1887

Abstract

Summary: The size of the amino acid pool in , a rumen bacterium, was limited by the availability of ammonia in the growth medium. Alanine was the major pool constituent irrespective of the growth-limiting nutrient. In steady-state ammonia-limited chemostat cultures, glutamine synthetase (GS) activity was tenfold higher and glutamate dehydrogenase (GDH) activity up to fivefold lower than in maltose-limited cultures. No glutamate synthase (GOGAT) activity was demonstrated. When excess ammonia was pulsed into an ammonia-limited chemostat culture, the glutamine pool expanded rapidly and GS was inactivated. This was consistent with the observation that, in a number of bacteria, GS functions to assimilate ammonia when the prevailing concentration is low. However, GDH was always very active in extracts of and its Michaelis constant for ammonia was relatively low (1 to 2 m). This suggested that GDH could continue to function bio-synthetically when GS was derepressed.

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1979-08-01
2024-04-24
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References

  1. Allison M. J. 1970; Nitrogen metabolism oiruminal micro-organisms. In Physiology of Digestion and Metabolism in the Ruminant pp. 456–473 Phillipson A. T. Edited by Newcastle-upon-Tyne: Oriel Press;
    [Google Scholar]
  2. Atkin G. E., Ferdinand W. 1970; Accelerated amino acid analysis.Studies on the use of lithium citrate buffers and the effect of n-propanol in the analysis of physiological fluids and protein hydrolysates. Analytical Biochemistry 38:313–329
    [Google Scholar]
  3. Bender R. A., Janssen K. A., Resnik A. D., Blumenberg M., Foor F., Magasanik B. 1977; Biochemical parameters of glutamine synthetase from Klebsiella aerogenes. Journal of Bacteriology 129:1001–1009
    [Google Scholar]
  4. Blackburn T. H. 1965 Protease Production by Bacteroidesamylophilus, a Rumen Bacterium Ph.D. thesis University of Aberdeen:
    [Google Scholar]
  5. Blackburn T. H. 1968; Protease production by Bacteroides amylophilus strain H-18. Journal of General Microbiology 53:27–36
    [Google Scholar]
  6. Brown C. M., Stanley S. O. 1972; Environment mediated changes in the cellular content of the ‘pool’ constituents and their associated changes in cell physiology. In Environmental Control of Cell Synthesis and Function pp. 363–389 Dean A. C. R., Pirt S. J., Tempest D. W. Edited by London: Academic Press;
    [Google Scholar]
  7. Brown C. M., Macdonald-Brown D. S., Meers J. L. 1974; Physiological aspects of microbial inorganic nitrogen metabolism. Advances in Microbial Physiology 11:1–52
    [Google Scholar]
  8. Buttery P. J., Annison E. F. 1973; Considerations of the efficiency of amino acid and protein metabolism in animals. In The Biological Efficiency of Protein Production part III pp. 145–171 Jones J. G. W. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  9. Caldwell D. R., Keeney M., Van Soest P. J. 1969; Effects of CO2 on growth and maltose fermentation by Bacteroides amylophilus. Journal of Bacteriology 98:668–676
    [Google Scholar]
  10. Chalupa W., Clark J., Opliger P., Lavker R. 1970; Ammonia metabolism in rumen bacteria and mucosa from sheep fed soy protein or urea. Journal of Nutrition 100:161–169
    [Google Scholar]
  11. Cole J. A., Coleman K. J., Compton B. E., Kavanagh B. M., Keevil C.W. 1974; Nitrite and ammonia assimilation by anaerobic continuous cultures of Escherichia coli. Journal of General Microbiology 85:11–22
    [Google Scholar]
  12. Conway E. J. 1962 Microdiffusion Analysis and Volumetric Error, 5th edn.. London: Crosby Lockwood & Son;
    [Google Scholar]
  13. Dainty R.H. 1972; Glutamate biosynthesis in Clostridium pasteurianum and its significance in nitrogen metabolism. Biochemical Journal 126:1055–1056
    [Google Scholar]
  14. Dawson P. S. S. 1965; The intracellular amino acid pool of Candida utilis during growth in batch and continuous flow culture. Biochimica et biophysica acta 111:51–56
    [Google Scholar]
  15. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  16. Griffith C. J., Carlsson J. 1974; Mechanism of ammonia assimilation in streptococci. Journal of General Microbiology 82:253–260
    [Google Scholar]
