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Summary: The tripeptide l-methionyl-l-methionyl-l-[methyl -14C]methionine was taken up into Candida albicans by a saturable system with a pH optimum of 3·5, a temperature optimum of 37 °C and an apparent K m of 33· 10−5 M. Metabolic inhibitors such as sodium azide and dinitrophenol completely prevented uptake. Neither methionine nor dimethionine effectively competed with trimethionine uptake. (Leu)3, Gly-Met-Gly, acetyl-(Met)3, D-Met-l-Met-l-Met and Met-Met-Ile effectively competed with (Met)3 uptake, whereas (Lys)3, l-Met-l-Met-d-Met, d-Met-d-Met-d-Met, (Met)3 methyl ester and (Ala)3 did not. Trimethionine was rapidly hydrolysed by a peptidase after entry into the cell.
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