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Di- and tripeptides shared the same transport system in Saccharomyces cerevisiae. Typical rates of uptake were 1 to 5 nmol peptide min−1 (mg dry wt)−1; these rates were influenced by the composition and sequence of the peptide. Accumulation of intact sarcosine peptides was demonstrated. Rates of peptide uptake were essentially the same in the wild-type and in several amino acid permease-deficient mutants. These results were derived from application of two fluorescence methods that permit direct determination of peptide uptake.
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