@article{mbs:/content/journal/micro/10.1099/00221287-111-1-137, author = "Hopwood, David A. and Wright, Helen M.", title = "Factors Affecting Recombinant Frequency in Protoplast Fusions of Streptomyces coelicolor", journal= "Microbiology", year = "1979", volume = "111", number = "1", pages = "137-143", doi = "https://doi.org/10.1099/00221287-111-1-137", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-111-1-137", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "The optimum concentration of polyethylene glycol 1000 (PEG) for the production of recombinants through protoplast fusion in Streptomyces coelicolor was about 50% (w/v). The addition of 14% (v/v) dimethyl sulphoxide to the fusion mixture enhanced recombination frequencies, but only at sub-optimal PEG concentrations. After treatment of protoplasts with 50% PEG for 1 min, the frequency of recombinants in a multi-factor ‘cross’ sometimes exceeded 20% of the total progeny. The frequency of recombinants in the progeny could be significantly enhanced by ultraviolet irradiation of the parental protoplast suspensions immediately before fusion.", }