1887

Abstract

SUMMARY: Trehalose, a storage sugar of baker's yeast, is known not to be metabolized when added to a cell suspension in water or a growth medium and to support growth only after a lag of about 10 h. However, it was transported into cells by at least two transport systems, the uptake being active, with a pH optimum at 5·5. There was no stoicheiometry with the shift of protons into cells observed at high trehalose concentrations. Trehalose remained intact in cells and was not appreciably lost to a trehalose-free medium. The uptake systems were present directly after growth on glucose, then decayed with a half-life of about 25 min but could be reactivated by aerobic incubation with trehalose, maltose, α-methyl-D-glucoside, glucose or ethanol. The uptake systems thus induced were different as revealed by competition experiments. At least one of the systems for trehalose uptake showed cooperative kinetics. Comparative analysis with other disaccharides indicated the existence in , after induction with trehalose, of at least four systems for the uptake of α-methyl-D-glucoside, four systems for maltose, together with the two for trehalose, variously shared by the sugars, the total of α-glucoside-transporting systems being five.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-110-2-323
1979-02-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/110/2/mic-110-2-323.html?itemId=/content/journal/micro/10.1099/00221287-110-2-323&mimeType=html&fmt=ahah

References

  1. Alonso A., Kotyk A. 1978; Apparent half-lives of sugar-transport proteins in baker’s yeast. Folia microbiologica 23:118–125
    [Google Scholar]
  2. Candy D. J., Kilby B. A. 1959; Site and mode of trehalose biosynthesis in the locusts. Nature, London 183:1594–1595
    [Google Scholar]
  3. Görts C. P. M. 1969; Effect of glucose on the activity and the kinetics of the maltose uptake system and of α-glucosidase in Saccharomyces cerevisiae. Antonie van Leeuwenhoek 35:233–234
    [Google Scholar]
  4. Harris G., Thompson C. C. 1961; The uptake of nutrients by yeasts. III. The maltose permease of a brewing yeast. Biochimica et biophysica acta 52:176–183
    [Google Scholar]
  5. Holzer H. 1977; Function and regulation of yeast intracellular proteinases.. In EUCHEM Conference on Metabolic Reactions in the Yeast Cell, Helsinki, pp. 21–22
    [Google Scholar]
  6. Horák J., Kotyk A., Říhová L. 1978; Stimulation of amino acid transport in Saccharomyces cerevisiae by metabolic inhibitors. Folia micro-biologica 23:286–291
    [Google Scholar]
  7. Janda S., Kotyk A. 1972; Some features of carbohydrate metabolism in Rhodotorula glutinis. Folia microbiologica 17:461–470
    [Google Scholar]
  8. Kotyk A. 1967; Properties of the sugar carrier in baker’s yeast. III. Specificity of transport. Folia microbiologica 12:121–131
    [Google Scholar]
  9. Kotyk A. 1979; Different energy sources for membrane transport in yeasts. Pure and Applied Chemistry (in the Press)
    [Google Scholar]
  10. Kotyk A., Michaljaničová D. 1974; Nature of uptake of d-galactose, d-glucose and α-methyl-d-glucoside by Saccharomyces cerevisiae. Biochimica et biophysica acta 332:104–113
    [Google Scholar]
  11. Kotyk A., Michaljaničová D., Veres K., Soukupova V. 1975; Transport of 4-deoxy- and 6-deoxy-d-glucose in baker’s yeast. Folia micro-biologica 20:496–503
    [Google Scholar]
  12. Laidler K. J., Bunting P. S. 1973 The Chemical Kinetics of Enzyme Action, 2nd edn. Oxford: Clarendon Press;
    [Google Scholar]
  13. Lodder J. 1970 The Yeasts Amsterdam: North Holland;
    [Google Scholar]
  14. Okada H., Halvorson H. O. 1964; Uptake of α-thioethyl-d-pyranoside by Saccharomyces cerevisiae. II. General characteristics of an active transport system. Biochimica et biophysica acta 82:547–555
    [Google Scholar]
  15. Panek A. 1963; Function of trehalose in baker’s yeast (Saccharomyces cerevisiae). Archives of Biochemistry and Biophysics 100:422–425
    [Google Scholar]
  16. Panek A., Mattoon J. R. 1977; Regulation of energy metabolism in Saccharomyces cerevisiae. Archives of Biochemistry and Biophysics 183:306–316
    [Google Scholar]
  17. Seaston A., Inkson C., Eddy A. A. 1973; The absorption of protons with specific amino acids and carbohydrates by yeast. Biochemical Journal 134:1031–1043
    [Google Scholar]
  18. Serrano R. 1977; Energy requirements for maltose transport in yeast. European Journal of Biochemistry 80:97–102
    [Google Scholar]
  19. Siro M. R., Hautera P., Lövgren T. 1977; Influence of glucose on the maltose uptake of Saccharomyces cerevisiae.. In EUCHEM Conference on Metabolic Reactions in the Yeast Cell, Helsinki, pp. 61–62
    [Google Scholar]
  20. Sussman A. S. 1961; The role of trehalose in the activation of dormant ascospores of Neurospora. Quarterly Review of Biology 36:109–116
    [Google Scholar]
  21. Zimmermann F. K., Khan N. A., Eaton N. R. 1973; Identification of new genes involved in disaccharide fermentation in yeast. Molecular and General Genetics 123:29–41
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-110-2-323
Loading
/content/journal/micro/10.1099/00221287-110-2-323
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error