1887

Abstract

A novel method for obtaining complete lysis of coryneform bacteria has been devised. Bacteria in the late-exponential phase of growth are treated with antibiotics which inhibit biosynthesis of the cell wall. Organisms treated in this way show increased sensitivity to lysis by added lytic enzymes. The preparation of DNA from this group of organisms is thus greatly facilitated.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-113-2-407
1979-08-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/113/2/mic-113-2-407.html?itemId=/content/journal/micro/10.1099/00221287-113-2-407&mimeType=html&fmt=ahah

References

  1. Guerry P., Leblanc D.J., Falkow S. 1973; General methods for the isolation of plasmid deoxyribonucleic acid. Journal of Bacteriology 116:1064–1066
    [Google Scholar]
  2. Huber M.L.B., Godfrey O. 1978; A general method for the lysis of Streptomyces species. Canadian Journal of Microbiology 24:631–632
    [Google Scholar]
  3. Kyowa Hakko Kogyo Ltd 1969; The method of preparation of cell-wall-lysing enzyme (in Japanese). Japan Patent ApplicationS44–6624
    [Google Scholar]
  4. Schneider K., Schlegel H.G. 1977; Localization and stability of hydrogenases from aerobic hydrogen bacteria. Archives of Microbiology 112:229–238
    [Google Scholar]
  5. Schrempf H., Bujard H., Hopwood D.A., Goebel W. 1975; Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2). Journal of Bacteriology 121:416–421
    [Google Scholar]
  6. Yamada K., Komagata K. 1970; Taxonomic studies on coryneform bacteria. III. DNA base composition of coryneform bacteria. Journal of General and Applied Microbiology 16:215–224
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-113-2-407
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error