1887

Abstract

Methyl viologen (10 μM) markedly inhibited acetylene reduction (nitrogen fixation) by old but not young cultures of sp. LB795, apparently by causing the alga to produce HO. HO inhibited acetylene reduction when added to cultures at concentrations greater than 10 μM. As catalase (EC 1.11.1.6) is not present in sp. LB795, HO is probably removed by a non-enzymic reaction with ascorbate and also by an enzyme-catalysed reaction with glutathione. Enzymes catalysing the decomposition of HO, were most active in young cells which were therefore better able than old cells to metabolize HO produced in the presence of methyl viologen. The maximum activities of these enzymes coincided with maximum nitrogenase activity during the growth of batch cultures, and may provide a protective mechanism for nitrogenase.

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1979-04-01
2024-04-25
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References

  1. BAILEY J. L. 1962 Techniques in Protein Chemistry p. 293: Amsterdam: Elsevier Publishing Co;
    [Google Scholar]
  2. BOTHE H., TENNIGKEIT J., EISBRENNER G. 1977; The utilisation of molecular hydrogen by the blue-green alga Anabaena cylindrica . Archives ofMicrobiology 114:43–49
    [Google Scholar]
  3. CARPENTER. E. J., PRICE IV C. C. 1976; Marine Oscillatoria (Trichodesmium) : explanation for aerobic nitrogen fixation without heterocysts.. Science 191:1278–1280
    [Google Scholar]
  4. CHANCE B, MAEHLY A. C. 1955; Assay of catalases and peroxidases. Methods in Enzym-ology 2:764–775
    [Google Scholar]
  5. CHUA N.-H. 1971; The methyl viologen-catalysed Mehler reaction and catalase activity in blue-green algae and Chlamydomonas reinhardii . Biochemistryet biophysica acta 245:277–287
    [Google Scholar]
  6. FARRINGTON J. A, EBERT M, LAND E. J., FLETCHER K. 1973; Bipyridylium quaternary salts and related compounds. V. Pulse radiolysis studies of the reaction of paraquat radical with oxygen. Implications for the mode of action of bipyridyl herbicides. Biochimica et biophysica acta 314:372–381
    [Google Scholar]
  7. FLOHE L, MENZEL A. 1971; The influence of glutathione upon light-induced high-amplitude swelling and lipid peroxide formation of spinach chloroplasts. Plant and Cell Physiology 12:325–333
    [Google Scholar]
  8. FOYER C. H., HALLIWELL B. 1976; The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133:21–25
    [Google Scholar]
  9. GALLON J. R, LARUE T. A, KURZ W. G. W. 1972; Characteristics of nitrogenase activity in broken cell preparations of the blue-green alga Gloeocapsa sp. LB795. Canadian Journal of Micro-biology 18:327–332
    [Google Scholar]
  10. GALLON J. R, LARUE T. A, KURZ W. G. W. 1974; Photosynthesis and nitrogenase activity in the blue-green, alga Gloeocapsa . Canadian Journal of Microbiology 20:1633–1637
    [Google Scholar]
  11. GALLON J. R, KURZ W. G. W., LARUE T. A. The physiology of nitrogen fixation by a Gloeocapsa sp.. in Stewart W. D. P. Nitrogen Fixation by Free-living Microorganisms,International Biological Programme PP Cambridge: Cambridge University Press; vol. 6:159–173 1975
    [Google Scholar]
  12. GALLON J. R, UL-HAQUE M. I., CHAPLIN A. E. 1978; Fluoroacetate metabolism in Gloeocapsa sp. LB795 and its relationship to acetylene reduction (nitrogen fixation). Journal of General Microbiology 106:329–336
    [Google Scholar]
  13. GRODEN D, BECK E. 1977; Characterisationof a membrane-bound ascorbate-specific peroxidase from spinach chloroplasts. in Coombs J., Hall D. O. Abstracts of the Fourth International Congress on Photosynthesis New York & London:: Academic Press.;
    [Google Scholar]
  14. HALLIWELL B. 1978 a; Biochemical mechanisms accounting for the toxic action of oxygen on living organisms: the key role of superoxide dismutase. Cell Biology International Reports 2:113–128
