1887

Abstract

SUMMARY: R plasmid R772 was isolated from a strain of and is a self-transmissible P-1 incompatibility group plasmid having a molecular weight of about 27 × 10. It renders bacterial hosts resistant to kanamycin. Phage PR772 was isolated as a phage dependent on the presence of R772 in bacterial hosts. It is hexagonal-shaped with a diameter of 53 nm, has a thick inner membrane and no tail. Vaguely defined appendages are sometimes apparent at some vertices and the phage possesses double-stranded DNA. The DNA has a guanine plus cytosine molar content of 48%. The phage is sensitive to chloroform and has a buoyant density of 1·26 g cm. These observations suggested that the inner membrane of the phage could contain lipid. Phage PR772 differs in morphology from the double-stranded DNA plasmid-specific phages PR4 and PRR1 which adsorb to tips and sides, respectively, of sex pili coded for by P-1 incompatibility group plasmids. Phage PR772 formed clear plaques which varied in diameter. Serologically, phages PR772 and PR4 are possibly related though very distantly, but the two phages have identical host ranges. Phage PR772 adsorbed by one of its apices to tips of sex pili coded for by plasmid R772 in . It also formed plaques on and Providence strains harbouring this plasmid as well as strains of carrying plasmids of incompatibility groups N or W. The phage produced areas of partial clearing on lawns of PM5006 harbouring plasmid R772, the P-1 incompatibility group plasmid RP4, the W group plasmid RSa or the N group plasmid N3, and on lawns of Providence strain P29 carrying plasmid RP4.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-110-2-263
1979-02-01
2021-05-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/110/2/mic-110-2-263.html?itemId=/content/journal/micro/10.1099/00221287-110-2-263&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1956; Methods of study of bacterial viruses. Methods in Medical Research 2:1–73
    [Google Scholar]
  2. Baumberg S., Dennison S. 1975; Variation in expression of sex factor genes in the Proteus-Providencia group relative to Escherichia coli. Journal of Bacteriology 123:278–286
    [Google Scholar]
  3. Bradley D. E. 1972a; Shortening of Pseudomonas aeruginosa pili after RNA-phage adsorption. Journal of General Microbiology 72:303–319
    [Google Scholar]
  4. Bradley D. E. 1972b; Evidence for the retraction of Pseudomonas aeruginosa RNA phage pili. Biochemical and Biophysical Research Communications 47:142–149
    [Google Scholar]
  5. Bradley D. E. 1973; The adsorption of the Pseudomonas aeruginosa filamentous bacteriophage Pf to its host. Canadian Journal of Microbiology 19:623–631
    [Google Scholar]
  6. Bradley D. E. 1974; Adsorption of bacteriophages specific for Pseudomonas aeruginosa R factors RP1 and R1822. Biochemical and Biophysical Research Communications 57:893–900
    [Google Scholar]
  7. Bradley D. E. 1975; The occurrence of pili associated with a plasmid of the W compatibility group. Biochemical and Biophysical Research Communications 64:918–925
    [Google Scholar]
  8. Bradley D. E. 1976; Adsorption of the R-specific bacteriophage PR4 to pili determined by a drug resistance plasmid of the W compatibility group. Journal of General Microbiology 95:181–185
    [Google Scholar]
  9. Bradley D. E. 1977; Characterization of pili determined by drug resistance plasmids R711b and R778b. Journal of General Microbiology 102:349–363
    [Google Scholar]
  10. Bradley D. E., Cohen D. R. 1977; Adsorption of lipid-containing bacteriophages PR4 and PRD1 to pili determined by a P-l incompatibility group plasmid. Journal of General Microbiology 98:619–623
    [Google Scholar]
  11. Bradley D. E., Rutherford E. L. 1975; Basic characterization of lipid-containing bacteriophage specific for plasmids of the P, N, and W compatibility groups. Canadian Journal of Microbiology 21:152–163
