1887

Abstract

SUMMARY: Growth of NC1B 7581 in a simple chemically defined medium was inhibited by -glutamate above 0·01 mg ml; equimolar -glutamate prevented this inhibition. When -glutamate (2 mg ml) was present in the medium (with glycerol as the main carbon source), organisms grew exponentially until all the -isomer had disappeared; growth then stopped for about 24 h during which there was a transient appearance of -glutamine in the medium. Throughout the first stationary phase the concentration of -glutamate in the medium fell continuously and when it was less than 0·01 mg ml there was a second phase of growth.

Exponential phase organisms growing without glutamate contained only 4 m-glutamate in the free amino acid pool. During the first stationary phase with -glutamate added to the medium, the concentration of glutamate (all -isomer) was 47 m in the pool.

Of four other strains of tested, only one was sensitive to -glutamate. From strain 7581 a -glutamate-resistant substrain was easily developed. Among other amino acids added singly to the defined medium, only - (and -) serine was inhibitory to all five strains examined. Inhibition of 7581 by -glutamate was prevented by single addition of several amino acids, each of which could act as a sole source of nitrogen for growth.

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1979-09-01
2024-04-25
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