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Volume 114, Issue 2

Methionine Transport in : Evidence for at Least One Low-affinity Transport System Free

Abstract

Summary: The systems which transport methionine in LT2 have been studied. Fourteen mutants, isolated by three different selection procedures, had similar growth characteristics and defects in the specific transport process showing a of 0·3 for -methionine, and therefore lack the high-affinity, transport system. The sites of mutation in four of the mutants were shown by P1-mediated transduction to be linked (0·3 to 1·1 %) with a proline marker located at unit 7 on the chromosome. The high-affinity system was subject to both repression and transinhibition by methionine, and it may also be regulated by the and genes. There appeared to be at least two additional transport systems with relatively low affinities for methionine in the mutant strain, with apparent values for methionine of 24 and approximately 1·8 m. The latter system, with a very low affinity for methionine, was inhibited by leucine. In addition, methionine inhibited leucine transport, suggesting that one of the low-affinity methionine transport systems may actually be a leucine transport system.

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© Society for General Microbiology 1979
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1979-10-01
2023-12-07
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References

  1. Ames G.F. 1964; Uptake of amino acids by Salmonella typhimurium. Archives of Biochemistry and Biophysics 104:1–18
     [Google Scholar]
  2. Ames G.F., Roth J.R. 1968; Histidine and aromatic permeases of Salmonella typhimurium. Journal of Bacteriology 96:1742–1749
     [Google Scholar]
  3. Ayling P.D. 1977; Low-affinity methionine transport systems in Salmonella typhimurium. Proceedings of the Society for General Microbiology 524
     [Google Scholar]
  4. Ayling P.D., Bridgeland E.S. 1972; Methionine transport in wild-type and transportdefective mutants of Salmonella typhimurium. Journal of General Microbiology 73:127–141
     [Google Scholar]
  5. Ayling P.D., Chater K.F. 1968; The sequence of four structural and two regulatory methionine genes in the Salmonella typhimurium linkage map. Genetical Research 12:341–354
     [Google Scholar]
  6. Betteridge P.R., Ayling P.D. 1975; The role of methionine transport-defective mutations in resistance to methionine sulphoximine in Salmonella typhimurium. Molecular and General Genetics 138:41–52
     [Google Scholar]
  7. Chater K.F. 1970; Dominance of the wild-type alleles of methionine regulatory genes in Salmonella typhimurium. Journal of General Microbiology 63:95–109
     [Google Scholar]
  8. Cooper S. 1966; Utilization of d-methionine by Escherichia coli. Journal of Bacteriology 92:328–332
     [Google Scholar]
  9. Dixon M., Webb E.C. 1964 In Enzymes, 2nd edn. pp. 87–90 London: Longmans;
     [Google Scholar]
  10. Enomoto M., Stocker B.A.D. 1974; Trans-duction by phage P1 kc in Salmonella typhimurium. Virology 60:503–514
     [Google Scholar]
  11. Guardiola J., De Felice M., Klopotowski T., Iaccarino M. 1974a; Multiplicity of isoleucine, leucine, and valine transport systems in Escherichia coli K-12. Journal of Bacteriology 117:382–392
     [Google Scholar]
  12. Guardiola J., De Felice M., Klopotowski T., Iaccarino M. 1974b; Mutations affecting the different transport systems for isoleucine, leucine, and valine in Escherichia coli K-12. Journal of Bacteriology 117:393–405
     [Google Scholar]
  13. Hobson A.C. 1974; The regulation of methionine and S-adenosylmethionine biosynthesis and utili-zation in mutants of Salmonella typhimurium with defects in S-adenosylmethionine synthetase. Molecular and General Genetics 131:263–273
     [Google Scholar]
  14. Hobson A.C. 1976; The synthesis of S-Adenosyl methionine by mutants with defects in S-adenosylmethionine synthetase. Molecular and General Genetics 144:87–95
     [Google Scholar]
  15. Hobson A.C., Smith D.A. 1973; S-Adenosyl methionine synthetase in methionine regulatory mutants of Salmonella typhimurium. Molecular and General Genetics 126:7–18
     [Google Scholar]
  16. Holloway C.T., Greene R.C., Su C.-H. 1970; Regulation of S-adenosylmethionine synthetase in Escherichia coli. Journal of Bacteriology 104:734–747
