Summary: While searching for true lysogens among oxytetracycline-producing strains, free phage particles were detected and isolated from a liquid culture of ATCC 10970 (R7). The actinophage, designated RP2, appears to be a typical temperate DNA phage producing turbid plaques on the sensitive strain R6. Electron microscopic examination of RP2 lysates showed that it belongs to group B of Bradley's morphological classification. The rate of RP2 adsorption at 28 C appeared to be low. The length of the latent period was about 6h and the average burst size about 120 phage particles.


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