1887

Abstract

Summary: Octopine-utilizing agrobacteria could be distinguished from non-utilizers on a solid medium containing octopine as the only added nitrogen source if a pH indicator such as bromothymolblue were present. On green bromothymolblue plates, octopine-utilizing strains formed orange-yellow colonies, while those of non-utilizers were translucent. Strains could be tested for utilization of other nitrogen sources such as nopaline by substituting these for octopine in the same medium.

Using bromothymolblue plates, it was found that TI plasmids of octopine-utilizing strains of were transferable in the presence of octopine, octopinic acid or lysopine, but not in the presence of the analogues nor-octopine, homo-octopine or desmethylhomo-octopine. Transfer in the presence of “inducer” was not detected when bacteria were mated at 37 °C instead of 29 °C, or when either methionine, cysteine or cystine was present in the mating medium. Mutant plasmids were obtained that were conjugative in the absence of an inducer; these were insensitive to methionine and cysteine but, like the wild-type plasmid, were thermosensitive for transfer.

The TI plasmid of an octopine-utilizing strain was introduced into a strain that carried a deleted nopaline TI plasmid. These plasmids were found to be compatible. The properties of this transconjugant showed that the octopine oxidase does not accept nopaline as a substrate. Tumours induced on by this bacterium were rough and contained octopine. Exclusion of phage S18 was found to be a marker specific for the TI(Kerr14) plasmid.

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1979-01-01
2021-07-26
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References

