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SUMMARY: The use of cellulose acetate membrane filters to study fruiting body formation by Myxococcus xanthus is described. This method is used to measure loss and replacement of meso-diamino[14C]pimelic acid during development. Pulse-labelling experiments indicate that fruiting body myxospores resemble those induced by glycerol ( Dawson & Jones, 1979 ) in that cellular morphogenesis is characterized by replacement of existing peptidoglycan by new material.
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