1887

Abstract

Mutants of with altered resistance to low molecular weight organic solvents were isolated. Solvent-resistant mutants showed a decrease in the ratio of phosphatidyl-ethanolamine to the anionic phospholipids (phosphatidylglycerol and cardiolipin) relative to the wild-type, whereas solvent-sensitive strains showed an increase. Reversion studies on representative mutants demonstrated that the phenotypic response to solvents and the changes in phospholipid composition were genetically associated. The fatty acid and lipo-polysaccharide compositions of the various mutants showed no significant differences from the parental strain. The lesions in two of the solvent-sensitive mutants (DC7 and DC9) and one of the resistant mutants (DC11) were mapped by cotransduction with phage P1 and shown to lie very close to the locus at 56 min on the map.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-113-2-267
1979-08-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/113/2/mic-113-2-267.html?itemId=/content/journal/micro/10.1099/00221287-113-2-267&mimeType=html&fmt=ahah

References

  1. Adelberg E.A., Mandel M., Chen G.C.C. 1965; Optimal conditions for mutagenesis by N-methyl-N′-nitrosoguanidine in Escherichia coli K12. Biochemical and Biophysical Research Communications 18:788–790
    [Google Scholar]
  2. Aminoff D. 1961; Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochemical Journal 81:384–393
    [Google Scholar]
  3. Bachmann B.J. 1972; Pedigrees of some mutant strains of E. coli K12. Bacteriological Reviews 36:525–557
    [Google Scholar]
  4. Bachmann B.J., Low K.B., Taylor A.L. 1976; Recalibrated linkage map of Escherichia coli K-12. Bacteriological Reviews 40:116–167
    [Google Scholar]
  5. Beard J.P., Conolly J.C. 1975; Detection of a protein similar to the sex pilus subunit in the outer membrane of E. coli cells carrying a derepressed F-like R-factor. Journal of Bacteriology 122:59–65
    [Google Scholar]
  6. Bhatti T., Chambers R., Clamp J.R. 1970; The gas chromatographic properties of biologically important N-acetylglucosamine derivatives, monosaccharides, disaccharides, trisaccharides, tetrasaccharides and pentasaccharides. Biochimica et biophysica acta 222:339–347
    [Google Scholar]
  7. Bligh E.G., Dyer W.T. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 39:911–917
    [Google Scholar]
  8. Brindley D.N., Allan D., Michell R.M. 1975; The redirection of glyceride and phospholipid synthesis by drugs including chlorpromazine, fenfluramine, imipramine, mepyramine and local anaesthetics. Journal of Pharmacy and Pharmacology 27:462–464
    [Google Scholar]
  9. Chance B., Lee C.-P., Blasie J.K. 1971 Probes of Structure and Function of Macro-molecules and Membranes I London and New York: Academic Press.;
    [Google Scholar]
  10. Chen P.S., Toribara T.Y., Warner H. 1956; Microdetermination of phosphorus. Analytical Chemistry 28:1956–1958
    [Google Scholar]
  11. Cronan J.E.Jr Vagelos P.R. 1972; Metabolism and function of the membrane phospho-lipids of E. coli.. Biochimica et biophysica acta 265:25–60
    [Google Scholar]
  12. Cronan J.E.Jr Silbert D.F., Wulff D.L. 1972; Mapping of the fabA locus for unsaturated fatty acid biosynthesis in E. coli.. Journal of Bacteriology 112:206–211
    [Google Scholar]
  13. Dagley S., Dawes E.A., Morrison G.A. 1950; Inhibition of growth of Aerobacter aerogenes: the mode of action of phenols, alcohols, acetone and ethyl acetate. Journal of Bacteriology 60:369–379
    [Google Scholar]
  14. Fried V.A., Novick A. 1973; Organic solvents as probes for the structure and function of the bacterial membrane: effects of ethanol on the wild type and ethanol resistant mutant of E. coli K12. Journal of Bacteriology 114:239–248
    [Google Scholar]
  15. Galanos C., Lüderitz O., Westphal O. 1969; A new method for the extraction of rough lipo-polysaccharide. European Journal of Biochemistry 9:245–249