  1. Bernaerts M., De Ley J. 1963; A biochemical test for crown gall bacteria.. Nature, London 197:406–407
    [Google Scholar]
  2. Bomhoff G. H. 1974; Studies on crown gall–a plant tumour. Investigations on protein composition and on the use of guanidine compounds as a marker for transformed cells. Thesis University of Leiden, The Netherlands;
    [Google Scholar]
  3. Bomhoff G. H., Klapwijk P. M., Kester H. C. M., Schilperoort R. A., Hernalsteens J. P., Schell J. 1976; Octopine and nopaline: synthesis and breakdown genetically controlled by a plasmid of Agrobacterium tumefaciens. Molecular and General Genetics 145:177–181
    [Google Scholar]
  4. Braun A. C. 1947; Thermal studies on the factors responsible for tumor initiation in crown gall.. American Journal of Botany 34:234–240
    [Google Scholar]
  5. Braun A. C. 1950; Thermal inactivation studies on the tumor-inducing principle in crown gall.. Phytopathology 40:3
    [Google Scholar]
  6. Chilton M.-D., Farrand S. K., Levin R., Nester E. W. 1976; RP4 promotion of transfer of a large Agrobacterium plasmid which confers virulence.. Genetics 83:609–618
    [Google Scholar]
  7. Chilton M.-D., Drummond M. H., Merlo D. J., Sciaky D., Montoya A. L., Gordon M. P., Nester E. W. 1977; Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis.. Cell 11:263–271
    [Google Scholar]
  8. Datta N., Hedges R. W. 1972; Host ranges of R factors.. Journal of General Microbiology 70:453–460
    [Google Scholar]
  9. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. 1971; Properties of an R factor from Pseudomonas aeruginosa. Journal of Bacteriology 108:1244–1249
    [Google Scholar]
  10. Deep I. W., Hussin H. 1962; Effect of postinoculation temperature on crown gall development on cherry.. Phytopathology 52:360
    [Google Scholar]
  11. Drummond M. H., Gordon M. P., Nester E. W., Chilton M.-D. 1977; Foreign DNA of bacterial plasmid origin is transcribed in crown gall tumours.. Nature, London 269:535–536
    [Google Scholar]
  12. Genetello C., van Larebeke N., Holsters M., De Picker A., Van Montagu M., Schell J. 1977; The Ti plasmids of Agrobacterium as sex factors.. Nature, London 265:561–563
    [Google Scholar]
  13. Gordon M. P., Farrand S. K., Sciaky D., Montoya A., Chilton M.-D., Merlo D., Nester E. W. 1978; In Molecular Biology of Plants, Symposium, University of Minnesota, 1976. Edited by Rubenstein I. New York: Academic Press (in the Press);
    [Google Scholar]
  14. Haas D., Holloway B. W. 1976; R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Molecular and General Genetics 144:243–251
    [Google Scholar]
  15. Hedges R. W., Jacob A. E. 1974; Transposition of ampicillin resistance from RP4 to other replicons.. Molecular and General Genetics 132:31–40
    [Google Scholar]
  16. Hernalsteens J. P., Engler G., Van Larebeke N., van Montagu M., Schell J. 1975; Studies on large DNA plasmids of Agrobacterium tumefaciens. Archives internationales de physiologie et de biochimie 83:368–369
    [Google Scholar]
  17. Hooykaas P. J. J., Klapwijk P. M., Nuti M. P., Schilperoort R. A., Rörsch A. 1977; Transfer of the Agrobacterium tumefaciens TI plasmid to avirulent agrobacteria and to Rhizobium ex planta. Journal of General Microbiology 98:477–84
    [Google Scholar]
  18. Kemp J. D. 1976; Octopine as a marker for the induction of tumorous growth by Agrobacterium tumefaciens strain b6.. Biochemical and Biophysical Research Communications 69:816–822
    [Google Scholar]
  19. Kerr A. 1971; Acquisition of virulence by non-pathogenic isolates of Agrobacterium radiobacter. Physiological Plant Pathology 1:241–246
    [Google Scholar]
  20. Kerr A., Manigault P., Tempé J. 1977; Transfer of virulence in vivo and in vitro in Agrobacterium. Nature, London 265:560–561
    [Google Scholar]
  21. Klapwijk P. M., Hooykaas P. J. J., Kester H. C. M., Schilperoort R. A., Rörsch A. 1976; Isolation and characterization of Agrobacterium tumefaciens mutants affected in the utilization of octopine, octopinic acid and Iysopine.. Journal of General Microbiology 96:155–163
    [Google Scholar]
  22. Klapwijk P. M., Oudshoorn M., Schilperoort R. A. 1977; Inducible permease involved in the uptake of octopine, Iysopine and octopinic acid by Agrobacterium tumefaciens strains carrying virulence-associated plasmids.. Journal of General Microbiology 102:1–11
    [Google Scholar]
  23. Larebeke N., Van, Genetello C., Schell J., Schilperoort R. A., Hermans A. K., Hernalsteens J. P., van Montagu M. 1975; Acquisition of tumor inducing ability by non-oncogenic Agrobacteria as a result of plasmid transfer.. Nature, London 255:742–743
    [Google Scholar]
  24. Larebeke N., Van, Genetello C., Hernalsteens J. P., De Picker A., Zaenen I., Messens E., Van Montagu M., Schell J. 1977; Transfer of Ti-plasmids between Agrobacterium strains by mobilisation with the conjugative plasmid RP4.. Molecular and General Genetics 152:119–124
    [Google Scholar]
  25. Lippincott B. B., Lippincott J. A. 1969; Bacterial attachment to a specific wound site as an essential stage in tumor initiation by Agrobacterium tumefaciens. Journal of Bacteriology 97:620–628
    [Google Scholar]
  26. Lippincott J. A., Beiderbeck R., Lippincott B. B. 1973; Utilization of octopine and nopaline by Agrobacterium. Journal of Bacteriology 116:378–383
    [Google Scholar]
  27. Lippincott B. B., Whatley M. H., Lippincott J. A. 1977; Tumour induction by Agrobacterium involves attachment of the bacterium to a site on the host plant cell wall.. Plant Physiology 59:388–390
    [Google Scholar]
  28. Montoya A. L., Chilton M.-D., Gordon M. P., Sciaky D., Nester E. W. 1977; Octopine and nopaline metabolism in Agrobacterium tumefaciens and crown gall tumor cells: role of plasmid genes.. Journal of Bacteriology 129:101–107
    [Google Scholar]
  29. Otten L. A. B. M., Vreugdenhil D., Schilperoort R. A. 1977; Properties of d(+)-lysopine dehydrogenase from crown gall tumour tissue.. Biochimica et biophysica acta 485:268–277
    [Google Scholar]
  30. Petit A., Tempé J. 1975; Étude du métabolisme des guanidines des tissus de crown-gall par la souche T37 d’Agrobacterium tumefaciens. Comptes rendus hebdomadaire des séances de l’Académie des sciences 281:467–469
    [Google Scholar]
  31. Petit A., Delhaye S., Tempé J., Morel G. 1970; Recherches sur les guanidines des tissus de crown gall. Mise en évidence d’une relation bio-chimique spécifique entre les souches d’Agrobacterium tumefaciens et les tumeurs qu’elles induisent.. Physiologie Végétale 8:205–213
    [Google Scholar]
  32. Riker A. J. 1926; Studies on the influence of some environmental factors on the development of crown gall.. Journal of Agricultural Research 32:83–96
    [Google Scholar]
  33. Roizès G., Patillon M., Kovoor A. 1977; A restriction endonuclease from Agrobacterium tumefaciens. FEBS Letters 82:69–70
    [Google Scholar]
  34. Schilperoort R. A. 1969; Investigations on plant tumors. Crown gall. On the biochemistry of tumor induction by Agrobacterium tumefaciens. Thesis University of Leiden, The Netherlands;
    [Google Scholar]
  35. Schilperoort R. A., Bomhoff G. H. 1975; Crown gall: a model for tumor research and genetic engineering. In Genetic Manipulations with Plant Material pp. 141–162 Edited by Ledoux L. New York: Plenum Press;
    [Google Scholar]
  36. Tempé J., Petit A., Hosters M., van Montagu M., Schell J. 1977; Thermosensitive step associated with transfer of Ti-plasmid during conjugation: possible relation to transformation in crown gall.. Proceedings of the National Academy of Sciences of the United States of America 74:2848–2849
    [Google Scholar]
  37. Watson B., Currier T. C., Gordon M. P., Chilton M.-D., Nester E. W. 1975; Plasmid required for virulence of Agrobacterium tumefaciens. Journal of Bacteriology 123:255–264
    [Google Scholar]
  38. Whatley M. H., Bodwin J. S., Lippincott B. B., Lippincott J. A. 1976; Role for Agrobacterium cell envelope lipopolysaccharide in infection site attachment.. Infection and Immunity 13:1080–1083
    [Google Scholar]
  39. Zaenen I., van Larebeke N., Teuchy H., Van Montagu M., Schell J. 1974; Supercoiled circular DNA in crown gall inducing Agrobacterium strains.. Journal of Molecular Biology 86:109–127
    [Google Scholar]
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