1887

Abstract

Summary: The tripeptide -methionyl--methionyl--[ C]methionine was taken up into by a saturable system with a pH optimum of 3·5, a temperature optimum of 37 °C and an apparent of 33· 10 M. Metabolic inhibitors such as sodium azide and dinitrophenol completely prevented uptake. Neither methionine nor dimethionine effectively competed with trimethionine uptake. (Leu) Gly-Met-Gly, acetyl-(Met) D-Met--Met--Met and Met-Met-Ile effectively competed with (Met) uptake, whereas (Lys) -Met--Met--Met, -Met--Met--Met, (Met) methyl ester and (Ala) did not. Trimethionine was rapidly hydrolysed by a peptidase after entry into the cell.

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/content/journal/micro/10.1099/00221287-114-1-179
1979-09-01
2024-04-19
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References

  1. Becker J.M., Naider F. 1977; Peptide transport in yeast: uptake of radioactive trimethionine in Saccharomyces cerevisiae. Archives of Biochemistry and Biophysics 178:244–255
    [Google Scholar]
  2. Becker J.M., Naider F., Katchalski E. 1973; Peptide utilization in yeast: studies of methionine and lysine auxotrophs of Saccharomyces cerevisiae. Biochimica et biophysica acta 291:388–397
    [Google Scholar]
  3. Bray G.A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analytical Biochemistry 1:279–285
    [Google Scholar]
  4. Grenson M., Mousset M., Wiame J.M., Bechet J. 1966; Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. Biochimica et biophysica acta 127:325–338
    [Google Scholar]
  5. Lichliter W.D., Naider F., Becker J.M. 1976; Basis for the design of anticandidal agents from studies of peptide utilization in Candida albicans. Antimicrobial Agents and Chemotherapy 10:483–490
    [Google Scholar]
  6. Marder R., Becker J.M., Naider F. 1977; Peptide transport in yeast: utilization of leucine- and lysine-containing peptides by Saccharomyces cerevisiae. Journal of Bacteriology 131:906–916
    [Google Scholar]
  7. Roon R.J., Larimore F., Levy J.S. 1975; Inhibition of amino acid transport by ammonium ion in Saccharomyces cerevisiae. Journal of Bacteriology 124:325–331
    [Google Scholar]
  8. Simoni R.D., Postma P.W. 1975; The energetics of bacterial active transport. Annual Review of Biochemistry 44:523–554
    [Google Scholar]
  9. Ugolev A.M., DeLaey P. 1973; Membrane digestion: a concept of enzymic hydrolysis on cell membranes. Biochimica et biophysica acta 300:105–128
    [Google Scholar]
  10. Vogel H.J., Bonner D.M. 1965; Acetyl ornithinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  11. Wolfinbarger L. Jr Marzluf G.A. 1974; Peptide utilization by amino acid auxotrophs of Neurospora crassa. Journal of Bacteriology 119:371–378
    [Google Scholar]
  12. Wolfinbarger L. Jr Marzluf G.A. 1975; Specificity and regulation of peptide transport in Neurospora crassa. Archives of Biochemistry and Biophysics 171:637–644
    [Google Scholar]
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