    [Google Scholar]
  16. Harold F.M. 1970; Antimicrobial agents and membrane function. Advances in Microbial Physiology 4:45–104
    [Google Scholar]
  17. Hill M.W. 1974; The effect of anaesthetic like molecules on the phase transition in smectic mesophases of dipalmitoyl lecithin. I. The normal alcohols up to C = 9 and three inhalation anaesthetics. Biochimica et biophysica acta 356:117–124
    [Google Scholar]
  18. Hugo W.B. 1967; The mode of action of antibacterial agents. Journal of Applied Bacteriology 30:17–50
    [Google Scholar]
  19. Lenaz G., Bertoli E., Curabola G., Mazzanti L., Bigi A. 1976; Lipid protein interactions in mitochondria: spin and fluorescence probe studies on the effects of n-alkanols on phospholipid vesicles and mitochondrial membranes. Archives of Biochemistry and Biophysics 172:278–288
    [Google Scholar]
  20. Low B. 1973; Rapid mapping of conditional and auxotrophic mutations in E. coli K-12. Journal of Bacteriology 113:798–812
    [Google Scholar]
  21. Nikaido M., Nikaido K., Rapin A.M.C. 1965; Biosynthesis of thymidine diphosphate-l-rhamnose in E. coli K12. Biochimica et biophysica acta 111:548–551
    [Google Scholar]
  22. Norris J.R., Ribbons D.W. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  23. Nunn W.D., Cheng P.-J., Tang C.T., Tropp B.E. 1977; Phenylethyl alcohol inhibition of .sn-glycerol-3-phosphate acylation in E. coli.. Journal of Bacteriology 130:620–628
    [Google Scholar]
  24. Ohta A., Shibuya I. 1977; Membrane phospholipid synthesis and phenotypic correlation of an E. coli pss mutant. Journal of Bacteriology 132:434–443
    [Google Scholar]
  25. Ohta A., Shibuya I., Maruo B. 1975; E. coli mutants with temperature-sensitive phosphatidyl serine synthetase: genetic analysis. Agricultural and Biological Chemistry 39:2443–2445
    [Google Scholar]
  26. Polacco M.L., Cronan J. E.Jr 1977; Mechanism of the apparent regulation of E. coli unsaturated fatty acid synthesis by exogenous oleic acid. Journal of Biological Chemistry 252:5488–5490
    [Google Scholar]
  27. Radin N.S. 1972; Preparation of lipid extracts. Methods in Enzymology 14:245–254
    [Google Scholar]
  28. Raetz C.R.H. 1976; Phosphatidyl serine synthetase mutants of E. coli: genetic mapping and membrane phospholipid composition. Journal of Biological Chemistry 251:3242–3249
    [Google Scholar]
  29. Raetz C.R.H., Foulds J. 1977; Envelope composition and antibiotic hypersensitivity of E. coli mutants defective in phosphatidyl serine synthetase. Journal of Biological Chemistry 252:5911–5915
    [Google Scholar]
  30. Rooney S.A., Goldfine H., Sweeley C.C. 1972; The identification of trans-2-tetradecenoic acid in hydrolysates of lipid A from E. coli.. Biochimica et biophysica acta 270:289–295
    [Google Scholar]
  31. Sanderson K.E., Macalister T., Costerton J.W., Cheng K.-J. 1974; Permeability of lipopolysaccharide-deficient (rough) mutants of Salmonella typhimurium to antibiotics, lysozyme and other agents. Canadian Journal of Microbiology 20:1135–1145
    [Google Scholar]
  32. Schlenk H., Gellerman J.L. 1960; Esterification of fatty acids with diazomethane on a small scale. Analytical Chemistry 32:1412–1414
    [Google Scholar]
  33. Spencer A., Muller E., Cronan J.E.Jr Gross T.A. 1977; relA gene control of the synthesis of lipid A fatty acyl moieties. Journal of Bacteriology 130:114–117
    [Google Scholar]
  34. Tamaki S., Matsuhashi M. 1973; Increase in sensitivity to antibiotics and lysozyme on deletion of lipopolysaccharide in E. coli strains. Journal of Bacteriology 114:453–454
    [Google Scholar]
  35. Yura T., Wada C. 1968; Phenethyl alcohol resistance in E. coli. I. Resistance of strain C600 and its relation to azide resistance. Genetics 59:177–190
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-113-2-267
Loading
/content/journal/micro/10.1099/00221287-113-2-267
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error