A Simple Technique for Assaying Certain Microbial Phytotoxins and its Application to the Study of Toxins Produced by Free

Abstract

Summary: A method for detecting and assaying phytotoxins has been developed which depends on damage caused by toxins to cells in leaves of broad bean plants and the consequent production of black polyphenol oxidation products. The assay, which has the advantage of being relatively unaffected by components of complex microbial growth media, has been used to study the properties of a toxin produced by the plant-pathogenic mycoplasma The toxin is an unstable, acidic, polar compound of low molecular weight.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-114-2-323
1979-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/114/2/mic-114-2-323.html?itemId=/content/journal/micro/10.1099/00221287-114-2-323&mimeType=html&fmt=ahah

References

  1. Daniels M. J., Meddins B. M. 1974; The pathogenicity of Spiroplasma citri. Les Colloques de l’Institut National de la Santé et de la Recherche Médicale 33:195–200
    [Google Scholar]
  2. Daniels M. J., Niven C. F. 1978; Sensitivity of plants to Spiroplasma citri toxins. Sixty-eighth Annual Report, John Innes Institute p. 105
    [Google Scholar]
  3. Daniels M. J., Markham P. G., Meddins B. M., Plaskitt A. K., Townsend R., Bar-Joseph M. 1973; Axenic culture of a plant pathogenic spiroplasma. Nature London: 244:523–524
    [Google Scholar]
  4. Dawes E. A., McGill D. J., Midgley M. 1971; Analysis of fermentation products. Methods in Microbiology 6A:53–215
    [Google Scholar]
  5. Giannotti J. 1974; Cultures de mollicutes à partir de plantesatteintes de jaunisseet d’insectes vecteurs. Les Colloques de l’Institut National de la Santé et de la Recherche Médicale 33:99–106
    [Google Scholar]
  6. Hawthorne J. D., Vandemark P. J. 1977; Metabolic studies of Spiroplasma citri. Abstracts of the Annual Meeting of the American Society for Microbiology133
    [Google Scholar]
  7. Kenten R. H. 1958; Latent phenolase in extracts of broad bean (Viciafaba L.). Biochemical Journal 68:244–251
    [Google Scholar]
  8. Markham P. G., Townsend R. 1974; Transmission of Spiroplasma citri to plants. Les Colloques de l’Institut National de la Santé et de la Recherche Médicale 33:201–206
    [Google Scholar]
  9. Markham P. G., Townsend R., Bar-Joseph M., Daniels M. J., Plaskitt A., Meddins B. M. 1974; Spiroplasmas are the causal agents of citrus little-leaf disease. Annals of Applied Biology 78:49–57
    [Google Scholar]
  10. Markham P. G., Townsend R., Plaskitt K., Saglio P. 1977; Transmission of corn stunt to dicotyledonous plants. Plant Disease Reporter 61:342–345
    [Google Scholar]
  11. Rana G. L., Kaloostian G. H., Oldfield G. N., Granett A. L., Calavan E. C., Pierce H. D., Lee I. M., Gumpf D. J. 1975; Acquisition of Spiroplasma citri through membranes by homopterous insects. Phytopathology 65:1143–1145
    [Google Scholar]
  12. Williamson D. L., Whitcomb R. F. 1975; Plant mycoplasmas: a cultivable spiroplasma causes corn stunt disease. Science 188:1018–1020
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-114-2-323
Loading
/content/journal/micro/10.1099/00221287-114-2-323
Loading

Data & Media loading...

Most cited Most Cited RSS feed