Studies on Recently Isolated Cultures of Methicillin-resistant Free

Abstract

Summary: Of 19 recently isolated cultures of raethicillin-resistant 18 showed inducible low-level resistance to minocycline, 15 showed high-level resistance to streptomycin, and 4 showed resistance to low levels of streptomycin. Two cultures produced yellow pigment and may have been derived by loss of a gene(s) determining orange pigment.

Treatment of three cultures with serial exposures to -methyl--nitro--nitrosoguanidine resulted in a widening of phage typing pattern that included all reactions in group I, the great majority in group 111, but none in group 11. The widening in phage lysis was possibly due to the elimination of defective prophages. Transfer of tetracycline resistance occurred from 12 out of the 19 cultures to a recipient in mixed culture; this transfer required either Ca or Mg, was abolished by citrate, and enhanced by high cell density. It was probablymediated by defective bacteriophages.

No evidence was obtained for the occurrence of recombination within the methicillinresistant clone in nature. Eleven methicillin-resistant cultures stored for at least 5 years on agar slopes at 20 °C had all lost this resistance at high frequency.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-114-2-329
1979-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/114/2/mic-114-2-329.html?itemId=/content/journal/micro/10.1099/00221287-114-2-329&mimeType=html&fmt=ahah

References

  1. Annear D. I., Grubb W. B. 1976; Methicillin sensitive variants in ageing broth cultures of methieillin-resistant Staphylococcus aureus. Pathology 8:69–72
    [Google Scholar]
  2. Asheshov E. H. 1975; The genetics of tetracycline resistance in Staphylococcus aureus. Journal of General Microbiology 88:132–140
    [Google Scholar]
  3. Asheshov E. H., Jevons M. P. 1963; The effect of heat on the ability of a host strain to support the growth of a Staphylococcus phage. Journal of General Microbiology 31:97–107
    [Google Scholar]
  4. Asheshov E. H., Rippon J. E. 1959; Changes in typing pattern of phage-type 80 staphylococci. Journal of General Microbiology 20:634–643
    [Google Scholar]
  5. Asheshov E. H., Winkler K. C. 1966; Staphylococcus aureus strains of the ‘52, 52A, 80, 81’ complex. Nature London: 209638–639
    [Google Scholar]
  6. Duval-Iflah Y., Heijenoort J., van Rousseau M., Raibaud P. 1977; Lysogenic conversion for multiple characters in a strain of Staphylococcus aureus. Journal of Bacteriology 130:1281–1291
    [Google Scholar]
  7. Grinsted J., Lacey R. W. 1973; Ecological and genetic implications of pigmentation in Staphylococcus aureus. Journal of General Microbiology 75:259–267
    [Google Scholar]
  8. Jevons M. P., Parker M. T. 1964; The evolution of new hospital strains of Staphylococcus aureus. Journal of Clinical Pathology 17:243–250
    [Google Scholar]
  9. Jevons M. P., John M., Parker M. T. 1966; Cultural characters of a newly recognised group of hospital staphylococci. Journal of Clinical Pathology 19:305–312
    [Google Scholar]
  10. Lacey R. W. 1971; Transfer of tetracycline- resistance between strains of Staphylococcus aureus in mixed cultures. Journal of General Microbiology 69:229–237
    [Google Scholar]
  11. Lacey R. W. 1972; Genetic control in methieillin- resistant strains of Staphylococcus aureus. Journal of Medical Microbiology 5:497–508
    [Google Scholar]
  12. Lacey R. W. 1975; Antibiotic resistance plasmids of Staphylococcus aureus and their clinical importance. Bacteriological Reviews 39:1–32
    [Google Scholar]
  13. Lacey R. W., Grinsted J. 1973; Genetic analysis of methieillin-resistant strains of Staphylococcus aureus; evidence for their evolution from a single clone. Journal of Medical Microbiology 6:511–526
    [Google Scholar]
  14. Oeding P. 1974; Cellular antigens of staphylococci. Annals of New York Academy of Sciences 236:15–21
    [Google Scholar]
  15. Parker M. T., Asheshov E. H., Hewitt J. H., Nakhla V. S., Brock B. M. 1974; Endemic staphylococcal infections in hospitals. Annals of New York Academy of Sciences 236:406–484
    [Google Scholar]
  16. Ralston D. J., Baer B. S. 1964; A new property of phage group II Staphylococcus aureus: host restriction of phage K14. Journal of General Microbiology 36:1–16
    [Google Scholar]
  17. Rosendal K., Bülow P. 1971; A subdivision of Staphylococcus aureus strains belonging to the 82A, 84, 85, 6557, 592 complex with special reference to antibiotic resistance. Acta pathologica et microbiologica scandinavica B79:377–384
    [Google Scholar]
  18. Rosendal K., Jessen O., Bentzon M. W., Bulow P. 1977; Antibiotic policy and spread of Staphylococcus aureus strains in Danish hospitals, 1969-1974. Acta pathologica et microbiologica scandinavica B85:143–152
    [Google Scholar]
  19. Rountree P. M. 1959; Changes in phage-typing patterns of staphylococci following lysogenization. Journal of General Microbiology 20:620–633
    [Google Scholar]
  20. Rountree P. M., Asheshov E. H. 1961; Further observations on changes in the phage-typing pattern of phage type 80/81 staphylococci. Journal of General Microbiology 26:111–122
    [Google Scholar]
  21. Stobberingh E. E., Winkler K. C. 1976; Restrictionless mutants of Staphylococcus aureus. In Staphylococci and Staphylococcal Diseases pp. 313–324 Edited by Jeljaszewicz J. Stuttgart: Gustav Fischer Verlag;
    [Google Scholar]
  22. Willis A. T., Smith J. A., O’Connor J. J. 1966; Properties of some epidemic strains of Staphylococcus aureus. Journal of Pathology and Bacteriology 92:345–358
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-114-2-329
Loading
/content/journal/micro/10.1099/00221287-114-2-329
Loading

Data & Media loading...

Most cited Most Cited RSS feed