  17. Henderson C., Hobson P. N., Summers R. 1969; The production of amylase, protease and lipolytic enzymes by two species of anaerobic rumen bacteria. In Continuous Cultivation of Micro-organisms pp. 189–204 Malek I. Prague: Academia;
    [Google Scholar]
  18. Hobson P. N. 1965; Continuous culture of rumen bacteria: apparatus. Journal of General Microbiology 38:161–166
    [Google Scholar]
  19. Hobson P. N., Summers R. 1967; The continuous culture of anaerobic bacteria. Journal of General Microbiology 47:53–65
    [Google Scholar]
  20. Hughes D. E. 1951; A press for disrupting bacteria and other micro-organisms. British Journal of Experimental Pathology 32:97–109
    [Google Scholar]
  21. Hullah W. A., Blackburn T. H. 1971; Uptake and incorporation of amino acids and peptides by Bacteroides amylophilus. Applied Microbiology 21:187–191
    [Google Scholar]
  22. Hungate R. E. 1966 The Rumen and Its Microbes London: Academic Press;
    [Google Scholar]
  23. Jones K., Thomas J. G. 1974; Nitrogen fixation by the rumen contents of sheep. Journal of General Microbiology 85:97–101
    [Google Scholar]
  24. Joyner A. E.Jr Baldwin R. L. 1966; Enzymatic studies of pure cultures of rumen microorganisms. Journal of Bacteriology 92:1321–1330
    [Google Scholar]
  25. Latham M. J., Sharpe E. M. 1971; The isolation of anaerobic organisms from the bovine rumen. In Isolation of Anaerobes pp. 132–147 Shapton D. A., Board R. G. Edited by London: Academic Press;
    [Google Scholar]
  26. Lewis D., Swan H. 1971; The role of intestinal flora in animal nutrition. Symposia of the Society for General Microbiology 21:149–175
    [Google Scholar]
  27. Magasanik B., Prival M. J., Brenchley J. E., Tyler B. M., Deleo A. M., Streicher S. L., Bender R. A., Paris C. G. 1974; Glutamine synthetase as a regulator of enzyme synthesis. Current Topics in Cellular Regulation 8:119–138
    [Google Scholar]
  28. Meers J. L., Tempest D. W., Brown C. M. 1970; ‘Glutamine(amide):2-oxoglutarate amino transferaseoxido-reductase (NADP)’, an enzyme involved in the synthesis of glutamate by some bacteria. Journal of General Microbiology 64:187–194
    [Google Scholar]
  29. Nyberg K., Clark P. H. 1978; Glutamine synthetase activities of cultures of Pseudomonas aeruginosa grown in minimal media with histidine, nitrate or ammonium sulphate as the nitrogen source. Journal of General Microbiology 107:193–197
    [Google Scholar]
  30. Pulman D., Johnson B. 1978; The enzymes of ammonia assimilation and their control in members of the genus Erwinia. Journal of General Microbiology 106:137–143
    [Google Scholar]
  31. Reeves H. C., Rabin R., Wegener W. S., Ajl S. J. 1971; Assays of enzymes of the tricarboxylic acid and glyoxylate cycles. Methods in Microbiology 6A:425–462
    [Google Scholar]
  32. Rowe W. B., Ronzio R. A., Wellner V. P., Meister A. 1970; Glutamine synthetase (sheep brain). Methods in Enzymology 17A:900–910
    [Google Scholar]
  33. Shapiro B. M., Stadtman E. R. 1970a; The regulation of glutamine synthesis in microorganisms. Annual Review of Microbiology 24:501–524
    [Google Scholar]
  34. Shapiro B. M., Stadtman E. R. 1970b; Glutamine synthetase (E. coli). Methods in Enzymology 17A:910–922
    [Google Scholar]
  35. Tempest D. W., Meers J. L., Brown C. M. 1970; Influence of environment on content and composition of microbial free amino acid pools. Journal of General Microbiology 64:171–185
    [Google Scholar]
  36. Tyler B. 1978; Regulation of the assimilation of nitrogen compounds. Annual Review of Biochemistry 47:1127–1162
    [Google Scholar]
  37. Umbarger H. E. 1969; The regulation of amino acid metabolism. Annual Review of Biochemistry 38:323–370
    [Google Scholar]
  38. Wohlhueter R. M., Schutt H., Holzer H. 1973; Regulation of glutamine synthetase in vivo in Escherichia coli. In The Enzymes of Glutamine Metabolism pp. 45–64 Prusiner S., Stadtman E. R. Edited by New York: Academic Press;
    [Google Scholar]
  39. Woolfolk C. A., Shapiro B., Stadtman E. R. 1966; Regulation of glutamine synthetase. I. Purification and properties of glutamine synthetase from Escherichia coli. Archives of Biochemistry and Biophysics 116:177–192
    [Google Scholar]
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