    [Google Scholar]
  12. Brodt P., Leggett F., Iyer R. 1974; Absence of a pilus receptor for filamentous phage IKe. Nature, London 249:856–858
    [Google Scholar]
  13. Bruner R., Vinograd J. 1965; The evaluation of standard sedimentation coefficients of sodium RNA and sodium DNA from sedimentation velocity data in concentrated NaCl and CsCl solutions. Biochimica et biophysica acta 108:18–29
    [Google Scholar]
  14. Bryan L. E., Semaka S. D., Van den Elzen H. M., Kinnear J. E., Whitehouse R. L. S. 1973; Characteristics of R931 and other Pseudomonas aeruginosa R factors. Antimicrobial Agents and Chemotherapy 3:625–637
    [Google Scholar]
  15. Caro L. G., Schnos M. 1966; The attachment of the male-specific bacteriophage F1 to sensitive strains of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 56:126–132
    [Google Scholar]
  16. Clowes R. C., Hayes W. 1968 Experiments in Microbial Genetics Oxford and Edinburgh: Blackwell Scientific Publications;
    [Google Scholar]
  17. Coetzee J. N. 1966; Transduction in Proteus morganii. Nature, London 210:220
    [Google Scholar]
  18. Coetzee J. N. 1974; High frequency transduction of kanamycin resistance in Proteus mirabilis. Journal of General Microbiology 84:285–296
    [Google Scholar]
  19. Coetzee J. N. 1978; Mobilization of the Proteus mirabilis chromosome by R plasmid R772. Journal of General Microbiology 108:103–109
    [Google Scholar]
  20. Coetzee J. N., Smit J. A. 1970; Properties of Proteus mirabilis phage 13 vir. Journal of General Virology 9:247–249
    [Google Scholar]
  21. Coetzee J. N., Smit J. A., Prozesky O. W. 1966; Properties of Providence and Proteus morganii transducing phages. Journal of General Microbiology 44:167–176
    [Google Scholar]
  22. Coetzee J. N., Datta N., Hedges R. W. 1972; R factors from Proteus rettgeri. Journal of General Microbiology 72:543–552
    [Google Scholar]
  23. Coetzee J. N., Krizsanovich-Williams K., Williams J. A. 1977; Cotransduction of mor- ganocinogenic plasmid 174 and R factor R772. Journal of General Microbiology 100:299–308
    [Google Scholar]
  24. Datta N., Hedges R. W. 1972; Host ranges of R factors. Journal of General Microbiology 70:453–460
    [Google Scholar]
  25. Datta N., Hedges R. W., Shaw E. J., Sykes R. P., Richmond M. H. 1971; Properties of an R factor from Pseudomonas aeruginosa. Journal of Bacteriology 108:1244–1249
    [Google Scholar]
  26. Dennison S., Baumberg S. 1975a; Unusual characteristics of the receptor for the N sex factor-specific filamentous phage IKe. Genetical Research 25:275–284
    [Google Scholar]
  27. Dennison S., Baumberg S. 1975b; Conjugational behaviour of N plasmids in Escherichia coli K12. Molecular and General Genetics 138:323–331
    [Google Scholar]
  28. Falkow S., Guerry P., Hedges R. W., Datta N. 1974; Polynucleotide sequence relationships among plasmids of the I compatibility complex. Journal of General Microbiology 85:65–76
    [Google Scholar]
  29. Franklin R. M. 1974; Structure and synthesis of bacteriophage PM2, with particular emphasis on the viral lipid bilayer. Current Topics in Microbiology and Immunology 68:107–159
    [Google Scholar]
  30. Grindley N. D. F., Humphreys G. O., Anderson E. S. 1973; Molecular studies of R factor compatibility groups. Journal of Bacteriology 115:387–398
    [Google Scholar]
  31. Haas D., Holloway B. W. 1976; R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Molecular and General Genetics 144:243–251
    [Google Scholar]
  32. Hedges R. W. 1972; Phenotypic characterization of fi R factors determining the restriction and modification of hsp II specificity. Molecular and General Genetics 115:225–233
    [Google Scholar]
  33. Hedges R. W. 1975; R factors from Proteus mirabilis and P. vulgaris. Journal of General Microbiology 87:301–311