     [Google Scholar]
  17. Iaccarino M., Guardiola J., De Felice M. 1978; On the permeability of biological mem-branes. Journal of Membrane science 3:287–302
     [Google Scholar]
  18. Kadner R.J. 1974; Transport systems for l- methionine in Escherichia coli. Journal of Bacteriology 117:232–241
     [Google Scholar]
  19. Kadner R.J. 1975; Regulation of methionine transport activity in Escherichia coli. Journal of Bacteriology 122:110–119
     [Google Scholar]
  20. Kadner R.J. 1977; Transport and utilization of d-methionine and other methionine sources in Escherichia coli. Journal of Bacteriology 129:207–216
     [Google Scholar]
  21. Kadner R.J., Watson W.J. 1974; Methionine transport in Escherichia coli: physiological and genetic evidence for two uptake systems. Journal of Bacteriology 119:401–409
     [Google Scholar]
  22. Kiritani K. 1974; Mutants of Salmonella typhimurium defective in transport of branched-chain amino acids. Journal of Bacteriology 120:1093–1101
     [Google Scholar]
  23. Kondo E., Mitsuhashi S. 1964; Drug resistance of enteric bacteria. IV. Active transducing bacteriophage P1 CM produced by the com-bination of R factor with bacteriophage P1. Journal of Bacteriology 88:1266–1276
     [Google Scholar]
  24. Lawrence D.A., Smith D.A., Rowbury R.J. 1968; Regulation of methionine synthesis in Salmonella typhimurium: mutants resistant to inhibition by analogues of methionine. Genetics 58:473–492
     [Google Scholar]
  25. Lennox E.S. 1955; Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1:190–206
     [Google Scholar]
  26. Mojica-a T. 1975; Transduction by phage P1 CM clr-100 in Salmonella typhimurium. Molecular and General Genetics 138:113–126
     [Google Scholar]
  27. Oeschger N.S., Hartman P.E. 1970; ICR- induced frameshift mutations in the histidine operon of Salmonella. Journal of Bacteriology 101:490–504
     [Google Scholar]
  28. Ornellas E.P., Stocker B.A.D. 1974; Relation of lipopolysaccharide character to P1 sensitivity in Salmonella typhimurium. Virology 60:491–502
     [Google Scholar]
  29. Osmundsen H. 1975; A computer programme for the determination of the kinetic constants of two enzymes acting simultaneously on the same sub-strate. Biochemical and Biophysical Research Communications 67:324–330
     [Google Scholar]
  30. Payne J.W., Gilvarg C. 1971; Peptide transport. Advances in Enzymology 35:187–244
     [Google Scholar]
  31. Rahmanian M., Claus D.R., Oxender D.L. 1973; Multiplicity of leucine transport systems in Escherichia coli K-12. Journal of Bacteriology 116:1258–1266
     [Google Scholar]
  32. Rosner J.L. 1972; Formation, induction and curing of bacteriophage P1 lysogens. Virology 48:679–689
     [Google Scholar]
  33. Roth J.R. 1970; Genetic techniques in studies of bacterial metabolism. Methods in Enzymology 17A:3–35
     [Google Scholar]
  34. Sanderson K.E., Hartman P.E. 1978; Linkage map of Salmonella typhimurium, edition V. Microbiological Reviews 42:471–519
     [Google Scholar]
  35. Smith H.O., Levine M. 1967; A phage P22 gene controlling integration of prophage. Virology 31:207–216
     [Google Scholar]
  36. Templeton B.A., Savageau M.A. 1974a; Transport of biosynthetic intermediates: homoserine and threonine uptake in Escherichia coli. Journal of Bacteriology 117:1002–1009
     [Google Scholar]
  37. Templeton B.A., Savageau M.A. 1974b; Transport of biosynthetic intermediates: regulation of homoserine and threonine uptake in Escherichia coli. Journal of Bacteriology 120:114–120
     [Google Scholar]
  38. Wild J., Walczak W., Krajewska-Grynkiewicz K., Klopotowski T. 1974; d-Amino acid dehydrogenase: the enzyme of the first step of d-histidine and d-methionine racemization in Salmonella typhimurium. Molecular and General Genetics 128:131–146
     [Google Scholar]
  39. Wood J.M. 1975; Leucine transport in Escherichia coli. Journal of Biological Chemistry 250:4477–4485
     [Google Scholar]
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Methionine Transport in Salmonella typhimurium: Evidence for at Least One Low-affinity Transport System
Microbiology 114, 227 (1979); https://doi.org/10.1099/00221287-114-2-227
/content/journal/micro/10.1099/00221287-114-2-227
/content/journal/micro/10.1099/00221287-114-2-227
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