1887

Abstract

Summary: Some gonococci obtained from human urethral exudate or from subcutaneously implanted chambers in guinea pigs show a resistance to killing by human serum which is lost on subculture after a few generations. The environmental factors which may influence the phenotypic expression of resistance to serum killing were investigated in guinea pig chambers and in chamber fluid

The redox potential in chambers before and after infection was lower than that of heart blood but conditions were not anaerobic; HO increased the redox potential but did not decrease gonococcal serum resistance. The chambers were slightly alkaline before and after infection. When the concentration of glucose (depleted in infected chambers by the abundant polymorphonuclear cells) was restored to excess, the serum resistance of the gonococci was unaffected. Concentrations of free amino acids in chambers changed little during infection. Gonococci adapted to growth in chambers and subsequently rendered serum-sensitive by growing once on agar reverted to serum-resistance after 0·5 to 1 h incubation in chamber fluid at 37 C but not at 25 °C or 4 °C. After 16 to 24 h growth at 37 °C, resistance was again lost. The reversion to serum resistance did not occur in a complex laboratory medium. Examination of the chamber fluid after growth of gonococci showed depletion of lactate, glutamine and proline.

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1979-09-01
2024-03-28
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References

  1. Broome J.D. 1961; Evidence that the l-asparaginase activity of guinea pig serum is responsible for its antilymphoma effects. Nature; London: 1911114–1115
    [Google Scholar]
  2. Chandler F.W., Kraus S., Watts J.C. 1976; Pathological features of experimental gonococcal infection in mice and guinea pigs. Infection and Immunity 13:909–914
    [Google Scholar]
  3. Gibbs D.L., Roberts R.B. 1975; The interaction in vitro between human polymorphonuclear leucocytes and Neisseria gonorrhoeae cultivated in the chick embryo. Journal of Experimental Medicine 141:155–171
    [Google Scholar]
  4. Gutmann I., Wahlefeld A.W. 1974; l-(+)-Lactate. Determination with lactate dehydrogenase and NAD. In Methods of Enzymatic Analysis, 2nd. p. 1464 Bergmeyer H.U. Edited by New York: Academic Press.;
    [Google Scholar]
  5. Hays R.C., Mandell G.L. 1974; p O2, pH and redox potential of experimental abcesses. Proceedings of the Society for Experimental Biology and Medicine 147:29–30
    [Google Scholar]
  6. Hebeler B.H., Morse S.A. 1976; Physiology and metabolism of pathogenic Neisseria: tricarboxylic acid cycle activity in Neisseria gonorrhoeae. Journal of Bacteriology 128:192–201
    [Google Scholar]
  7. Holten E. 1973; Glutamate dehydrogenases in genus Neisseria. Acta pathologica et microbiologica scandinavica 81:49–58
    [Google Scholar]
  8. Holten E. 1976; Radiorespirometric studies in genus Neisseria. 3. The catabolism of pyruvate and acetate. Acta pathologica et microbiologica scandinavica 84:9–16
    [Google Scholar]
  9. Hormaeche R.D., Thornley M.J., Glauert A.M. 1978; Demonstration by light and electron microscopy of capsules on gonococci recently grown in vivo. Journal of General Microbiology 106:81–91
    [Google Scholar]
  10. McCutchan J.A., Levine S., Braude A.I. 1976; Influence of colony type on susceptibility of gonococci to killing by human serum. Journal of Immunology 116:1652–1655
    [Google Scholar]
  11. Morse S.A., Hebeler B.H. 1978; Effect of pH on growth and glucose metabolism of Neisseria gonorrhoeae. In Immunobiology of Neisseria gonorrhoeae pp. 9–17 Brooks G.F., Gotschlich E.C., Holmes K.K., Sawyer W.D., Young F.E. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  12. Muschel L.H., Fong J.S.C. 1977; Serum bactericidal activity and complement. In Biological Amplification Systems in Immunology pp. 137–158 Day N.K., Good R.A. Edited by New York: Plenum.;
    [Google Scholar]
  13. Novotny P., Short J.A., Hughes M., Miler J.J., Syrett C., Turner W.H., Harris J.R.W., MacLennan I.P.B. 1977; Studies on the mechanism of pathogenicity of Neisseria gonorrhoeae. Journal of Medical Microbiology 10:347–365
    [Google Scholar]
  14. Penn C.W., Sen D., Veale D.R., Parsons N.J., Smith H., Witt K. 1976; Morphological, biological and antigenic properties of Neisseria gonorrhoeae adapted to growth in guinea-pig subcutaneous chambers. Journal of General Microbiology 97:35–43
    [Google Scholar]
  15. Penn C.W., Veale D.R., Rittenberg S.C., Smith H. 1977a; The properties of gonococci in vivo and in vitro: selection and phenotypic change in relation to virulence. In Gonorrhoea: Epidemiology and Pathogenesis pp. 117–131 Skinner F.A., Smith H., Walker P.D. Edited by London: Academic Press.;
    [Google Scholar]
  16. Penn C.W., Veale D.R., Smith H. 1977b; Selection from gonococci grown in vitro of a colony type with some virulence properties of organisms adapted in vivo. Journal of General Microbiology 100:147–158
    [Google Scholar]
  17. Rittenberg S.C., Penn C.W., Parsons N.J., Veale D.R., Smith H. 1977; Phenotypic changes in the resistance of Neisseria gonorrhoeae to killing by normal human serum. Journal of General Microbiology 103:69–75
    [Google Scholar]
  18. Schoolnik G.K., Buchanan T.M., Holmes K.K. 1976; Gonococci causing disseminated gonococcal infection are more resistant to the bactericidal action of normal human sera. Journal of Clinical Investigation 58:1163–1173
    [Google Scholar]
  19. Swanson J. 1977; Surface components associated with gonococcal cell interactions. In The Gonococcus pp. 369–402 Roberts R.B. Edited by New York: John Wiley.;
    [Google Scholar]
  20. Taylor P.W. 1978; The effect of the growth environment on the serum sensitivity of some urinary Escherichia coli strains. FEMS Microbiology Letters 3:119–122
    [Google Scholar]
  21. Veale D.R., Smith H., Witt K.A., Marshall R.B. 1975; Differential ability of colonial types of Neisseria gonorrhoeae to produce infection and an inflammatory response in subcutaneous perforated plastic chambers in guinea pigs and rabbits. Journal of Medical Microbiology 8:325–335
    [Google Scholar]
  22. Ward M.E., Watt P.J., Glynn A.A. 1970; Gonococci in urethral exudates possess a virulence factor lost on subculture. Nature; London: 227382–384
    [Google Scholar]
  23. Ward M.E., Lambden P.R., Heckels J.E., Watt P.J. 1978; The surface properties of Neisseria gonorrhoeae: determinants of susceptibility to antibody complement killing. Journal of General Microbiology 108:205–212
    [Google Scholar]
  24. Werner W., Rey H.G., Wielinger H. 1970; Uber die Eigenschaften eines neuen Chromogens für die Blutzucker-Bestimmung nach der GOD/ POD Methode. Zeitschrift für analytische Chemie 252:224–228
    [Google Scholar]
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