1887

Abstract

When polymorphonuclear leucocytes (PMN) elicited in mice were infected with or , phagosome–lysosome fusion occurred with both species. This contrasts with the situation in macrophages where phagosome–lysosome fusion is inhibited by but not . No evidence was found for killing of or when the bacteria were isolated from PMN and their viability tested in cell-free medium or macrophages.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-112-1-185
1979-05-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/112/1/mic-112-1-185.html?itemId=/content/journal/micro/10.1099/00221287-112-1-185&mimeType=html&fmt=ahah

References

  1. Adams D. O. 1975; The structure of mononuclear phagocytes differentiating in vivo II. The effect of Mycobacterium tuberculosis. American Journal of Pathology 80:101–116
    [Google Scholar]
  2. Alexander J., Vickerman K. 1975; Fusion of host cell secondary lysosomes with parasitoPhorous vacuoles of Leishmania mexicana infected macrophages. Journal of Protozoology 22:502–508
    [Google Scholar]
  3. Armstrong J. A., Hart P. D. 1971; Response of cultured macrophages to Mycobacterium tuberculosis, with observations on fusion of lysosomes with phagosomes. Journal of ExperiMental Medicine 134:713–740
    [Google Scholar]
  4. Armstrong J. A., Hart P. D. 1975; Phagosome–lysosome interactions in cultured macrophages infected with virulent tubercle bacilli. Reversal of the usual non-fusion pattern and observations on bacterial survival. Journal of Experimental Medicine 142:1–16
    [Google Scholar]
  5. Brown C. A., Draper P., Hart P. D. 1969; Mycobacteria and lysosomes: a paradox. Nature, London 221:658–660
    [Google Scholar]
  6. Closs O., Haugan O. A. 1975; Experimental murine leprosy. 3. Early local reaction to Mycobacterium lepraemurium in C3H And C57 Bl mice. Acta pathologica et microbiologica scandinavica 83:51–58
    [Google Scholar]
  7. Evans M. J., Levy L. 1972; Ultrastructural changes in cells of the mouse footpad infected with Mycobacterium leprae. Infection and Immunity 5:238–247
    [Google Scholar]
  8. Godal T., Rees R. J. W., Lamvic J. O. 1970; Lymphocyte mediated modification of blood-derived macrophage function in vitro inhibition of growth of intracellular mycobacteria with lymphokines. Clinical and Experimental Immunology 8:625–637
    [Google Scholar]
  9. Hanks J. H., Evans B. 1940; The action of serum, cells and blood on acid fast bacteria in vitro. I. Absence of in vitro bactericidal power against tubercle bacilli or Timothy bacilli in the serum leucocyte mixtures or the blood of normal and immunized rabbits. American Review of Tuberculosis and Pulmonary Diseases 41:605–644
    [Google Scholar]
  10. Hart P. D., Rees R. J. W. 1960; Effect of macrocyclon in acute and chronic pulmonary tuberculosis infection in mice as shown by viable and total bacterial counts. British Journal of Experimental Pathology 141:414–421
    [Google Scholar]
  11. Hart P. D., Valentine R. C. 1963; Growth (without multiplication) of Mycobacterium lepraemurium in cell free medium. Journal of General Microbiology 32:43–53
    [Google Scholar]
  12. Hart P. D., Armstrong J. A., Brown C. A., Draper P. 1972; Ultrastructural study on the behaviour of macrophages towards parasitic mycobacteria. Infection and Immunity 5:803–807
    [Google Scholar]
  13. Hirsch J. G., Fedorko M. E. 1968; Ultrastructure of human leukocytes after simultaneous fixation with glutaraldehyde and osmium tetroxide and postfixation in uranyl acetate. Journal of Cellular Biology 38:615–627
    [Google Scholar]
  14. Jones T. C., Hirsch J. G. 1972; The interaction between Toxoplasma gondii and mammalian cells II. The absence of lysosomal fusion with phagocytic vacuoles containing living organisms. Journal of Experimental Medicine 136:1173–1194
    [Google Scholar]
  15. Kress Y., Blooim B. R., Wittner M., Rowen A., Tanowitz H. 1975; Resistance of Trypanosoma cruzi to killing by macrophages. Nature, London 251:394–396
    [Google Scholar]
  16. Noguiera N., Conn Z. 1976; Trypanosoma cruzi: mechanism of entry and intracellular fate in mammalian cells. Journal of Experimental Medicine 143:1402–1420
    [Google Scholar]
  17. Sharp K. 1973; Mycobacterium microti (Vole Bacillus): a method for viable counts within 21 days of culture. Applied Microbiology 25:1023–1024
    [Google Scholar]
  18. Ungar J., Muggleton P. W., Dudley J. A. R., Griffiths M. L. 1962; Preparation and properties of a freeze-dried BCG vaccine of increased stability. British Medical Journal ii:1086–1091
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-112-1-185
Loading
/content/journal/micro/10.1099/00221287-112-1-185
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error