1887

Abstract

Evidence for genetic transformation in is based on the observations that allo-DNA has a specific effect in producing transformants which is abolished by DNAase treatment and that iso-DNA is not effective in transformation. Here, unambiguous evidence for genetic transformation is provided by transfer of a temperature-sensitive inositol requirement from a donor to a recipient strain. Data provided also suggest the role of growth conditions and the involvement of a nuclease gene in the DNA uptake and transformation of .

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/content/journal/micro/10.1099/00221287-113-2-255
1979-08-01
2024-11-08
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References

  1. Avery O.T., McLeod C.M., McCarthy M. 1944; Studies on the chemical nature of substance inducing transformation in pneumococcal types. Journal of Experimental Medicine 79:137–158
    [Google Scholar]
  2. Cosloy S.D., Oishi M. 1973; Genetic transformation in E. coli K12. Proceedings of the National Academy of Sciences of the United States of America 70:84–87
    [Google Scholar]
  3. El-Assouli S., Mishra N.C. 1978; A Neurospora DNA polymerase. Naturwissenschaften 65:63–64
    [Google Scholar]
  4. Forsthoefel A., Mishra N.C. 1977; Biochemical genetics of Neurospora crassa nucleases. Genetics abstr. S20
    [Google Scholar]
  5. Fox A.S., Duggleby F.W., Gelbart W.M., Yoon S.B. 1970; DNA induced transformation in Drosophila: evidence for transformation without integration. Proceedings of the National Academy of Sciences of the United States of America 67:1834–1838
    [Google Scholar]
  6. Hotchkiss R.D. 1976; Concluding remarks. In Modern Trends in Bacterial Transformation and Transfection pp. 321–328 Portales A., Lopez M., Espinosa M. Edited by Amsterdam: North Holland.;
    [Google Scholar]
  7. Ishikawa T., Toh-E A., Uno I., Hsaunuma K. 1969; Isolation and characterization of nuclease mutants in Neurospora crassa.. Genetics 63:75–92
    [Google Scholar]
  8. Lacks S. 1976; Binding and entry of DNA in pneumococcal transformation. In Modern Trends in Bacterial Transformation and Transfection pp. 35–55 Portales A., Lopez M., Espinosa M. Edited by Amsterdam: North Holland.;
    [Google Scholar]
  9. Lacks S., Greenberger B., Neuberger M. 1974; Role of a deoxyribonuclease in the genetic transformation of Diplococcus pneumoniae.. Proceedings of the National Academy of Sciences of the United States of America 71:2305–2309
    [Google Scholar]
  10. Mcbride O.W., Ozer H.L. 1973; Transfer of genetic information by purified metaphase chromosomes. Proceedings of the National Academy of Sciences of the United States of America 70:1258–1262
    [Google Scholar]
  11. Minagawa T., Wagner B., Strauss B. 1959; The nucleic acid content of Neurospora crassa.. Archives of Biochemistry and Biophysics 80:442–445
    [Google Scholar]
  12. Mishra N.C. 1976a; Episome-like behaviour of donor DNA in transformed strains of Neurospora crassa.. Nature; London: 264251–253
    [Google Scholar]
  13. Mishra N.C. 1976b; DNA induced genetic changes in Neurospora crassa.. In Modern Trends in Bacterial Transformation and Transfection pp. 179–183 Portales A., Lopez M., Espinosa M. Edited by Amsterdam: North Holland.;
    [Google Scholar]
  14. Mishra N.C. 1977; Characterization of the new osmotic mutants which originated during genetic transformation in Neurospora crassa.. Genetical Research 29:9–19
    [Google Scholar]
  15. Mishra N.C., Tatum E.L. 1973; Non-Mendelian inheritance of DNA induced inositol independence in Neurospora.. Proceedings of the National Academy of Sciences of the United States of America 70:3875–3879
    [Google Scholar]
  16. Mishra N.C., Szabo G., Tatum E.L. 1973; The role of nucleic acid-induced genetic changes in Neurospora.. In Nucleic Acids in Reproduction and Development pp. 261–273 Niu M.C., Segal S.J. Edited by Amsterdam: North Holland.;
    [Google Scholar]
  17. Oishi M., Irbe R.M. 1976; Circular chromosome and genetic transformation in E. coli.. In Modern Trends in Bacterial Transformation and Transfection pp. 121–134 Portales A., Lopez R., Espinosa M. Edited by Amsterdam: North Holland.;
    [Google Scholar]
  18. Ottolenghi-Nightingale E. 1974; DNA mediated transformation in mammalian cells. In Cell Communication pp. 233–254 Cox R.P. Edited by New York: Wiley.;
    [Google Scholar]
  19. Ruddle R.H., Fournier R.E.K. 1977; Somatic cell genetic analysis of gene transfer in mammalian cells. Brookhaven Symposia in Biology 29:96–104
    [Google Scholar]
  20. Schablik M., Szabolcs M., Kiss A., Aradi J., Zsindeley A., Szabo G. 1977; Conditions of transformation by DNA of Neurospora crassa.. Acta biologica Academiae scientiarum hungaricae 28:273–279
    [Google Scholar]
  21. Vogel H.J. 1964; Evolution of lysin pathways I. Naturalist 98:435–469
    [Google Scholar]
  22. Whitehouse H.L.K. 1965 In Towards an Understanding of the Mechanism of Heredity, 3rd edn. pp. 327–371 New York: St. Martin.;
    [Google Scholar]
  23. Yoshikawa H. 1966; Mutations resulting from the transformation of Bacillus subtilis.. Genetics 54:1201–1214
    [Google Scholar]
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