1887

Abstract

SUMMARY: Nitrogen starvation and heterocyst development were induced in the cyanobacterium 7120 by growth in nitrogen-free medium or by treatment with the amino acid analogue methionine sulphoximine. During the first 6 h of nitrogen deprivation, amino acid levels and rates of protein synthesis, as measured by the incorporation of [H]leucine, decreased to 50 to 70% of those in ammonia-grown organisms; after this time there was no difference between the rates of protein synthesis in ammonia-grown and nitrogen-starved cultures. The period 4 to 12 h after the onset of starvation was marked by the release of [H]leucine from previously labelled proteins at a rate 6 to 7·5 times that of ammonia-grown organisms. These results are consistent with the idea that nitrogen starvation in cyanobacteria causes a reduction in protein synthesis and leads to the rapid degradation of storage proteins. In rapidly growing 7120, the doubling time for total cell protein was estimated to be 14·9 ± 1·0 h and the half-life was 139 ± 88 h.

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1979-02-01
2024-11-11
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References

  1. Allen M. B., Arnon D. I. 1955; Studies on nitrogen-fixing blue-green algae. I. Growth and nitrogen fixation by Anabaena cylindrica Lemm. Plant Physiology 30:366–372
    [Google Scholar]
  2. Bradley S., Carr N. G. 1976; Heterocyst and nitrogenase development in Anabaena cyfindrica. Journal of General Microbiology 96:175–184
    [Google Scholar]
  3. Doi R. H. 1972; Role of proteases in sporulation. Current Topics in Cellular Regulation 6:1–18
    [Google Scholar]
  4. Fleming H., Haselkorn R. 1974; The program of protein synthesis during heterocyst differentiation in nitrogen-fixing blue-green algae. Cell 3:159–170
    [Google Scholar]
  5. Foulds I. J., Carr N. G. 1977; A proteolytic enzyme degrading phycocyanin in the cyanobacterium Anabaena cylindrica. FEMS Microbiology Letters 2:117–119
    [Google Scholar]
  6. Goldberg A. L., St John A. C. 1976; Intracellular protein degradation in mammalian and bacterial cells: part 2. Annual Review of Biochemistry 45:747–803
    [Google Scholar]
  7. Halvorson H. 1958; Intracellular protein and nucleic acid turnover in resting yeast cells. Biochimica et biophysica acta 27:255–266
    [Google Scholar]
  8. Huffaker R. C., Peterson L. W. 1974; Protein turnover in plants and possible means of its regulation. Annual Review of Plant Physiology 25:363–392
    [Google Scholar]
  9. Lau R. H., , Mackenzie M. M., Doolittle W. F. 1977; Phycocyanin synthesis and degradation in the blue-green bacterium Anacystis nidulans. Journal of Bacteriology 132:771–778
    [Google Scholar]
  10. Liao T., , Robinson G. W., Salnikow J. 1973; Use of narrow-bore columns in amino acid analysis. Analytical Chemistry 45:2286–2288
    [Google Scholar]
  11. Lowry O. H., , Rosebrough N. J., , Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  12. Mandelstam J. 1958; Turnover of protein in growing and non-growing populations of Escherichia coli. Biochemical Journal 69:110–119
    [Google Scholar]
  13. Mandelstam J., Waites W. M. 1968; Sporulation in Bacillus subtilis. The role of exoprotease. Biochemical Journal 109:793–801
    [Google Scholar]
  14. Moore S., Stein W. H. 1954; Procedures for the chromatographic determination of amino acids on four per cent cross-linked sulfonated polystyrene resins. Journal of Biological Chemistry 211:893–906
    [Google Scholar]
  15. Newman E. B., , Adley T., , Fraser J., , Potter R., Kapoor V. 1976; The conversion of leucine to α-ketoisocaproic acid and its metabolic consequences for Escherichia coli K12. Canadian Journal of Microbiology 22:922–928
    [Google Scholar]
  16. Ownby J. 1977; Effects of amino acids on methionine sulfoximine-induced heterocyst formation in Anabaena. Planta 136:277–279
    [Google Scholar]
  17. Scherer C. G., Boylen C. W. 1977; Macromolecular synthesis and degradation in Arthrobacter during periods of nutrient starvation. Journal of Bacteriology 132:584–589
    [Google Scholar]
  18. Stewart W. D. P., Rowell P. 1975; Effects of l-methionine-dl-sulfoximine on the assimilation of newly fixed NH3, acetylene reduction, and heterocyst production in Anabaena cylindrica. Biochemical and Biophysical Research Communications 65:846–856
    [Google Scholar]
  19. Talpasayi E. R. S., Kale K. S. 1967; Induction of heterocysts in the blue-green alga Anabaena ambigua. Current Science 36:218–219
    [Google Scholar]
  20. Thomas J., , Wolk C. P., , Shaffer P. W., , Austin S. M., Galonsky A. 1975; The initial organic products of fixation of 15N-labelled nitrogen gas by the blue-green alga Anabaena cylindrica. Biochemical and Biophysical Research Communications 67:501–507
    [Google Scholar]
  21. Trewavas A. 1972; Determination of the rates of protein synthesis and degradation in Lemna minor. Plant Physiology 49:40–46
    [Google Scholar]
  22. Willetts N. S. 1967; Intracellular protein breakdown in growing cells of Escherichia coli. Biochemical Journal 103:462–466
    [Google Scholar]
  23. Wood N. B., Haselkorn R. 1976; Protein degradation during heterocyst development in Anabaena.. In Proceedings of the Second International Symposium on Photosynthetic Prokaryotes , pp. 125–127 Edited by Codd G. A., Stewart W. D. P. Dundee: University of Dundee Press;
    [Google Scholar]
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