1887

Abstract

Cultures of PAO grown under uninterrupted broad-spectrum light showed different pigmentation from dark-grown cultures. Whereas dark-grown bacteria produced pigments which resulted in blue-purple coloured agar, light-grown organisms produced red coloured plates. Extraction and quantification of pigments showed that both dark- and light-grown cultures produced similar concentrations of pyorubrin (red) and pyoverdin (yellow). In contrast, the concentration of pyocyanin (blue) was substantially reduced under certain lighting conditions. This decrease was dependent on both the light intensity and wavelength and occurred with light in the ultraviolet and violet region of the spectrum. After its release from bacteria, pyocyanin was rapidly and non-reversibly photoinactivated with first-order kinetics to produce colourless photoproduct(s).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-113-2-261
1979-08-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/113/2/mic-113-2-261.html?itemId=/content/journal/micro/10.1099/00221287-113-2-261&mimeType=html&fmt=ahah

References

  1. Elliott R.P. 1958; Some properties of pyoverdine, the water-soluble fluorescent pigment of the pseudomonads. Applied Microbiology 6:241–246
    [Google Scholar]
  2. Jagger J. 1972; Growth delay and photoprotection induced by near ultraviolet light. In Research Progress in Organic-Biological and Medical Chemistry III pp. 383–401 Gallo U., Samtamiria L. Edited by Amsterdam: North Holland.;
    [Google Scholar]
  3. King E.O., Ward M., Raney D. 1954; Two simple media for the demonstration of pyocyanin and fluorescin. Journal of Laboratory and Clinical Medicine 44:301–307
    [Google Scholar]
  4. Leach C.M. 1971; A practical guide to the effects of visible and ultraviolet light on fungi. Methods in Microbiology 4:609–664
    [Google Scholar]
  5. Leonard V. 1924; Pyorubrin, a new pigment produced by Bacilluspyocyaneus.. American Journal of Hygiene 4:404–407
    [Google Scholar]
  6. Palumbo S.A. 1972; Role of iron and sulfur in pigment and slime formation by Pseudomonas aeruginosa.. Journal of Bacteriology 111:430–436
    [Google Scholar]
  7. Propst-Ricciuti C., Kenny K. 1974; The effect of light on the growth of β-lactam antibiotic-producing fungi. Developments in Industrial Microbiology 17:233–240
    [Google Scholar]
  8. Siebert M., Wetherbee P.J., Job D.D. 1975; The effects of light intensity and spectral quality on growth and shoot initiation in tobacco callus. Plant Physiology 56:130–139
    [Google Scholar]
  9. Taylor B.L., Koshland D.E. Jr 1975; Intrinsic and extrinsic light responses of Salmonella typhimurium and Escherichia coli.. Journal of Bacteriology 123:557–569
    [Google Scholar]
  10. Thomas G. 1977; Effects of near-ultraviolet light on microorganisms. Photochemistry and Photobiology 26:669–673
    [Google Scholar]
  11. Zelle M.R., Hollaender A. 1955; Effects of radiation on bacteria. In Radiation Biology. Hollaender A. Edited by New York: McGraw-Hill.;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-113-2-261
Loading
/content/journal/micro/10.1099/00221287-113-2-261
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error