1887

Abstract

Summary: A2 grew on formate as its sole source of carbon and energy. Growth was autotrophic: formate and carbon dioxide were equivalent as carbon sources during growth on formate. Carbon dioxide was fixed by the Calvin cycle in formate- or thiosulphate-grown bacteria which contained comparable high specific activities of ribulosebisphosphate carboxylase. Formate incorporated by bacteria growing heterotrophically or on thio- sulphate showed more restricted metabolism, particularly providing carbon for purines. Bacteria growing on formate or thiosulphate assimilated acetate but showed disproportionately high incorporation into glutamate, proline, arginine, leucine, pyrimidines and lipid. Growth kinetics on formate were studied using extended cultures held at constant formate concentrations at pH 7·8. Yield was a function of substrate concentration and growth rate, which were linearly related in the range 4 to 40 m-formate. Formate was an inhibitory substrate at higher concentrations. A computer analysis of the inhibited growth kinetics indicated and values of 26·5 and 187 m-formate, respectively, and a true of 0·299 h.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-114-1-1
1979-09-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/114/1/mic-114-1-1.html?itemId=/content/journal/micro/10.1099/00221287-114-1-1&mimeType=html&fmt=ahah

References

  1. Anthony C. 1975; The biochemistry of methylotrophic microorganisms. Science Progress 62:167–206
    [Google Scholar]
  2. Badger M.R., Lorimer G.H. 1976; Activation of ribulose-l,5-bisphosphate oxygenase. The role of Mg2+, CO2 and pH. Archives of Biochemistry and Biophysics 175:723–729
    [Google Scholar]
  3. Chandra T.S., Shethna Y.I. 1977; Oxalate, formate, formamide, and methanol metabolism in Thiobacillus novellus. Journal of Bacteriology 131:389–398
    [Google Scholar]
  4. Charles A.M., White B. 1976; Ribulose bis- phosphate carboxylase from Thiobacillus A2. Its purification and properties. Archives of Microbiology 108:195–202
    [Google Scholar]
  5. Cox R.B., Quayle J.R. 1975; The autotrophic growth of Micrococcus denitrificans on methanol. Biochemical Journal 150:569–571
    [Google Scholar]
  6. Dijkhuizen L., Harder W. 1975; Substrate inhibition in Pseudomonas oxalaticus OX1: a kinetic study of growth inhibition by oxalate and formate using extended cultures. Antonie van Leeuwenhoek 41:135–146
    [Google Scholar]
  7. Eccleston M., Kelly D.P. 1973; Assimilation and toxicity of some exogenous C1 compounds, alcohols, sugars and acetate in the methane-oxidizing bacterium Methylococcus capsulatus. Journal of General Microbiology 75:211–221
    [Google Scholar]
  8. Eccleston M., Kelly D.P. 1978; Oxidation kinetics and chemostat growth kinetics of Thiobacillus ferrooxidans on tetrathionate and thiosulphate. Journal of Bacteriology 134:718–727
    [Google Scholar]
  9. Justin P., Kelly D.P. 1978; Growth kinetics of Thiobacillus denitrificans in anaerobic and aerobic chemostat culture. Journal of General Microbiology 107:123–130
    [Google Scholar]
  10. Kelly D.P. 1967; The incorporation of acetate by the chemoautotroph Thiobacillus neapolitanus strain C. Archiv fur Mikrobiologie 58:99–116
    [Google Scholar]
  11. Kelly D.P. 1969; Regulation of chemoauto- trophic metabolism. I. Toxicity of phenylalanine to thiobacilli. Archiv für Mikrobiologie 69:330–342
    [Google Scholar]
  12. Kelly D.P. 1970; Metabolism of organic acids by Thiobacillus neapolitanus. Archiv für Mikrobiologie 73:177–192
    [Google Scholar]
  13. Kelly D.P. 1971; Autotrophy: concepts of litho- trophic bacteria and their organic metabolism. Annual Review of Microbiology 25:177–210
    [Google Scholar]
  14. Kelly D.P. 1973; Metabolic control in litho- trophic bacteria. Journal of General Microbiology 75:vi–vii
    [Google Scholar]
  15. Kelly D.P. 1974; Growth and metabolism of the obligate photolithotroph Chlorobium thiosulfatophilum in the presence of added organic nutrients. Archives of Microbiology 100:163–178
    [Google Scholar]
  16. Kuenen J.G., Veldkamp H. 1972; Thiomicrospira pelophila, gen. n., sp. n., a new obligately chemolithotrophic colourless sulphur bacterium. Antonie van Leeuwenhoek 38:241–256
    [Google Scholar]
  17. Lang E., Lang H. 1972; Spezifische Farbreak- tion zum direkten Nachweis der Ameisensaure. Zeitschrift für analytische Chemie 260:8–10
    [Google Scholar]
  18. London J., Rittenberg S.C. 1966; Effect of organic matter on the growth of Thiobacillus intermedius. Journal of Bacteriology 91:1062–1069
    [Google Scholar]
  19. Peeters T.L., Liu M.S., Aleem M.I.H. 1970; The tricarboxylic acid cycle in Thiobacillus denitrificans and Thiobacillus A2. Journal of General Microbiology 64:29–35
    [Google Scholar]
  20. Pirt S.J. 1965; The maintenance energy of bacteria in growing cultures. Proceedings of the Royal Society B163:224–231
    [Google Scholar]
  21. Quayle J.R. 1972; The metabolism of one carbon compounds by microorganisms. Advances in Microbial Physiology 7:119–203
    [Google Scholar]
  22. Quayle J.R., Keech D.B. 1959; Carbon assimilation by Pseudomonas oxalaticus (OX1). Biochemical Journal 72:623–637
    [Google Scholar]
  23. Roberts R.B., Abelson P.H., Cowie D.B., Bolton E.J., Britten R.J. 1955; Studies on biosynthesis in Escherichia coli. Publications oj the Carnegie Institution of Washington 607:
    [Google Scholar]
  24. Smith A.J., London J., Stanier R.Y. 1967; Biochemical basis of obligate autotrophy in blue-green algae and thiobacilli. Journal of Bacteriology 94:972–983
    [Google Scholar]
  25. Stouthamer A.H. 1973; A theoretical study of the amount of ATP required for synthesis of microbial cell material. Antonie van Leeuwenhoek 39:545–565
    [Google Scholar]
  26. Taylor B.F., Hoare D.S. 1969; New facultative Thiobacillus and a reevaluation of the heterotrophic potential of Thiobacillus novellus. Journal of Bacteriology 100:487–497
    [Google Scholar]
  27. Tuovinen O.H., Kelly D.P. 1973; Studies on the growth of Thiobacillus ferrooxidans. Archiv für Mikrobiologie 88:285–298
    [Google Scholar]
  28. Verseveld H.W., van & Stouthamer A.H. 1978; Growth yields and the efficiency of oxidative phosphorylation during autotrophic growth of Paracoccus denitrificans on methanol and formate. Archives of Microbiology 118:21–26
    [Google Scholar]
  29. Whittenbury R., Kelly D.P. 1977; Autotrophy: a conceptual phoenix. Symposia of the Society for General Microbiology 27:121–149
    [Google Scholar]
  30. Wood A.P., Kelly D.P. 1977; Heterotrophic growth of Thiobacillus A2 on sugars and organic acids. Archives of Microbiology 113:257–264
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-114-1-1
Loading
/content/journal/micro/10.1099/00221287-114-1-1
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error