Isolation and Characterization of Methane-utilizing Yeasts Free

Abstract

Summary: Five yeast strains capable of utilizing CH as the sole energy source for growth have been isolated using selective enrichments for methanotrophic micro-organisms. One of the pure cultures was selected for single-cell isolations and antibiotic screening to verify more rigorously the purity of the methanotrophic yeast cultures. Examination of the ultrastructure of this isolate confirmed its eukaryotic nature. The rates of CH oxidation by the isolates were determined by assaying whole cell suspensions for CH-dependent oxygen consumption in an oxygen electrode and by measuring the conversion of CH to CO and incorporation of C into cellular material by whole cells. Characterization of the isolates suggests that four different species of yeast have been obtained that are capable of utilizing CH

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-114-1-187
1979-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/114/1/mic-114-1-187.html?itemId=/content/journal/micro/10.1099/00221287-114-1-187&mimeType=html&fmt=ahah

References

  1. Cooney C. L., Levine D. W. 1972; Microbial utilization of methanol. Advances in Applied Microbiology 15:337–365
    [Google Scholar]
  2. Dawes E. A., McGill D. J., Midgley M. 1971; Analysis of fermentation products. Methods in Microbiology 6A:53–216
    [Google Scholar]
  3. DeVay J. E., Schnarthorst W. C. 1963; Single cell isolation and preservation of bacterial cultures. Nature; London: 199775–777
    [Google Scholar]
  4. Patt T. E., Cole G. C., Bland J., Hanson R. S. 1974; Isolation and characterization of bacteria that grow on methane and organic compounds as sole sources of carbon and energy. Journal of Bacteriology 120:955–964
    [Google Scholar]
  5. Quayle J. R. 1972; The metabolism of one-carbon compounds by microorganisms. Advances in Microbial Physiology 7:119–203
    [Google Scholar]
  6. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  7. Rudd J. W.M., Furutani A., Flett R. J., Hamilton R. D. 1976; Factors controlling methane oxidation in shield lakes: the role of nitrogen fixation and oxygen concentration. Limnology and Oceanography 21:357–364
    [Google Scholar]
  8. Shennan J. L., Levi J. D. 1974; The growth of yeasts on hydrocarbons. Progress in Industrial Microbiology 13:1–57
    [Google Scholar]
  9. Spurr A. R. 1969; A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructural Research 26:31–43
    [Google Scholar]
  10. Volesky B., Zajic J. E. 1971; Batch production of protein from ethane and ethane-methane mixtures. Applied Microbiology 21:614–622
    [Google Scholar]
  11. Walt J. P.van der. 1970; Criteria and methods used in classification. In The Yeasts: A Taxonomic Study pp. 34–113 Lodder J. Edited by Amsterdam: North Holland;
    [Google Scholar]
  12. Whittenbury R. 1971; Hydrocarbons as carbon substrates. In Microbiology 1971 7 pp. 13–24 Hepple P. Edited by London: Institute of Petroleum;
    [Google Scholar]
  13. Whittenbury R., Phillips K. C., Wilkinson J. F. 1970; Enrichment, isolation and some properties of methane-utilizing bacteria. Journal of General Microbiology 61:205–218
    [Google Scholar]
  14. Zajic J. E., Volesky B., Wellman A. 1969; Growth of Graphium sp. on natural gas. Canadian Journal of Microbiology 15:1231–1236
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-114-1-187
Loading
/content/journal/micro/10.1099/00221287-114-1-187
Loading

Data & Media loading...

Most cited Most Cited RSS feed