sp. NCMB 1314 produces, especially under Mg-limited growth conditions, a factor that liberates a cholinesterase from plaice muscle. Just after the onset of Mg limitation in a batch culture, there is a period of rapid production of the factor. Changes also occur in the morphology, ATP content and gross chemical composition of the bacteria. The protein and carbohydrate contents are raised by 60% and 100%, respectively, while the RNA and ATP contents are reduced to 70% and 40% of the original values. Increased amounts of carbohydrate found outside the cells after Mg limitation at least partly correlated with extracellular slime observed in electron micrographs.


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