1887

Abstract

SUMMARY: Growth of NC1B 7581 in a simple chemically defined medium was inhibited by -glutamate above 0·01 mg ml; equimolar -glutamate prevented this inhibition. When -glutamate (2 mg ml) was present in the medium (with glycerol as the main carbon source), organisms grew exponentially until all the -isomer had disappeared; growth then stopped for about 24 h during which there was a transient appearance of -glutamine in the medium. Throughout the first stationary phase the concentration of -glutamate in the medium fell continuously and when it was less than 0·01 mg ml there was a second phase of growth.

Exponential phase organisms growing without glutamate contained only 4 m-glutamate in the free amino acid pool. During the first stationary phase with -glutamate added to the medium, the concentration of glutamate (all -isomer) was 47 m in the pool.

Of four other strains of tested, only one was sensitive to -glutamate. From strain 7581 a -glutamate-resistant substrain was easily developed. Among other amino acids added singly to the defined medium, only - (and -) serine was inhibitory to all five strains examined. Inhibition of 7581 by -glutamate was prevented by single addition of several amino acids, each of which could act as a sole source of nitrogen for growth.

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1979-09-01
2021-08-04
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References

  1. Al-ssum R.M., White P.J. 1977; Effects of biotin deficiency on growth, morphology and sporulation in Bacillus megaterium NCIB 7581. Journal of General Microbiology 99:343–351
    [Google Scholar]
  2. Bentley R. 1969 Molecular Asymmetry in Biology 1 pp. 257–271 New York & London: Academic Press;
    [Google Scholar]
  3. Coleman G.S. 1959; The effect ofdl-glutamic acid on the growth of Rhodospirillum rubrum. Biochimica et biophysica acta 31:55–65
    [Google Scholar]
  4. Cosley S.D., McFall E. 1973; Metabolism ofd-serine in Escherichia coli K12: mechanism of growth inhibition. Journal of Bacteriology 114:685–694
    [Google Scholar]
  5. Day A., White P.J. 1977; Enzymic assays for isomers of 2,6-diaminopimelic acid in walls of Bacillus cereus and Bacillus megaterium. Biochemical Journal 161:677–685
    [Google Scholar]
  6. Gale E.F. 1965; l-Amino acids. In Methods of Enzymatic Analysis 2nd edn. pp. 373–377 Bergmeyer H.-U. New York & London: Academic Press;
    [Google Scholar]
  7. Gale E.F., Epps H.M.R. 1944; Studies on bacterial amino-acid decarboxylases. 1. l(+)- Lysine decarboxylase. Biochemical Journal 38:232–242
    [Google Scholar]
  8. Giri K.V., Radhakrishnan A.N., Vaidyana-Than C.S. 1952; Some factors influencing the quantitative determination of amino acids separated by circular paper chromatography. Analytical Chemistry 24:1677–1678
    [Google Scholar]
  9. Glaser L. 1960; Glutamic acid racemase from Lactobacillus arabinosus. Journal of Biological Chemistry 235:2095–2098
    [Google Scholar]
  10. Lark C., Lark K.G. 1961; Studies on the mechanism by whichd-amino acids block cell wall synthesis. Biochimica et biophysica acta 49:308–322
    [Google Scholar]
  11. Rhuland L.E., Work E., Denman R.F., Hoare D.S. 1955; The behaviour of isomers of α,є-diaminopimelic acid on paper chromatograms. Journal of the American Chemical Society 77:4844–4846
    [Google Scholar]
  12. Rydon H.N. 1948; d-Amino-acids in microbio-logical chemistry. Biochemical Society Symposia 1:40–60
    [Google Scholar]
  13. Schales O., Schales S.S. 1955; Amino acid decarboxylases of plants. Methods in Enzymology 2:190–194
    [Google Scholar]
  14. Schleifer K.H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriological Reviews 36:407–477
    [Google Scholar]
  15. Schleifer K.H., Hammes W.P., Kandler O. 1976; Effect of endogenous and exogenous factors on the primary structures of bacterial peptidoglycan. Advances in Microbial Physiology 13:245–292
    [Google Scholar]
  16. Tempest D.W., Meers J.L., Brown C.M. 1970; Influence of environment on the content and composition of microbial free amino acid pools. Journal of General Microbiology 64:171–185
    [Google Scholar]
  17. Tetlow J.A., Wilson A.L. 1964; An absorptiometric method for determining ammonia in boiler feed-water. Analyst 89:453–465
    [Google Scholar]
  18. Tuttle A.L., Gest H. 1960; Induction of morphological aberrations in Rhodospirillum rubrum by d-amino acids. Journal of Bacteriology 79:213–216
    [Google Scholar]
  19. Van der Werf R., Meister A. 1975; The metabolic formation and utilization of 5-oxo-l-proline (l-pyroglutamate,l-pyrrolidone car-boxylate). Advances in Enzymology 43:519–556
    [Google Scholar]
  20. White P.J. 1972; The nutrition of Bacillus megaterium and Bacillus cereus. Journal of General Microbiology 71:505–514
    [Google Scholar]
  21. White P.J. 1979; Effects ofd-glutamate on enzymes of ammonia assimilation in Bacillus megaterium NCIB 7581. Journal of General Microbiology 114:159–168
    [Google Scholar]
  22. White P.J., Lejeune B., Work E. 1969; Assay and properties of diaminopimelate epimerase from Bacillus megaterium. Biochemical Journal 113:589–601
    [Google Scholar]
  23. Wieland O. 1965; Glycerol. In Methods of Enzymatic Analysis, 2nd edn.. pp. 211–214 Bergmeyer H.-U. Edited by New York & London: Academic Press;
    [Google Scholar]
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