Summary: Polyacrylamide gel electrophoresis of bacterial lysates in a flat bed gives a linear relationship between In mol. wt of the proteins and the square root of their migration distances, thereby allowing standardization of different electrophoresis runs and precise comparison between homologous bands. The results obtained with , and strains were used in numerical analysis. and showed a greater internal similarity than , while two strains of the latter clustered with . The method described allows the comparison of mycobacteria with different generation times and provides a large number of good characters for numerical taxonomy.


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