1887

Abstract

Summary: Some gonococci obtained from human urethral exudate or from subcutaneously implanted chambers in guinea pigs show a resistance to killing by human serum which is lost on subculture after a few generations. The environmental factors which may influence the phenotypic expression of resistance to serum killing were investigated in guinea pig chambers and in chamber fluid

The redox potential in chambers before and after infection was lower than that of heart blood but conditions were not anaerobic; HO increased the redox potential but did not decrease gonococcal serum resistance. The chambers were slightly alkaline before and after infection. When the concentration of glucose (depleted in infected chambers by the abundant polymorphonuclear cells) was restored to excess, the serum resistance of the gonococci was unaffected. Concentrations of free amino acids in chambers changed little during infection. Gonococci adapted to growth in chambers and subsequently rendered serum-sensitive by growing once on agar reverted to serum-resistance after 0·5 to 1 h incubation in chamber fluid at 37 C but not at 25 °C or 4 °C. After 16 to 24 h growth at 37 °C, resistance was again lost. The reversion to serum resistance did not occur in a complex laboratory medium. Examination of the chamber fluid after growth of gonococci showed depletion of lactate, glutamine and proline.

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/content/journal/micro/10.1099/00221287-114-1-169
1979-09-01
2021-05-15
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