    [Google Scholar]
  34. Hedges R. W., Datta N. 1971; fi R factors giving chloramphenicol resistance. Nature, London 234:220–221
    [Google Scholar]
  35. Hedges R. W., Jacob A. E. 1974; Transposition of ampicillin resistance from RP4 to other replicons. Molecular and General Genetics 132:31–40
    [Google Scholar]
  36. Hedges R. W., Jacob A. E. 1975; Mobilization of plasmid-borne drug resistance determinants for transfer from Pseudomonas aeruginosa to Escherichia eoli. Molecular and General Genetics 140:69–79
    [Google Scholar]
  37. Jacoby G. A. 1974; Properties of R plasmids determining gentamicin resistance by acetylation in Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy 6:239–252
    [Google Scholar]
  38. Jobanputra R. A., Datta N. 1974; Trimethoprim R factors in enterobacteria from clinical specimens. Journal of Medical Microbiology 7:169–177
    [Google Scholar]
  39. Khatoon H., Iyer R. V., Iyer V. N. 1972; A new filamentous bacteriophage with sex-factor specificity. Virology 48:145–155
    [Google Scholar]
  40. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods in Enzymology 12:195–206
    [Google Scholar]
  41. Marmur J., Doty P. 1962; Determination of base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  42. Meynell E., Cooke M. 1969; Repressor- minus and operator-constitutive de-repressed mutants of F-like R factors: their effect on chromosomal transfer by HfrC. Genetical Research 14:309–313
    [Google Scholar]
  43. Meynell E., Datta N. 1967; Mutant drug resistance factors of high transmissibility. Nature, London 214:885–887
    [Google Scholar]
  44. Meynell G. G. 1972 Bacterial Plasmids London: MacMillan;
    [Google Scholar]
  45. Meynell G. G., Lawn A. M. 1968; Filamentous phages specific for the I sex factor. Nature, London 217:1184–1186
    [Google Scholar]
  46. Morgan E. A., Kaplan S. 1977; Expression and stability of Escherichia eoli F-prime factors in Proteus mirabilis. Molecular and General Genetics 151:41–47
    [Google Scholar]
  47. Olsen R. H., Thomas D. D. 1973; Characteristics and purification of PRRl, an RNA phage specific for the broad host range Pseudomonas R1882 drug resistance plasmid. Journal of Virology 12:1560–1567
    [Google Scholar]
  48. Olsen R., H„ Siak J.-S., Gray R. H. 1974; Characteristics of PRD1, a plasmid-dependent broad host range DNA bacteriophage. Journal of Virology 14:689–699
    [Google Scholar]
  49. Sands J. A., Cupp J., Keith A., Snipes W. 1974; Temperature sensitivity of the assembly process of the enveloped bacteriophage ϕ6. Biochimica et biophysica acta 373:277–285
    [Google Scholar]
  50. Shahrabadi M. S., Bryan L. E., Van den Elzen H. M. 1975; Further properties of P-2 R-factors of Pseudomonas aeruginosa and their relationship to other plasmid groups. Canadian Journal of Microbiology 21:592–605
    [Google Scholar]
  51. Smith D. I., Gomez Lus R., Rubio Calvo M. C., Datta N., Jacob A. E., Hedges R. W. 1975; Third type of plasmid conferring gentamicin resistance in Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy 8:227–230
    [Google Scholar]
  52. Stanisich V. A. 1974; The properties and host range of male-specific bacteriophages of Pseudomonas aeruginosa. Journal of General Microbiology 84:332–342
    [Google Scholar]
  53. Vidaver A. K., Koski R. K., Van Etten J. L. 1973; Bacteriophage ϕ6: a lipid-containing virus of Pseudomonas phaseolicola. Journal of Virology 11:799–805
    [Google Scholar]
  54. Williams J. A. 1977; Mobilization of morganocin 174 plasmid and kinetics of morganocin production in Proteus and Escherichia eoli hosts. Antimicrobial Agents and Chemotherapy 11:514–520
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-110-2-263
Loading
/content/journal/micro/10.1099/00221287-110-2-263
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error