@article{mbs:/content/journal/micro/10.1099/00221287-114-2-401, author = "Bingham, Alistair H. A. and Bruton, Chris J. and Atkinson, Tony", title = "Isolation and Partial Characterization of Four Plasmids from Antibiotic-resistant Thermophilic Bacilli", journal= "Microbiology", year = "1979", volume = "114", number = "2", pages = "401-408", doi = "https://doi.org/10.1099/00221287-114-2-401", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-114-2-401", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: Twenty-nine antibiotic-resistant isolates of thermophilic bacilli were examined for the presence of covalently closed circular duplex DNA molecules by agarose-gel electrophoresis and caesium chloride-ethidium bromide density gradient centrifugation. Five of the 29 strains tested contained covalently closed circular molecules. Two of the streptomycin-resistant strains contained the same two plasmids: pAB118A of molecular weight 4·9 × 106 (7·0 kilobases) and pAB118B of molecular weight 3·0 × 106 (4·3 kilobases). Two of the tetracycline-resistant strains each contained a plasmid (pAB124) of molecular weight 2·9 × 106 (4·14 kilobases), while a third harboured a small plasmid (pAB128) of molecular weight 2·5 × 106 (3·57 kilobases). These plasmids were digested with 19 different restriction endonucleases and the numbers of cleavage sites were determined. Transformation of Bacillus subtilis 168 (Trp−) with purified plasmid DNA indicated that pAB124 conferred tetracycline resistance on the host.", }