@article{mbs:/content/journal/micro/10.1099/00221287-110-2-351, author = "Albertini, Alessandra M. and Baldi, M. Luisa and Ferrari, Eugenio and Isnenghi, Edoardo and Zambelli, M. Teresa and Galizzi, Alessandro", title = "Mutants of Bacillus subtilis Affected in Spore Outgrowth", journal= "Microbiology", year = "1979", volume = "110", number = "2", pages = "351-363", doi = "https://doi.org/10.1099/00221287-110-2-351", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-110-2-351", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: Six mutants of Bacillus subtilis 168 that are temperature-sensitive in spore outgrowth were isolated. The outgrowth process proceeds normally at 35 °C, but at the non-permissive temperature (47 °C) it is arrested at a specific stage characteristic for each mutant strain. The mutants are not altered in vegetative growth whether at 35 °C or at 47 °C. They were characterized for their ability to synthesize RNA, proteins and DNA during outgrowth. A mutant defective in spore germination was also isolated; less than 5% of its spores can germinate at any of the temperatures tested. The mutations were mapped by means of transduction and transformation. The isolation of a number of outgrowth mutants which map at different loci and which affect outgrowth at different times is discussed in relation to the regulation of this process.", }