1887

Abstract

subsp. (PG1 and strain Y) proteins were solubilized in Triton X-100, and the antigenic proteins were precipitated from this complex mixture by addition of antiserum and then separated by two-dimensional gel electrophoresis. Of the 300 proteins solubilized, about 10 were precipitated. Proteins of PG1, a slow-growing, small colony (SC) strain, were precipitated by antiserum to PG1 and by antiserum to strain Y, a fast-growing, large colony (LC) strain. Similarly, strain Y proteins were precipitated by antiserum to PG1 and by antiserum to strain Y. The few proteins precipitated in this way gave similar patterns after two-dimensional gel electrophoresis indicating that many of the dominant protein antigens of PG1 and strain Y are shared. Antiserum to subsp. (PG3) also precipitated some proteins of strain Y. Antiserum to gave no reaction with any antigens. It was concluded that, in addition to the polysaccharide antigens, there are proteins in that are antigenic and that some of these are found in both the SC and LC strains of subsp. and also in subsp.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-115-1-111
1979-11-01
2021-07-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/115/1/mic-115-1-111.html?itemId=/content/journal/micro/10.1099/00221287-115-1-111&mimeType=html&fmt=ahah

References

  1. Al-Aubaidi J. M., Dardiri A. H., Fabricant J. 1972; Biochemical characterization and antigenic relationship of Mycoplasma mycoides subsp. mycoides Freundt and Mycoplasma mycoides subsp. capri (Edward) Freundt. International Journal of Systematic Bacteriology 22:155–164
    [Google Scholar]
  2. Archer D. B., Rodwell A. W., Rodwell E. S. 1978; The nature and location of Acholeplasma laidlawii membrane proteins investigated by twodimensional gel electrophoresis. Biochimica et biophysica acta 513:268–283
    [Google Scholar]
  3. Askaa G., Ernø H., Ojo M. O. 1978; Bovine mycoplasmas: classification of groups related to Mycoplasma mycoides . Acta veterinaria scandinavica 19:166–178
    [Google Scholar]
  4. Buttery S. H. 1970; Hapten inhibition of the reaction between Mycoplasma mycoides polysaccharide and bovine antisera. Immunochemistry 7:305–310
    [Google Scholar]
  5. Buttery S. H., Plackett P. 1960; A specific polysaccharide from Mycoplasma mycoides . Journal of General Microbiology 23:357–368
    [Google Scholar]
  6. Cottew G. S., Yeats F. R. 1978; Subdivision of Mycoplasma mycoides subsp. mycoides from cattle and goats into two types. Australian Veterinary Journal 54:293–296
    [Google Scholar]
  7. Ernø H., Jurmanová K. 1973; Bovine mycoplasmas: serological studies by double immunodiffusion, growth precipitation and growth inhibition. Acta veterinaria scandinavica 14:524–537
    [Google Scholar]
  8. Ghangas G. S., Milman G. 1977; Hypoxanthine phosphoribosyltransferase: two-dimensional gels from normal and Lesch-Nyhan hemolyzates. Science 196:1119–1120
    [Google Scholar]
  9. Herderscheê D. 1963; An improved medium for the cultivation of the Eaton agent. Antonie van Leeuwenhoek 29:154–156
    [Google Scholar]
  10. Hudson J. R., Cottew G. S., Adler H. E. 1967; Diseases of goats caused by mycoplasma: a review of the subject with some new findings. Annals of the New York Academy of Sciences 143:287–297
    [Google Scholar]
  11. Johansson K. -E., Hjertén S. 1974; Localization of the Tween 20-soluble membrane proteins of Acholeplasma laidlawii by crossed immuno electrophoresis. Journal of Molecular Biology 86:341–348
    [Google Scholar]
  12. Jones P. P. 1977; Analysis of H-2 and la molecules by two-dimensional gel electrophoresis. Journal of Experimental Medicine 146:1261–1279
    [Google Scholar]
  13. Jones A. S., Tittensor J. R., Walker R. T. 1965; The chemical composition of the nucleic acids and other macromolecular constituents of Mycoplasma mycoides var.capri . Journal of General Microbiology 40:405–411
    [Google Scholar]
  14. Kahane I., Razin S. 1969; Immunological analysis of mycoplasma membranes. Journal of Bacteriology 100:187–194
    [Google Scholar]
  15. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  16. Laws L. 1956; A pleuropneumonia-like organism causing peritonitis in goats. Australian Veterinary Journal 32:326–329
    [Google Scholar]
  17. Ne’eman Z., Kahane I., Kovartovsky J., Razin S. 1972; Characterization of the mycoplasma membrane proteins. III. Gel filtration and immunological characterization of Acholeplasma laidlawii membrane proteins. Biochimica et biophysica acta 266:255–268
    [Google Scholar]
  18. Razin S. 1968; Mycoplasma taxonomy studied by electrophoresis of cell proteins. Journal of Bacteriology 96:687–694
    [Google Scholar]
  19. Razin S., Kahane I., Kovartovsky J. 1972; Immunochemistry of mycoplasma membranes. In Pathogenic Mycoplasmas pp. 93–122 A Ciba Foundation Symposium. Amsterdam: Associated Scientific Publishers;
    [Google Scholar]
  20. Rodwell A. W., Rodwell E. S. 1978; Phylogenetic relationships between strains of Mycoplasma mycoides subspp. mycoides and capri studied by two-dimensional gel electrophoresis of cell proteins. Journal of General Microbiology 109:259–263
    [Google Scholar]
  21. Rodwell A. W., Peterson J. E., Rodwell E. S. 1972; Macromolecular synthesis and growth of mycoplasmas. In Pathogenic Mycoplasmas pp. 123–144 A Ciba Foundation Symposium. Amsterdam: Associated Scientific Publishers;
    [Google Scholar]
  22. Rodwell A. W., Peterson J. E., Rodwell E. S. 1973; Nature of striated structures in mycoplasmas. Annals of the New York Academy of Sciences 225:190–200
    [Google Scholar]
  23. Rodwell A. W., Peterson J. E., Rodwell E. S. 1975; Striated fibers of the rho form of Mycoplasma: in vitro reassembly, composition and structure. Journal of Bacteriology 122:1216–1229
    [Google Scholar]
  24. Thirkill C. E., Kenny G. E. 1974; Serological comparison of five arginine-utilizing Mycoplasma species by two-dimensional immunoelectrophoresis. Infection and Immunity 10:624–632
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-115-1-111
Loading
/content/journal/micro/10.1099/00221287-115-1-111
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error