1887

Abstract

Summary: When sucrose or acetate was added to washed suspensions of a number of aerobic bacteria, exogenous ferricytochrome was reduced, luminol chemiluminescence was enhanced and the rate of adrenaline auto-oxidation was increased. Superoxide dismutase from bovine erythrocytes inhibited all of there reactions, suggesting that superoxide radicals (O ) are formed during aerobic bacterial metabolism. Superoxide radicals were probably generated during respiratory chain activity because O production by intact bacteria was stimulated by antimycin A and the highest O -producing activity was observed with membrane fractions with a high concentration of cytochromes. Exogenous superoxide dismutase partially inhibited NADH-dependent oxgyen consumption by membrane particles of 22LD.

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1979-08-01
2024-04-20
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References

  1. Babior B.M., Kipnes R.S., Curnutte J.T. 1973; The production by leucocytes of superoxide, a potential bactericidal agent. Journal of Clinical Investigation 52:741–744
    [Google Scholar]
  2. Boveris A., Cadenas E. 1975; Mitochondrial production of superoxide anions and its relationship to the antimycin insensitive respiration. FEBS Letters 54:311–314
    [Google Scholar]
  3. Boveris A., Cadenas E., Stoppani A. 1976; Role of ubiquinone in the mitochondrial generation of hydrogen peroxide. Biochemical Journal 156:435–444
    [Google Scholar]
  4. Buchanan A.G. 1977; The response of Azotobacter chroococcum to oxygen: superoxide-mediated effects. Canadian Journal of Microbiology 23:1548–1553
    [Google Scholar]
  5. Cadenas E., Boveris A., Ragan C.I., Stoppani A.O.M. 1977; Production of superoxide radicals and hydrogen peroxide by NADH- ubiquinone reductase and ubiquinol-cytochrome c reductase from beef-heart mitochondria. Archives of Biochemistry and Biophysics 180:248–257
    [Google Scholar]
  6. Chance B., Saronio C., Leigh J.S.Jr 1975; Functional intermediates in reaction of cytochrome oxidase with oxygen. Proceedings of the National Academy of Sciences of the United States of America 72:1635–1640
    [Google Scholar]
  7. Devlin R.G., Lin C.S., Perper R.J., Dougherty H. 1977; Is superoxide anion production involved in lymphocyte killing of target cells?. Federation Proceedings 36:1065
    [Google Scholar]
  8. Fridovich I. 1972; Superoxide radical and superoxide dismutase. Accounts of Chemical Research 5:321–326
    [Google Scholar]
  9. Fridovich I. 1974; Superoxide dismutase. In Molecular Mechanisms of Oxygen Activation pp. 453–477 Hayashi O. Edited by New York and London: Academic Press.;
    [Google Scholar]
  10. Fridovich I. 1975; Oxygen: boon and bane. American Scientist 63:54–59
    [Google Scholar]
  11. Goldstein I.M., Cerqueira M., Lind S., Kaplan H.B. 1977; Evidence that the superoxide generating system of human leucocytes is associated with the cell surface. Journal of Clinical Investigation 59:248–254
    [Google Scholar]
  12. Gregory E.M., Fridovich I. 1973; Oxygen toxicity and the superoxide dismutase. Journal of Bacteriology 114:1193–1197
    [Google Scholar]
  13. Hodgson E.K., Fridovich I. 1973; The role of O2 in the chemiluminescence of luminol. Photochemistry and Photobiology 18:451–455
    [Google Scholar]
  14. Jones D., Watkins J., Meyer D.J. 1970; Cytochrome composition and effect of catalase on growth of Agromyces ramnosus. Nature; London: 2261249–1250
    [Google Scholar]
  15. Kristapsons M.Ž., Viesturs U.E. 1973; FS-5A stand for aerobic microorganism cultivation. In Upravliayemiy Mikrobniy Syntez pp. 71–76 Riga: Zinātne.; in Russian
    [Google Scholar]
  16. Loschen G., Azzi A., Richter CH., Flohe L. 1974; Superoxide radicals as precursors of mitochondrial hydrogen peroxide. FEBS Letters 42:68–72
    [Google Scholar]
  17. Low I.E., Eaton M.D., Proctor P. 1968; Relation of catalase to substrate utilization by Mycoplasma pneumoniae. Journal of Bacteriology 95:1425–1430
    [Google Scholar]
  18. Mccord J.M., Fridovich I. 1969; Superoxide dismutase, an enzymic function of erythrocuprein (hemocuprein). Journal of Biological Chemistry 244:6049–6055
    [Google Scholar]
  19. Merzliak M.N., Sobolev A.S. 1975; The role of superoxide anion radicals and singlet oxygen in membrane pathology. In Itogy nauky itekhniky, Biophysika 6 pp. 118–165 Moscow: VINITI; in Russian
    [Google Scholar]
  20. Michelson A.M. 1973; Studies in bioluminescence.Chemical models of enzymic oxidations. Biochimie 55:465–479
    [Google Scholar]
  21. Misra H.P., Fridovich I. 1972; The role of superoxide anions in the autooxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological Chemistry 247:3170–3175
    [Google Scholar]
  22. Shvinka J.E., Toma M.K. 1977; Branched electron transport chain in Brevibacterium sp. 22 LD. In Upravleniye Mikrobnim Syntezom pp. 5–11 Riga: Zinaātne; in Russian
    [Google Scholar]
  23. Shvinka J.E., Zvirgzdina L.K., Toma M.K. 1978; Characteristics of membrane vesicles isolated from Brevibacterium sp. 22 LD. In Produtsenty Aminokislot i Fermentov pp. 30–37 Riga: Zinātne; in Russian
    [Google Scholar]
  24. Sugijama Y., Kitano K., Kanzaki T. 1973; Purification of cytochrome a of a l-glutamate- producing microorganism Brevibacterium thiogenitalis. Agricultural and Biological Chemistry 37:1607–1612
    [Google Scholar]
  25. Van Hemmen J.J., Meuling W.J.A. 1977; Inactivation of Escherichia coli by superoxide radicals and their dismutation products. Archives of Biochemistry and Biophysics 182:743–748
    [Google Scholar]
  26. Weser U., Bunnenberg E., Cammack R., Djerassi C., Flohe L., Thomas G., Voelter W. 1971; A study on purified bovine erythrocuprein. Biochimica et biophysicaacta 243:203–213
    [Google Scholar]
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