1887

Abstract

SUMMARY: In the genetically unstable, protease-overproducing ‘medusa’ (M) strains of , segregation of stable, wild-type-like B cells occurred mainly during sporulation. After the end of the exponential growth phase, a small fraction of M cells sporulated quickly and formed M spores, while the majority of the cells, after a ‘critical period’, gave rise to B segregants which sporulated after a delay. Segregation occurred without cell division. Delayed sporulation, segregation and protease overproduction are related. Similar but more complex results were obtained with the highly unstable TD strains. Sporulation and the kinetics of protease overproduction were also followed in several stable segregants. Depending on the strain, either the rate of protease production or both the rate and time course were affected. The results are interpreted in terms of sequential activation and de-activation of sporulation genes. The production of the alkaline and the neutral proteases was, in general, under common genetic control. In some strains α-amylase was also overproduced.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-112-2-283
1979-06-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/112/2/mic-112-2-283.html?itemId=/content/journal/micro/10.1099/00221287-112-2-283&mimeType=html&fmt=ahah

References

  1. Aubert J. P., Ryter A., Schaeffer P. 1968; Comportement de l’ADN des bactéries et des spores au cours d’un cycle sporal chez B . subtilis. Annales de l’Institut Pasteur 115:989–1007
    [Google Scholar]
  2. Aubert J. P., Ryter A., Schaeffer P. 1969; Fate of spore deoxyribonucleic acid in sporulating Bacillus megaterium . In Spores IV pp 148–158 Edited by Campbell L. L. Bethesda, Md: American Society for Microbiology;
    [Google Scholar]
  3. Balassa G. 1969; Biochemical genetics of bacterial sporulation. I. Unidirectional pleiotropic interactions among genes controlling sporulation in Bacillus subtilis . Molecular and General Genetics 104:73–103
    [Google Scholar]
  4. Balassa G., Dod B., Zucca J. 1978; Overproduction of sporulation-associated extracellular protease in Bacillus subtilis mutants. In Spores VI pp 279–281 Edited by Gerhardt Ph., Costilow Ph., Sadoff H. L. Washington D. C.: American Society for Microbiology;
    [Google Scholar]
  5. Dod B., Balassa G. 1978; Spore control (Sco) mutations in Bacillus subtilis. III. Regulation of extracellular protease synthesis in the spore control mutations ScoC . Molecular and General Genetics 163:57–63
    [Google Scholar]
  6. Dod B., Balassa G., Raulet E., Jeannoda V. 1978; Spore control (Sco) mutations in Bacillus subtilis. II. Sporulation and the production of extracellular proteases and amylase by Sco mutants. Molecular and General Genetics 163:45–56
    [Google Scholar]
  7. Doi R. H. 1972; Role of proteases in sporulation. Current Topics in Cellular Regulation 6:1–20
    [Google Scholar]
  8. Milhaud P., Balassa G., Zucca J. 1978; Spore control (Sco) mutations in Bacillus subtilis. I. Selection and genetic mapping of Sco mutants. Molecular and General Genetics 163:35–44
    [Google Scholar]
  9. Millet J. 1970; Characterization of proteinases excreted by B. subtilis Marburg strain during sporulation. Journal of Applied Bacteriology 33:207–219
    [Google Scholar]
  10. Ryter A., Aubert J. P. 1969; Étude autoradiographique de la synthèse de l’ADN au cours de la sporulation de Bacillus subtilis . Annales de l’Institut Pasteur 117:601–611
    [Google Scholar]
  11. Schaeffer P. 1969; Sporulation and the production of antibiotics, exoenzymes and exotoxins. Bacteriological Reviews 33:48–71
    [Google Scholar]
  12. Sousa J. C. F., Silva M. T., Balassa G. 1978; Spore control (Sco) mutations in Bacillus subtilis. V. Electron microscope study of delayed morphogenesis. Molecular and General Genetics 163:285–291
    [Google Scholar]
  13. Zucca J., Balassa G. 1979; Genetic instability of sporulation-associated characters in a Bacillus subtilis mutant: analysis of the segregation pattern and genetic studies. Journal of General Microbiology 112:269–281
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-112-2-283
Loading
/content/journal/micro/10.1099/00221287-112-2-283
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error