1887

Abstract

The cell volume at bud initiation for both haploid and diploid cells of is dependent on growth rate within the mass doubling time range 2·1 to 3·7 h. At slower growth rates, the volume at bud initiation is independent of growth rate. At all growth rates, the volume at bud initiation for diploids is 1·7-fold larger than that for haploids. When unbudded cells from synchronous cultures growing in poor media are shifted to rich medium, all except those very close to the size characteristic of bud production on poor medium go on to produce a bud at the larger size characteristic of the richer medium. Cells do not become committed to producing a bud at the size characteristic of bud initiation on ethanol medium (a poor medium) until they are within about 3 m of that size. When unbudded cells are shifted from rich medium to poor medium, cells that are smaller than the size of bud initiation on the poor medium produce a bud at the size characteristic of the poor medium. If they are larger than the commitment size for the poor medium at the time of the shift, they produce a bud without appreciable growth. These results are examined in relation to the unstable inhibitor model for the control of cell division.

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/content/journal/micro/10.1099/00221287-113-2-287
1979-08-01
2021-07-31
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References

  1. Adams J. 1977; I he interrelationship ot cell growth and division in haploid and diploid cells of Saccharomyces cerevisiae.. Experimental Cell Research 106:267–275
    [Google Scholar]
  2. Carter B.L.A. 1978; The yeast nucleus. Advances in Microbial Physiology 17:244–297
    [Google Scholar]
  3. Carter B.L.A., Jagadish M.N. 1978; The relationship between cell size and cell division in the yeast Saccharomyces cerevisiae.. Experimental Cell Research 112:15–24
    [Google Scholar]
  4. Carter B.L.A., Sebastian J., Halvorson H.O. 1971; The regulation of the synthesis of arginine catabolizing enzymes during the cell cycle in Saccharomyces cerevisiae.. Advances in Enzyme Regulation 9:253–263
    [Google Scholar]
  5. Fantes P.A., Grant W.D., Pritchard R.H., Sudbery P.E., Wheals A.E. 1975; The regulation of cell size and the control of mitosis. Journal of Theoretical Biology 50:213–244
    [Google Scholar]
  6. Frazier E.A.J. 1973; DNA synthesis following gross alterations of the nucleocytoplasmic ratio in the ciliate Stentor coerulus.. Developmental Biology 34:77–92
    [Google Scholar]
  7. Halvorson H.O., Carter B.L.A., Tauro P. 1971; Use of synchronous cultures of yeast to study gene position. Methods in Enzymology 21:462–470
    [Google Scholar]
  8. Hartmann M. 1928; Über experimentelle Unster blichkeit von Protozoen-Individuen. Ersatz der Fortpflanzung von Amoeba proteus durch fortgesetzte Regenera tionen. Zoologische Juhrbücher 45:973–987
    [Google Scholar]
  9. Hartwell L.H., Unger M.W. 1977; Unequal cell division in Saccharomyces cerevisiae and its implications for the control of cell division. Journal of Cell Biology 75:422–435
    [Google Scholar]
  10. Hartwell L.H., Mortimer R.K.., Culotti J., Culotti M. 1973; Genetic control of the cell division cycle in yeast. V. Genetic analysis of cdc mutants. Genetics 74:267–286
    [Google Scholar]
  11. Jagadish M.N., Carter B.L.A. 1977; Genetic control of cell division in yeast cultured at different growth rates. Nature; London: 269145–147
    [Google Scholar]
  12. Jagadish M.N., Lorincz A.L., Carter B.L.A. 1977; Cell size and cell division in yeast cultured at different growth rates. FEMS Microbiology Letters 2:235–237
    [Google Scholar]
  13. Johnston G.C., Pringle J.R., Hartwell L.H. 1977; Coordination of growth with cell division in the yeast Saccharomyces cerevisiae.. Experimental Cell Research 105:79–98
    [Google Scholar]
  14. Meyenburg H.K.von. 1968; The budding cycle of Saccharomyces cerevisiae.. Pathologia microbiologica 31:117–127
    [Google Scholar]
  15. Mitchison J.M. 1977; The timing of cell cycle events. In Mitosis, Facts and Questions pp. 1–13 Little M., Laweletz N., Petzelt C., Ponstingle H., Schroeter D., Zimmerman H.-P. Edited by Berlin: Springer-Verlag.;
    [Google Scholar]
  16. Nurse P. 1975; Genetic control of cell size at cell division in yeast. Nature; London: 256547–551
    [Google Scholar]
  17. Prescott D.M. 1956; Relation between cell growth and division. III. Changes in nuclear volume and growth rate, and prevention of cell division in Amoeba proteus resulting from cytoplasmic amputations. Experimental Cell Research 11:94–98
    [Google Scholar]
  18. Pritchard R.H., Barth P.T., Collins J. 1969; Control of DNA synthesis in bacteria. Symposia of the Society for General Microbiology 19:263–297
    [Google Scholar]
  19. Sebastian J., Carter B.L.A., Halvorson H.O. 1971; Use of yeast populations fractionated by zonal centrifugation to study the cell cycle. Journal of Bacteriology 108:1045–1050
    [Google Scholar]
  20. Sudbery P.E., Grant W.D. 1975; The control of mitosis in Physarum polycephalum. The effect of lowering the DNA: mass ratio by U.V. irradiation. Experimental Cell Research 95:405–415
    [Google Scholar]
  21. Ycas M., Sugita M., Bensam A. 1965; A model of cell size regulation. Journal of Theoretical Biology 9:444–470
    [Google Scholar]
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