Summary: Populations of with plasma membranes enriched in phosphatidyl-ethanolamine lost viability and released cations at a greater rate when suspended in buffered sodium dodecyl sulphate than did populations with membranes enriched in phosphatidylcholine. However, when suspended in buffered sorbitol (1.2 M) containing sodium dodecyl sulphate, sphaeroplasts from organisms with phosphatidylcholine-enriched membranes released cations faster than did sphaeroplasts with membranes enriched in phosphatidylethanolamine. Liposomes prepared in potassium chloride from mixtures of phospholipids from organisms enriched in phosphatidylcholine or phosphatidylethanol-amine lost potassium ions at the same rate when suspensions were supplemented with sodium dodecyl sulphate. Organisms enriched in phosphatidylcholine or phosphatidyl-ethanolamine did not differ in appearance in scanning electron micrographs, in electrophoretic mobilities over the pH range 2 to 9, in the ease with which they were converted into sphaero-plasts by β-glucanase, or in permeability to a range of polyethylene glycols. Walls from phosphatidylcholine- and phosphatidylethanolamine-enriched organisms had the same contents of β-glucans, α-mannan and protein.


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