Microbial Determinations by Flow Cytometry Free

Abstract

Summary: Recent improvements in the optics and electronics of flow cytometry systems, as well as in staining techniques, permit the assay of such minute cellular constituents as the DNA and protein contents of micro-organisms. To assess the usefulness of this technique, DNA and protein content distributions were determined in 8k, . indicated that the method can be used to determine ploidy. The rapidity of flow cytometry measurements allows accurate determinations in large populations.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-113-2-369
1979-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/113/2/mic-113-2-369.html?itemId=/content/journal/micro/10.1099/00221287-113-2-369&mimeType=html&fmt=ahah

References

  1. Bailey J. E., Fazel-Madjlessi J., Mcquitty D. N., Lee L. Y., Allred J. C., Oro J. A. 1977; Characterization of bacterial growth by means of flow microfluorometry. Science 198:1175–1176
    [Google Scholar]
  2. Dittrich W., Göhde W. 1969; Impulsfluorometrie bei Einzelzellen in Suspension. Zeitschrift für Naturforschung 24b:360–361
    [Google Scholar]
  3. Duffus J. H. 1971; The cell cycle in yeast. Journal of the Institute of Brewing 77:500–508
    [Google Scholar]
  4. Emeis C.-C. 1962; Aneuploide Hefen. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Medizinische Originale) 184:247–250
    [Google Scholar]
  5. Esser K., Kuenen R. 1965 Genetics of Fungi. Berlin: Springer Verlag.;
    [Google Scholar]
  6. Falchuk K. H., Krishan A., Vallee B. L. 1975; DNA distribution in the cell cycle of Euglena gracilis. Cytofluorometry of zinc deficient cells. Biochemistry 14:3439–3444
    [Google Scholar]
  7. Göhde W. 1972; Automation in der quantitativen Zytologie mit dem Impulscytophotometer. GBK Mitteilungsdienst 6:255–276
    [Google Scholar]
  8. Halvorson H. O., Carter B. L.A., Tauro P. 1971; Synthesis of enzymes during the cell cycle. Advances in Microbial Physiology 6:47–106
    [Google Scholar]
  9. Hartwell L. H. 1974; Saccharomyces cerevisiae cell cycle. Bacteriological Reviews 38:164–198
    [Google Scholar]
  10. Howard A., Pelc S. R. 1953; Synthesis Of deoxyribonucleic acid in normal and irradiated cells and its relation to chromosome breakage. Heredity 6: Suppl. 261–273
    [Google Scholar]
  11. Hutter K.-J. 1974 Untersuchungen über die DNS-, RNS- und Proteinsynthese von Hefezellen der Gattung Saccharomyces mit Hilfe neuer fluorometrischer Verfahren. Dissertation, T. U., Berlin 19/13
    [Google Scholar]
  12. Hutter K.-J. 1975; Ploidiegradbestimmung von Saccharomyces-Hefen.. Chemie, Mikrobiologie, Technologie der Lebensmittel 4:105–109
    [Google Scholar]
  13. Hutter K.-J., Eipel H. E. 1978a; DNA distribution of yeast by flow cytometry. FEMS Microbiology Letters 3:35–38
    [Google Scholar]
  14. Hutter K.-J., Eipel H. E. 1978b; Protein content distribution in population of baker’s yeast. European Journal of Applied Microbiology and Biotechnology 5:203–206
    [Google Scholar]
  15. Hutter K.-J., Görtz H. T., Oldiges H., Eipel H. E. 1978a; Durchflusszytophotometrische Bestimmung des DNS-Gehaltes von Nectria coccinea Pers. ex Fr. bei Fungizideinwirkung. Chemosphere 7:51–58
    [Google Scholar]
  16. Hutter K.-J., Görtz H. T., Eipel H. E. 1978b; Different stages of DNA synthesis during the growth of Saccharomyces cerevisiae.. FEMS Microbiology Letters 3:291–294
    [Google Scholar]
  17. Kamentsky L. A. 1970 Rapid Cell Spectrophotometer for Cell Identification and Sorting. Evans D. M.D. Edited by London: E & S Livingstone.;
    [Google Scholar]
  18. Kamentsky L. A., Melamed M. R., Derman H. 1965; Spectrophotometer: new instrument for ultrarapid cell analysis. Science 150:630–631
    [Google Scholar]
  19. Kessler E., Czygan F. C. 1970; Physiologische und biochemische Beiträge zur Taxonomie der Gattung Chlorella.. Archiv für Mikrobiologie 70:211–216
    [Google Scholar]
  20. Kornberg A. 1974 ed DNA Synthesis. San Francisco: W. H. Freeman.;
    [Google Scholar]
  21. Laskowski W. 1962; Über den Aufbau weitestgehend isogener, homozygoter penta- und hexa- ploider Stämme sowie den Einfluss bestimmter mutierter Allele auf die Strahlenresistenz. Zeitschrift für Naturforschung 17b:93–108
    [Google Scholar]
  22. Laskowski W., Lochmann E. R., Wacker A., Stein W. 1960; Biochemische Eigcnschaften in Abhängigkeit vom Ploidiegrad und Kombination der Paarungstypallele. Zeitschrift für Naturforschung 15b:730–734
    [Google Scholar]
  23. Mitchison J. M. 1971 Biology of the Cell Cycle. Cambridge: Cambridge University Press.;
    [Google Scholar]
  24. Paau A. S., Lee D., Cowles J. R. 1977a; Comparison of nucleic acid content in populations of free-living and symbiotic Rhizobium meliloti by flow microfluorometry. Journal of Bacteriology 129:1156–1158
    [Google Scholar]
  25. Paau A. S., Cowles J. R., Oro J. 1977b; Flow-microfluorometric analysis of Escherichia coli, Rhizobium meliloti, and Rhizobium japonicum at different stages of the growth cycle. Canadian Journal of Microbiology 23:1165–1169
    [Google Scholar]
  26. Skogen Hagenson M. J. 1976 A high efficiency flow microfluorometer: application to bacterial fluorescence. MS thesis Iowa State University,Ames, U. S.A.:
    [Google Scholar]
  27. Slater M. L., Sharrow S. O., Gart J. J. 1977; Cell cycle of Saccharomyces cerevisiae in populations growing at different stages. Proceedings of the National Academy of Sciences of the United States of America 74:3850–3854
    [Google Scholar]
  28. Smith J. E., Berry D. R. 1974 (editors) An Introduction to the Biochemistry of Fungal Development. London &New York: Academic Press.;
    [Google Scholar]
  29. Streble E., Krauter D. 1974 editors Das Leben im Wassertropfen. Stuttgart: Franckh’sche Verlagsbuchhandlung.;
    [Google Scholar]
  30. Strugger S. 1949 Fluoreszenzmikroskopie und Mikrobiologie. Hanover: M. & S. Schaper.;
    [Google Scholar]
  31. Williamson D. H. 1965; The timing of deoxyribonucleic acid synthesis in the cell cycle of Saccharomyces cerevisiae.. Journal of Cell Biology 25:517–528
    [Google Scholar]
  32. Williamson D. H., Scopes A. W. 1961; Protein synthesis and nitrogen uptake in synchronously dividing culture of Saccharomyces cerevisiae.. Journal of the Institute of Brewing 67:39–42
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-113-2-369
Loading
/content/journal/micro/10.1099/00221287-113-2-369
Loading

Data & Media loading...

Most cited Most Cited RSS feed