1887

Abstract

SUMMARY: Wall fragments were prepared from two strains of and from , and their contents of readily extractable lipid, pronase-digestible protein and lipo-polysaccharide were measured. Lipopolysaccharide extracted from , although biologically active, contained no detectable 2-keto-3-deoxyoctonic acid, but contained phosphate, rhamnose, glucose, heptose and hexosamine in concentrations comparable to those found in .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-112-2-397
1979-06-01
2021-05-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/112/2/mic-112-2-397.html?itemId=/content/journal/micro/10.1099/00221287-112-2-397&mimeType=html&fmt=ahah

References

  1. Adair F. W., Geftic S. G., Gelzer J. 1971; Resistance of Pseudomonas to quaternary ammonium compounds. II. Cross resistance characteristics of a mutant of Pseudomonas aeruginosa . Applied Microbiology 21:1058–1063
    [Google Scholar]
  2. Adams G. A., Quadling C., Yeguchi M., Tornabene T. G. 1970; The chemical composition of the cell-wall lipopolysaccharides from Moraxella duplex and Micrococcus calco-aceticus . Canadian Journal of Microbiology 16:1–8
    [Google Scholar]
  3. Ashwell G. 1957; Colorimetric analysis of sugars. Methods in Enzymology 3:73–105
    [Google Scholar]
  4. Ballard R. W., Palleroni N. J., Doudoroff M., Stanier R. Y., Mandel M. 1970; Taxonomy of the aerobic pseudomonads: Pseudomonas cepacia, P. marginata, P. alliicola, and P. caryophylli. Journal of General Microbiology 60:199–214
    [Google Scholar]
  5. Bassett P. C. J., Dickson J. A. S., Hunt G. H. 1973; Infection of Holter-valve by Pseudomonas- contaminated chlorhexidine. Lancet i:1263–1264
    [Google Scholar]
  6. Brown M. R. W. 1975; The role of the cell envelope in resistance. In Resistance of Pseudomonas aeruginosa pp 71–107 Edited by Brown M. R. W. London: John Wiley;
    [Google Scholar]
  7. Buttke T. M., Ingram L. O. 1975; Comparison of lipopolysaccharides from Agmenellum quadruplicatum to Escherichia coli and Salmonella typhimurium by using thin layer chromatography. Journal of Bacteriology 124:1566–1573
    [Google Scholar]
  8. Chester I. R., Murray R. G. E. 1975; Analysis of the cell wall and lipopolysaccharide of Spirillum serpens . Journal of Bacteriology 124:1168–1176
    [Google Scholar]
  9. Gelbart S. M., Reinhardt G. R., Greenlee H. B. 1976; Pseudomonas cepacia strains isolated from water reservoirs of unheated nebulizers. Journal of Clinical Microbiology 3:62–66
    [Google Scholar]
  10. Ghuysen J.-M., Tipper D. J., Strominger J. L. 1966; Enzymes that degrade bacterial cell walls. Methods in Enzymology 8:685–699
    [Google Scholar]
  11. Hamilton W. A. 1971; Membrane-active antibacterial compounds. In Inhibition and Destruction of the Microbial Cell pp 77–93 Edited by Hugo W. B. New York: Academic Press;
    [Google Scholar]
  12. Heymann H., Manniello J. M., Barkulis S. S. 1967; Structure of streptococcal cell walls. V. Phosphate esters in the walls of group A Streptococcus pyogenes . Biochemical and Biophysical Research Communications 26:486–491
    [Google Scholar]
  13. Hickman J., Ashwell G. 1966; Isolation of a bacterial lipopolysaccharide containing 3-acet- amido-3,6-dideoxy-d-galactose and d-rhamnose. Journal of Biological Chemistry 241:1424–1428
    [Google Scholar]
  14. Hofstad T., Kristofferson T. 1970; Chemicalcharacteristics of endotoxin from Bacteroides fragilis NCTC 9343. Journal of General Microbiology 61:15–19
    [Google Scholar]
  15. Hough L., Jones J. K. N., Wadman W. H. 1950; Quantitative analysis of mixtures of sugars by the method of partition chromatography. V. Improved methods for the separation and detection of the sugars and their methylated derivatives on the paper chromatogram. Journal of the Chemical Society1702–1706
    [Google Scholar]
  16. Hurlbert R. E., Weckesser J., Mayer H., Fromme I. 1976; Isolation and characterization of the lipopolysaccharide of Chromatium vinosum . European Journal of Biochemistry 68:365–371
    [Google Scholar]
  17. Janda J., Work E. 1971; A colorimetric estimation of lipopolysaccharides. FEBS Letters 16:343–345
    [Google Scholar]
  18. Karkhanis Y. D., Zeltner J. Y., Jackson J. J., Carlo D. J. 1978; A new microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of gram-negative bacteria. Analytical Biochemistry 85:595–601
    [Google Scholar]
  19. Kaspar D. L. 1976; Chemical and biological characterization of the lipopolysaccharide of Bacteroides fragilis subspecies fragilis . Journal of Infectious Diseases 134:59–66
    [Google Scholar]
  20. Lüderitz O., Galanos C., Lehmann V., Nurminen M., Rietschel T. T., Rosenfelder G., Simon M., Westphal O. 1973; Lipid A: chemical structure and biological activity. In Bacterial Lipopolysaccharides pp 9–21 Edited by Kass E. H., Wolff S. M. Chicago: University of Chicago Press;
    [Google Scholar]
  21. Osborn M. J. 1963; Studies on the gram-negative wall. 1. Evidence for the role of 2-keto-3-deoxy- octonate in the lipopolysaccharide of Salmonella typhimurium . Proceedings of the National Academy of Sciences of the United States of America 50:499–506
    [Google Scholar]
  22. Trevelyan W. E., Procter D. P., Harrison J. S. 1950; Detection of sugars on paper chromatograms. Nature, London 166:444–445
    [Google Scholar]
  23. United States Pharmacopeia Convention Inc 1974; Pyrogen test. In United States Pharmacopeia XIX p 613 Easton, Pennsylvania: Mack Publishing Co;
    [Google Scholar]
  24. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. Methods in Carbohydrate Chemistry 5:83–91
    [Google Scholar]
  25. Wright B. E., Rebers P. A. 1972; Procedure for determining heptose and hexose in lipopolysaccharides: modification of the cysteine-sulfuric acid method. Analytical Biochemistry 49:307–319
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-112-2-397
Loading
/content/journal/micro/10.1099/00221287-112-2-397
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error