The Control of the Synthesis of Isocitrate Lyase in a Thermophilic Bacillus Free

Abstract

SUMMARY: From a strain of , devoid of active pyruvate carboxylase, a mutant (NG-15) was selected that grew on acetate in the presence of glucose. This mutant differed from its parent organism in possessing high activities of isocitrate lyase when grown on all carbon sources tested except nutrient broth, in possessing unusually low activities of NADP-dependent isocitrate dehydrogenase and in containing increased amounts of isocitrate. Revertants of mutant NG-15 which regained the ability to synthesize active pyruvate carboxylase also synthesized isocitrate lyase and isocitrate dehydrogenase to the same extent as the wild-type strain. These results suggest that the regulatory mechanism for the synthesis of isocitrate lyase in the thermophile may be different from that in mesophilic bacilli.

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1979-06-01
2024-03-28
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References

  1. Ashworth J. M., Kornberg H. L. 1964; The role of isocitrate lyase in Escherichia coli . Biochimica et biophysica acta 89:383–384
    [Google Scholar]
  2. Bennett P. M., Holms W. H. 1975; Reversible inactivation of the isocitrate dehydrogenase of Escherichia coli ML308 during growth on acetate. Journal of General Microbiology 87:37–51
    [Google Scholar]
  3. Cazzulo J. J., Sundaram T. K., Kornberg H. L. 1970; Properties and regulation of pyruvate carboxylase from Bacillus stearothermophilus . Proceedings of the Royal Society B176:1–19
    [Google Scholar]
  4. Chell R. M., Sundaram T. K. 1975; Mutants of Bacillus stearothermophilus de-repressed for isocitrate lyase and malate synthase. Biochemical Society Transactions 3:306–309
    [Google Scholar]
  5. Cleland W. W., Thompson V. W., Barden R. E. 1969; Isocitrate dehydrogenase (TPN-specific) from pig heart. Methods in Enzymology 13:30–33
    [Google Scholar]
  6. Dixon G. H., Kornberg H. L. 1959; Assay methods for key enzymes of the glyoxylate cycle. Biochemical Journal 72:3p
    [Google Scholar]
  7. Epstein I., Grossowicz N. 1969; Prototrophic thermophilic Bacillus: isolation, properties and kinetics of growth. Journal of Bacteriology 99:414–417
    [Google Scholar]
  8. Flavell R. B., Woodward D. O. 1971; Metabolic role, regulation of synthesis, cellular location and genetic control of the glyoxylate cycle enzymes in Neurospora crassa . Journal of Bacteriology 105:200–210
    [Google Scholar]
  9. Griffiths M. W. 1974 The biochemistry of thermophilic micro-organisms. Ph. D. thesis University of Leicester;
    [Google Scholar]
  10. Griffiths M. W., Sundaram T. K. 1973; Isocitrate lyase from a thermophilic Bacillus: effect of salts on enzyme activity. Journal of Bacteriology 116:1160–1169
    [Google Scholar]
  11. Harris P., Kornberg H. L. 1972; The uptake of glucose by a thermophilic Bacillus sp. Proceedings of the Royal Society B182:159–170
    [Google Scholar]
  12. Herman N. J., Bell E. J. 1970; Metabolic control in Acinetobacter sp. I. Effect of C4 versus C2 and C3 substrates on isocitrate lyase synthesis. Canadian Journal of Microbiology 16:769–774
    [Google Scholar]
  13. Higgins I. J., Sariaslani F. S. 1973; Control of isocitrate lyase in Nocardia salmonicolor (NCIB 9701). Proceedings of the Society for General Microbiology 1:21–22
    [Google Scholar]
  14. John P. C. L., Syrett P. J. 1968; The inhibition, by intermediary metabolites, of isocitrate lyase from Chlorella pyrenoidosa . Biochemical Journal 110:481–484
    [Google Scholar]
  15. Klotzsch H. & Bergmeyer H. -U. 1965; Glyoxylic acid. In Methods of Enzymatic Analysis pp 300–302 Edited by Bergmeyer H. -U. New York & London: Verlag Chemie/Academic Press;
    [Google Scholar]
  16. Kornberg H. L. 1965; Control of biosynthesis from C2 compounds. Colloques internationaux du Centre national de la recherche scientifique 124:193–207
    [Google Scholar]
  17. Kornberg H. L. 1966; The role and control of the glyoxylate cycle in Escherichia coli . Biochemical Journal 99:1–11
    [Google Scholar]
  18. Kornberg H. L., Krebs H. A. 1957; Synthesis of cell constituents from C2-units by a modified tricarboxylic acid cycle. Nature, London 179:988–991
    [Google Scholar]
  19. Kornberg H. L., Madsen N. B. 1957; Synthesis of C4-dicarboxylic acids from acetate by a ‘glyoxylate bypass’ of the tricarboxylic acid cycle. Biochimica et biophysica acta 24:651–653
    [Google Scholar]
  20. Kornberg H. L., Madsen N. B. 1958; The metabolism of C2-compounds in micro-organisms. 3. Synthesis of malate from acetate via the glyoxylate cycle. Biochemical Journal 68:549–557
    [Google Scholar]
  21. Kornberg H. L., Smith J. 1969; Genetic control of hexose phosphate uptake by Escherichia coli . Nature, London 224:1261–1262
    [Google Scholar]
  22. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  23. Lowry O. H., Carter J., Ward J. B., Glaser L. 1971; The effect of carbon and nitrogen sources on the level of metabolic intermediates in Escherichia coli . Journal of Biological Chemistry 246:6511–6521
    [Google Scholar]
  24. Monod J. 1956; Remarks on the mechanism of enzyme induction. In Enzymes: Units of Biological Structure and Function pp 7–28 Edited by Gaebler O. H. New York: Academic Press;
    [Google Scholar]
  25. Racker E. 1950; Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids. Biochimica et biophysica acta 4:211–214
    [Google Scholar]
  26. Rutberg B., Hoch J. A. 1970; Citric acid cycle: gene–enzyme relationships in Bacillus subtilis . Journal of Bacteriology 104:826–833
    [Google Scholar]
  27. Sundaram T. K. 1973; Physiological role of pyruvate carboxylase in a thermophilic Bacillus . Journal of Bacteriology 113:549–557
    [Google Scholar]
  28. Sundaram T. K., Kornberg H. L. 1969; Kinetic parameters of mesophilic and thermophilic isocitrate lyase. Journal of General Microbiology 55:xvii
    [Google Scholar]
  29. Sundaram T. K., Cazzulo J. J., Kornberg H. L. 1969; Anaplerotic CO2 fixation in mesophilic and thermophilic bacilli. Biochimica et biophysica acta 192:355–357
    [Google Scholar]
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