1887

Abstract

Immunization of mice with a potassium thiocyanate extract of type A (bovine strain) imparted complete protection against an experimental challenge infection with 1·6 × 10 colony-forming units of the homologous strain. Formalin-treated vaccine (one or two doses) was much less effective. Significantly higher bactericidal antibody titres were observed in the sera of mice immunized with the extract compared with those given the vaccine. The bactericidal activity was localized in the IgM and IgG fractions. The extract consisted of protein(s) and carbohydrate(s) and on immunoelectrophoresis displayed at least three antigenically distinct components.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-113-1-37
1979-07-01
2021-05-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/113/1/mic-113-1-37.html?itemId=/content/journal/micro/10.1099/00221287-113-1-37&mimeType=html&fmt=ahah

References

  1. Baba T. 1977; Immunogenic activity of a ribosomal fraction obtained from Pasteurella multocida. Infection and Immunity 15:1–6
    [Google Scholar]
  2. Bains R.V.S. 1955; A preliminary examination of the antigens of Pasteurella multocida type 1. British Veterinary Journal 111:492–498
    [Google Scholar]
  3. Carter G.R. 1967; Pasteurellosis: Pasteurella multocida and Pasteurella hemolytica. Advances in Veterinary Science 11:321–379
    [Google Scholar]
  4. Carter G.R., Rundell S.W. 1975; Identification of type A strain of P. multocida using staphylococcal hyaluronidase. Veterinary Record 96:434
    [Google Scholar]
  5. Collins F.M. 1973; Growth of Pasteurella multocida in vaccinated and normal mice. Infection and Immunity 8:868–875
    [Google Scholar]
  6. Dhanda M.R. 1958; Immunisation of cattle against hemorrhagic septicemia with purified antigens. Indian Veterinary Journal 36:6–8
    [Google Scholar]
  7. Dische Z. 1955 Carbohydrate Estimation in Experimental Immunochemistry, 2nd edn. pp. 526 Kabat E.A., Mayer M.M. Edited by Springfield, Ill.: Charles C. Thomas.;
    [Google Scholar]
  8. Dunnett C.W. 1964; New tables for multiple comparisons with a control. Biometrics 20:482–491
    [Google Scholar]
  9. Gaunt G., Moffat R., Mukkur T.K.S. 1978; Fowl cholera: immunization of chickens with potassium thiocyanate (KSCN) extract of Pasteurella multocida serotype 3. Avian Diseases 21:543–548
    [Google Scholar]
  10. Heddleston K.L., Rebers P.A. 1972; Fowl cholera: cross-immunity induced in turkeys with formalin-killed in vivo-propagated Pasteurella multocida. Avian Diseases 16:578–586
    [Google Scholar]
  11. Heddleston K.L., Gallagher J.E., Rebers P.A. 1972; Fowl cholera: gel diffusion precipitin test for serotyping Pasteurella multocida from avian species. Avian Diseases 16:925–936
    [Google Scholar]
  12. Kenny K.K., Herzberg M. 1968; Antibody response and protection induced by immunization with smooth and rough strains in experimental salmonellosis. Journal of Bacteriology 95:406–417
    [Google Scholar]
  13. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  14. Mukkur T.K.S. 1977; Demonstration of crossprotection between Pasteurella multocida type A and Pasteurella hemolytica, serotype 1. Infection and Immunity 18:583–585
    [Google Scholar]
  15. Mukkur T.K.S., Inman F.P. 1971; Spontaneous reoxidation of the subunits of immunoglobulin M (IgM). Journal of Immunology 107:705–714
    [Google Scholar]
  16. Mukkur T.K.S., Nilakantan P.R. 1972; Preliminary characterization of the crude capsular extracts of Pasteurella multocida, var. Bovine, Type 1 in phase 1. Cornell Veterinarian 42:289–296
    [Google Scholar]
  17. Prince G.H., Smith J.E. 1966; Antigenic studies on Pasteurella multocida using immunodiffusion technics. I. Identification and nomenclature of the soluble antigens of a bovine hemorrhagic septicemia strain. Journal of Comparative Pathology 76:303–314
    [Google Scholar]
  18. Rebers P.A., Heddleston K.L. 1974; Immunological comparison of Westphal-type lipopolysaccharides and free endotoxins from an encapsulated and a non-encapsulated avian strain of Pasteurella multocida. American Journal of Veterinary Research 35:555–560
    [Google Scholar]
  19. Reed L.H., Muench H. 1938; A simple method for estimating fifty percent end-points. American Journal of Hygiene 27:493
    [Google Scholar]
  20. Scheidegger J.J. 1955; Une micro-méthode de l’immunoelectrophorèse. International Archives of Allergy and Applied Immunology 7:103–110
    [Google Scholar]
  21. Schwab J.H. 1975; Suppression of the immune response by microorganisms. Bacteriological Reviews 39:121–143
    [Google Scholar]
  22. Thomson R.G., Gilka F. 1974; A brief review of pulmonary clearance of bacterial aerosols emphasizing aspects of relevance to veterinary medicine. Canadian Veterinary Journal 15:99–107
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-113-1-37
Loading
/content/journal/micro/10.1099/00221287-113-1-37
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error