1887

Abstract

, an extremely halophilic bacterium, lacks a normal peptidoglycan cell wall. Nevertheless, bacitracin, a known inhibitor of peptidoglycan synthesis, has been shown to completely suppress the growth of this organism (Mescher & Strominger, 1975) In an attempt to clarify this observation, the effect of bacitracin on DNA, RNA, protein and lipid biosynthesis in was monitored. Lipid carbohydrate and lipid phosphate were the most affected. These observations were confirmed by uptake studies of [glycerol into the membrane lipids. The possibility that bacitracin acts against through inhibition of dephosphorylation of the isoprenoid pyrophosphate precursors of this organism's diether lipids is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-111-2-423
1979-04-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/111/2/mic-111-2-423.html?itemId=/content/journal/micro/10.1099/00221287-111-2-423&mimeType=html&fmt=ahah

References

  1. AMES B.N., DUBIN D.T. 1960; The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acids.. Journal of Biological Chemistry 235:769–775
    [Google Scholar]
  2. BLIGH E.G., DYER W.J. 1959; A rapid method of total lipid extraction and purification.. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  3. CERIOTTI G. 1952; A micro-chemical determination of deoxyribonucleic acid.. Journal of Biological 198:297–303
    [Google Scholar]
  4. DU‘BOIS M., GILLES K.A., HAMILTON J.K., REBERS P. A., SMITH F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  5. HALLIBURTON I.W., THOMSON R.Y. 1965; Chemical aspects of compensatory renal hypertrophy. Cancer Research 25:1882
    [Google Scholar]
  6. HORNE D., HAKENBECK R., TOMASZ A. 1977; Secretion of lipids induced by inhibition of peptidoglycan synthesis in streptococci.. Journal 0, Bacterio logy 132:704–717
    [Google Scholar]
  7. KATES M. Ether-linked lipids in extremely halophilic bacteria.. In Snyder. F. Ether Lipids, Chemistry and Biology pp. New York: Academic Press.;351–398 1972
    [Google Scholar]
  8. KATES M., YENGOYAN L.S., SASTRY P.S. 1965; A diether analog of phosphatidylglycerophosphate in Halobacterium cutirubrum. Biochimicaet biophysica acta 98:252–268
    [Google Scholar]
  9. KATES M., WASSEF M.I., PUGH E.L. 1970; Origin of the glycerol moieties in the glycerol diether lipids of Halobacterium cutirubrum. Biochimica et biophysica acta 202:206–208
    [Google Scholar]
  10. KUSHNER D.J., BAYLEY S.T., BORING J., KATES M., GIBBONS N.E. 1964; Morphological and chemical properties of cell envelopes of the extreme halophile, Halobacterium cutirubrum.. Canadian Journal of Microbiology 10:483–497
    [Google Scholar]
  11. LOWRY O.H., ROSEBROUGH N.J., FARR A.L., RANDALL R.J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  12. MESCHER M.F., STROMINGER J.L. 1975; Bacitracin induces sphere formation in Halobacterium species which lack a wall peptidoglycan. Journal of General Microbiology 89:375–378
    [Google Scholar]
  13. MESCHER M.F., STROMINGER J.L. 1976; Purification and characterization of a prokaryotic glycoprotein from the cell envelope of Halobacterium salinarium.. Journal of BiologicalChemistry 251:2005–2014
    [Google Scholar]
  14. MESCHER M.F., STROMINGER J.L., WATSON S.W. 1974; Protein and carbohydrate composition of the cell envelope of Halobacterium salinarium.. Journal of Bacteriology 120:945–954
    [Google Scholar]
  15. PARK J.T. 1961; Inhibition of synthesis of bacterial mucopeptide or protein by certain antibiotics and its possible significance for microbiology and medicine.. Antimicrobial Agents Annual 338
    [Google Scholar]
  16. SEHGAL S.N., GIBBONS N.E. 1960; Effect of some metal ions on the growth of Halobacterium cutirubrum.. Canadian Journal of Microbiology 6:156–169
    [Google Scholar]
  17. SIEWERT G., STROMINGER J.L. 1967; Bacitracin, an inhibitor of the dephosphorylation of lipid pyrophosphate, an intermediate in the biosynthesis of bacterial cell walls.. Proceedings of the National Academy of Sciences of the United States of America 57:767–773
    [Google Scholar]
  18. SMITH J.L., WEINBERG E.D. 1962; Mechanisms of antibacterial action of bacitracin.. Journal of General Microbiology 28:559–569
    [Google Scholar]
  19. STORM B.R., STROMINGER J.L. 1973; Complex formation between bacitracin peptides and isoprenyl pyrophosphates.. Journal of Biological Chemistry 248:3940–3945
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-111-2-423
Loading
/content/journal/micro/10.1099/00221287-111-2-423
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error