    [Google Scholar]
  15. HALLIWELL B. 1978 b; The chloroplast at work.A review of modern developments in our understanding of chloroplast metabolism. Progress in Biophysics and Molecular Biology 33:1–54
    [Google Scholar]
  16. HENRY L.E.A, GOGOTOV I.N., HALL D. 0. 1978; Superoxide dismutase and catalase in the protection of the proton-donating systems of nitrogen fixation in the blue-green algaAnabaena cylindrica . Biochemical Journal 174:373–377
    [Google Scholar]
  17. LITTLE C, O’BRIEN P. J. 1968; An intracellular GSH-peroxidase with a lipid peroxide substrate. Biochemical and Biophysical Research Communi-cations 31:145–150
    [Google Scholar]
  18. LORCH S. K, WOLK C. P. 1974; Application of gas-liquidchromatographyto study of the envelope lipids of heterocysts. Journal of Phycology 10:352–355
    [Google Scholar]
  19. LOWRY O.H, ROSEBROUGH N. J, FARR A. L, RANDALL R. J. 1951; Protein measurementwith the Folin phenol reagent. Journal of Biological Chemistry 193:193–275
    [Google Scholar]
  20. MAPSON L. W. 1958; Metabolismof ascorbic acid in plants: function. Annual Review of Plant Physiology 9:119–150
    [Google Scholar]
  21. MCCORD J. M, FRIDOVICH I. 1969; Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). Journal of Biological Chemistry 244:6049–6055
    [Google Scholar]
  22. MEEKS J. C, WOLK C. P, LOCKAU W, SCHILLING N, SHAFFR P. W, CHIEN W.S. 1978; Pathways of assimilation of [lSN]N2 and lSNHp+by cyanobacteria with and without heterocysts.. Journal of Bacteriology 134:125–130
    [Google Scholar]
  23. PATTERSON C. O.P., MYERS J. 1973; Photosynthetic production of hydrogen peroxide by Anacystis nidulans . Plant Physiology 51:104–109
    [Google Scholar]
  24. PATTERSON R. B., BURRIS R. H. 1978; Hydrogen metabolism in isolated heterocysts of Anabaena 7120 . Archives of Microbiology 51:104–109
    [Google Scholar]
  25. PATTERSON R. B., BURRIS R. H. 1978; Hydrogen metabolism in isolated heterocysts of Anabaena 7120 . Archives of Microbiology 116:125–132
    [Google Scholar]
  26. RIVERA-ORTIZ J. M., BURRIS R. H. 1975; nteractions among substrates and inhibitors of nitrogenase. Journal of Bacteriology 123:537–545
    [Google Scholar]
  27. ROE J. H. Chemical determination of ascorbic, dehydroascorbic and diketogulonic acids. in Glick. D. Methods of Biochemical Analysis, 1st edn, PP New York and London: Interscience; vol. 1:115–139 1954
    [Google Scholar]
  28. SCOTT E.M, BURRISDUNCAN I. W, EKSTRAND V. 1963; Purification and properties of glutathione reductase of human erythrocytes. Journal of Biological Chemistry 238:3928–3933
    [Google Scholar]
  29. SINGH P. K. 1973; Nitrogen fixation by the unicellular blue-green alga Aphanothece . Archiv für Mikrobiologie 92: 59–62
    [Google Scholar]
  30. STANIER R,. Y, COHEN-BAZRE G. 1977; Phototrophic prokaryotes: the cyanobacteria. Annual Review of Microbiology 31:225–274
    [Google Scholar]
  31. TEL-OR E, STEWART W.D.P. 1977; Photosynthetic components and activities of nitrogenfixing isolated heterocysts of Anabaena cylindrica . Proceedings of the Royal Society B198:61–86
    [Google Scholar]
  32. TIEBE F. 1969; Enzymic method for quantitative determinationof nanogram amounts of total and oxidised glutathione. Application to mammalian bloodand other tissues. Analytical Biochemistry 27:502–522
    [Google Scholar]
  33. TÖZüM D., UL-HAQUE M. I., CHAPLIN A. E., GALLON J. R. . 1977; The effectof fluoroacetate on acetylene reduction by Gloeocapsa . Biochemical Society Transaction 5:1482–1484
    [Google Scholar]
  34. WILCOCKSON J. 1977; The apparent influence of atmosphericoxygen concentration on nitrogenase activity and slime production in Klebsiella pneumoniae grown on a solid medium.. Journal of General Microbiology 101: 311–317
    [Google Scholar]
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