RT Journal Article SR Electronic(1) A1 Kopecká, Marie A1 Farkaš, VladimirYR 1979 T1 RNA Synthesis and the Formation of the Cell Wall. Effect of Lomofungin on Regenerating Protoplasts of Saccharomyces cerevisiae JF Microbiology, VO 110 IS 2 SP 453 OP 463 DO https://doi.org/10.1099/00221287-110-2-453 PB Microbiology Society, SN 1465-2080, AB SUMMARY: While lomofungin at 20 μg ml−1 effectively halted the synthesis of ribonucleic acids in protoplasts of Saccharomyces cerevisiae, the onset of biogenesis of new wall on the protoplast surface was not impaired. The synthesis of mannan-protein wall matrix continued for about 30 min after addition of lomofungin while the formation of 1,3-β-glucan networks was not influenced even after prolonged incubation. These results suggest that the life-span of limiting mRNA molecules coding for the proteins of the wall matrix is about 30 min while the turnover of 1,3-β-glucan synthases is relatively low., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-110-2-453