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Abstract
SUMMARY: Bacillus megaterium NCIB 7581, when growing exponentially in a simple chemically defined medium (without 2,6-diaminopimelic acid), contained only 4 mm-diaminopimelate in the free amino acid pool; this diaminopimelate was 84% (w/w) meso-isomer and 16% ll-isomer. Growing organisms could take up any of the three isomers of diaminopimelate from the medium, though the dd-isomer was taken up at only half the rate of the other two isomers (meso- and ll-). When lysine was also present in the medium, most (70%, w/w) of the diaminopimelate that was taken up entered peptidoglycan; only about 5% of the total uptake was into the free amino acid pool. When dd-diamino[14C]pimelate was supplied in the medium, 86% of the diamino[14C]pimelate incorporated into peptidoglycan was present as the meso-isomer and 14% was the dd-isomer.
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