1887

Abstract

Summary: A phytotoxic extract of (cherry isolate c28) was shown to contain phytotoxic protein associated with bacteriophage-specific lipopolysaccharide. This and similar extracts from two other pathogenic pseudomonads produced a silvery effect when infiltrated at 100 μg ml (14·4 mu;g protein ml) into leaves of some plant species. The effect was similar to that caused by infection in cherry. In contrast to the fungal toxin, the bacterial extracts were not translocated in treated plants. Wax-embedded sections of cherry leaves silvered either by the fungus or by bacterial extracts showed optically active cells when stained with toluidine blue. Different plant species differentiated between extracts of different pseudomonads by their reaction to treatment. The hypersensitive response of tobacco to heterologous pathogenic bacteria was suppressed by prior treatment with extracts of cherry-type and saprophytic bacteria. Similar phytotoxic material isolated from culture filtrates of the cherry isolate produced the silvery effect in bean leaves when infiltrated at 10 μg ml (5 μg protein ml). The silvering agents (molecular weight in excess of 25 × 10 by gel filtration) were dissociated into smaller units by electrophoresis in sodium dodecyl sulphate-polyacrylamide gel. The activities described were destroyed by protein-inactivating treatments.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-110-1-77
1979-01-01
2021-05-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/110/1/mic-110-1-77.html?itemId=/content/journal/micro/10.1099/00221287-110-1-77&mimeType=html&fmt=ahah

References

  1. Bauchop J., Elsden S. R. 1960; The growth of micro-organisms in relation to their energy supply.. Journal of General Microbiology 23:457–469
    [Google Scholar]
  2. Brooks F. T., Brenchley G. G. 1931; Further injection experiments in relation to Stereum purpureum. New Phytologist 30:128–133
    [Google Scholar]
  3. Crosse J. E. 1959; Bacterial canker of stone fruits. IV. Investigation of a method for measuring the inoculum potential of cherry trees.. Annals of Applied Biology 47:306–317
    [Google Scholar]
  4. Erikson D. 1945; Certain aspects of resistance of plum trees to bacterial canker. I.. Annals of Applied Biology 32:44–52
    [Google Scholar]
  5. Garrett C. M. E., Crosse J. E., Sletten A. 1974; Relations between phage sensitivity and virulence in Pseudomonas morsprunorum. Journal of General Microbiology 80:475–483
    [Google Scholar]
  6. Hignett R. C. 1975; Vacuum infiltration of plant leaves in situ. Laboratory Practice 24:586
    [Google Scholar]
  7. Hignett R. C., Quirk A. V. 1975; Phytotoxins of plant pathogenic pseudomonads. In 10th International Congress F.E.B.S., Paris abstr. no. 1425 Paris: Société de Chimie Biologique;
    [Google Scholar]
  8. Klement Z. 1963; Rapid detection of pathogenicity of phytopathogenic pseudomonads.. Nature, London 199:299–300
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Quirk A. V., Sletten A., Hignett R. C. 1976; Properties of phage-receptor lipopolysaccharide from Pseudomonas morsprunorum. Journal of General Microbiology 96:375–381
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-110-1-77
Loading
/content/journal/micro/10.1099/00221287-110-1-77
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error