1887

Abstract

The soluble fraction extracted from A19 (Hfr, ) during early and late times of phenotypic and genotypic induced relaxed control have been examined for the possible accumulation of ribosomal proteins (r-proteins) and rRNA species during this time of unbalanced macromolecular synthesis. Ribosomal proteins and rRNA species were not found to accumulate within the soluble fraction at any time during this period of relaxed control; even after the typical rRNA accumulation had ceased, r-proteins did not accumulate. It is concluded, from these and related observations, that the r-proteins and rRNA species known to be produced during relaxation must immediately associate to form the unusual ribonucleoprotein particles (e.g. ‘relaxed particles’ and ‘chloramphenicol particles’) characteristic of periods of relaxed control. Since r-proteins do not accumulate even when net RNA accumulation halts, it appears that some elements of the normal, basic co-ordination between rRNA and r-protein synthesis/stability persist even during relaxed control.

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/content/journal/micro/10.1099/00221287-112-1-149
1979-05-01
2021-05-13
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References

  1. Adesnik M., Levinthal C. 1969; Synthesis and maturation of ribosomal RNA in Escherichia coli.. Journal of Molecular Biology 46:281–303
    [Google Scholar]
  2. Chang B., Irr J. 1973; Maturation of ribosomal RNA in stringent and relaxed bacteria. Nature New Biology 243:35–37
    [Google Scholar]
  3. Dean J. R., Sykes J. 1974; The role of ribonuclease II in the maturation of precursor 16S ribosomal ribonucleic acid in Escherichia coli. Biochemical Journal 140:443–450
    [Google Scholar]
  4. Dennis P. P. 1976; Effects of chloramphenicol on the transcriptional activities of ribosomal RNA and ribosomal protein genes in Escherichia coli.. Journal of Molecular Biology 108:535–546
    [Google Scholar]
  5. Dennis P. P., Nomura M. 1974; Stringent control of ribosomal protein gene expression in Escherichia coli.. Proceedings of the National Academy of Sciences of the United States of America 71:3819–3823
    [Google Scholar]
  6. Dennis P. P., Nomura M. 1975a; Stringent control of the transcriptional activities of ribosomal protein genes in Escherichia coli.. Nature, London 255:460–465
    [Google Scholar]
  7. Dennis P. P., Nomura M. 1975b; Regulation of the expression of ribosomal protein genes in Escherichia coli.. Journal of Molecular Biology 97:61–76
    [Google Scholar]
  8. Gausing K. 1974; Ribosomal protein in Escherichia coli: rate of synthesis and pool size at different growth rates. Molecular and General Genetics 129:61–75
    [Google Scholar]
  9. Goodman D. 1970; Ribosomal protein synthesis during amino acid starvation and chloramphenicol treatment. Journal of Molecular Biology 51:491–499
    [Google Scholar]
  10. Goodman D., Manor H., Rombauts W. 1969; Ribosomal protein synthesis during and after amino acid starvation in relaxed and stringent bacteria. Journal of Molecular Biology 40:247–260
    [Google Scholar]
  11. Hardy S. J., Kurland G. C., Voynow P., Mora G. 1969; The ribosomal proteins of Escherichia coli: purification of the 30S proteins. Biochemistry 8:2897–2905
    [Google Scholar]
  12. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  13. Kurland G. C. 1960; Molecular characterisation of ribonucleic acid from Escherichia coli ribosomes. Journal of Molecular Biology 2:83–91
    [Google Scholar]
  14. Stickland L. H. 1951; The determination of small quantities of bacteria by means of the biuret reaction. Journal of General Microbiology. 5:698–703
    [Google Scholar]
  15. Subramanian A. R. 1974; Sensitive separation procedure for Escherichia coli ribosomal proteins and the resolution of high molecular weight components. European Journal of Biochemistry 45:541–546
    [Google Scholar]
  16. Sykes J., Metcalf E., Pickering J. D. 1977 a; The nature of the proteins in ‘chloramphenicol particles’ from Escherichia coli A19 (Hfr rel met rns). Journal of General Microbiology 98:1–16
    [Google Scholar]
  17. Sykes J., Metcalf E., Pickering J. D. 1977b; The nature of the proteins present in the ‘relaxed particles’ from methionine-starved Escherichia coli A19 (Hfr rel met rns). Journal of General Microbiology 98:17–27
    [Google Scholar]
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