1887

Abstract

The optimum concentration of polyethylene glycol 1000 (PEG) for the production of recombinants through protoplast fusion in was about 50% (w/v). The addition of 14% (v/v) dimethyl sulphoxide to the fusion mixture enhanced recombination frequencies, but only at sub-optimal PEG concentrations. After treatment of protoplasts with 50% PEG for 1 min, the frequency of recombinants in a multi-factor ‘cross’ sometimes exceeded 20% of the total progeny. The frequency of recombinants in the progeny could be significantly enhanced by ultraviolet irradiation of the parental protoplast suspensions immediately before fusion.

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/content/journal/micro/10.1099/00221287-111-1-137
1979-03-01
2022-01-27
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