    [Google Scholar]
  38. Singer T. P., Kearney E. B., Kenney W. C. 1973; Succinate dehydrogenase. Advances in Enzymology 37:189–272
    [Google Scholar]
  39. Smith L. 1955; Spectrophotometric assay of cytochrome oxidase. Methods of Biochemical Analysis 2:427–434
    [Google Scholar]
  40. Teague W. M., Henney H. R. Jr 1973; Purification and properties of cytoplasmic and mitochondrial malate dehydrogenases of Physarum polycephalum . Journal of Bacteriology 116:673–684
    [Google Scholar]
  41. Wiemken A., Matile P., Moor H. 1970; Vacuolar dynamics in synchronously budding yeast. Archiv für Mikrobiologie 70:89–103
    [Google Scholar]
  42. Wolf H., Finkenstedt G., Woertz G., Sachsenmaier W. 1973; Regulation von Nucleosid-phosphorylierenden Enzymen im synchronen Mitose-zyklus vonPhysarum polycephalum . Hoppe-Seyler’s Zeitschrift für physiologische Chemie 354:1260
    [Google Scholar]
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