1887

Abstract

Nine independent mutants deficient in tryptophanase activity were isolated. Each mutation was transferred to a specialized transducing phage that carries the tryptophanase region of the chromosome. The nine phages thus produced, and a tenth carrying a previously characterized tryptophanase mutation, were used to lysogenize a bacterial strain harbouring a mutation in the tryptophanase structural gene and also a suppressor of polarity. In no case was complementation observed; we conclude that there is no closely linked positive regulatory gene for tryptophanase.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-114-2-471
1979-10-01
2021-05-11
Loading full text...

Full text loading...

/deliver/fulltext/micro/114/2/mic-114-2-471.html?itemId=/content/journal/micro/10.1099/00221287-114-2-471&mimeType=html&fmt=ahah

References

  1. Bloom F.R., McFall E. 1975; Isolation and characterization of d-serine deaminase constitutive mutants by utilization of d-serine as sole carbon or nitrogen source. Journal of Bacteriology 121:1078–1084
    [Google Scholar]
  2. Bloom F.R., McFall E., Young M.C., Carothers A.M. 1975; Positive control in the d-serine deaminase system of Escherichia coli K-12. Journal of Bacteriology 121:1092–1101
    [Google Scholar]
  3. Borck K., Beggs J.D., Brammar W.J., Hopkins A.S., Murray N.E. 1976; The construction in vitro of transducing derivatives of phage lambda. Molecular and General Genetics 146:199–207
    [Google Scholar]
  4. Gilbert W., MÜller-Hill B. 1970; The lactose repressor. In The Lactose Operon ch. 5. Beckwith J.R., Zipser D. Edited by Cold Spring Harbor: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  5. Hong J.-S., Ames B.N. 1971; Localized mutagenesis of any specific small region of the bacterial chromosome. Proceedings of the National A cademy of Sciences of the United States of America 68:3158–3162
    [Google Scholar]
  6. Korn L.J., Yanofsky C. 1976; Polarity suppressors increase expression of the wild-type tryptophan operon of Escherichia coli. Journal of Molecular Biology 103:395–409
    [Google Scholar]
  7. London J., Goldberg M.E. 1972; The tryptophanase from Escherichia coli K-12.I.Purification, physical properties and quaternary structure. Journal of Biological Chemistry 247:1566–1570
    [Google Scholar]
  8. Taylor H.V., Yudkin M.D. 1978; Synthesis of tryptophanase in Escherichia coli: isolation and characterization of a structural-gene mutant and two regulatory mutants. Molecular and General Genetics 165:95–102
    [Google Scholar]
  9. Tully M.J., Yudkin M.D. 1977; Fine-structure mapping and complementation analysis of the Escherichia coli cysB gene. Journal of Bacteriology 131:49–56
    [Google Scholar]
  10. Wilcox G., Clemetson K.J., Cleary P., Englesberg E. 1974; Interaction of the regulatory gene product with the operator site in the l-arabinose operon of Escherichia coli. Journal of Molecular Biology 85:589–602
    [Google Scholar]
  11. Yudkin M.D. 1976; Mutations in Escherichia coli that relieve catabolite repression of tryptophanase synthesis.Mutations distant from the tryptophanase gene. Journal of General Microbiology 92:125–132
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-114-2-471
Loading
/content/journal/micro/10.1099/00221287-114-2-471
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error