Summary: As the first stage in investigating the genetic basis of natural variation in Escherichia coli, the gene(s) conferring the ability to use sucrose as a carbon and energy source (given the symbol sac+) was transferred from a wild strain to k12, which does not use sucrose. The sac+ region was transferred by two different methods. On both occasions it took a chromosomal location at minute 50·5 on the linkage map, between aroC and supN, in the region of the dsd genes, which confer the ability to use d-serine as a carbon and energy source. When the sac+ region was present in the k12 chromosome the bacteria were unable to use d-serine as a carbon and energy source. In F'sac+/dsd+ diploids, the dsd+ genes were similarly not expressed. Strain k12(sac+) bacteria were sensitive to inhibition by d-serine; they mutated to d-serine resistance with much greater frequency than did a dsd mutant of k12. Such bacteria also mutated frequently to use raffinose. Strain k12(sac+) bacteria did not utilize sucrose when they carried a mutation affecting the phosphotransferase system.
AdelbergE. A.,
MandelM.,
Chein Ching ChenG.1965; Optimal conditions for mutagenesis by N-methyl-Nʹ-nitro-N-nitrosoguanidine in Escherichia colik-12.. Biochemical and Biophysical Research Communications 18:788–795
AlaeddinogluN. G.1976Genetical analysis of variation in the ability to utilize sucrose in strains of Escherichia coli. Ph.D. thesis Reading University;
BaronL. S.,
CareyW. F.,
SpilmanW. M.1959; Characterization of a high frequency of recombination (Hfr) strain of Salmonella typhosa compatible with Salmonella, Shigella and Escherichia species.. Proceedings of the National Academy of Sciences of the United States of America 45:976–983
BloomF. R.,
McFallE.,
YoungM. C.,
CarothersA. M.1975; Positive control in the d-serine deaminase system of Escherichia colik-12.. Journal of Bacteriology 121:1092–1101
BukhariA. I.,
TaylorA. L.1971; Genetic analysis of diaminopimelic acid- and lysine-requiring mutants of Escherichia coli. Journal of Bacteriology 105:844–854
Curtiss IIIR.,
CharamellaL. J.,
BergC. M.,
HarrisP. E.1965; Kinetic and genetic analyses of d-cycloserine inhibition and resistance in Escherichia coli. Journal of Bacteriology 90:1238–1250
HuangM.,
PittardJ.1967; Genetic analysis of mutant strains of Escherichia coli requiring p-aminobenzoic acid for growth.. Journal of Bacteriology 93:1938–1942
KornbergH. L.,
Jones-MortimerM. C.1977; The phosphotransferase system as a site of cellular control.. Symposia of the Society for General Microbiology 27:217–240
Le MinorL.,
CoynaultC.,
RohdeR.,
RoweB.,
AleksicS.1973; Localisation plasmidique du déterminant génétique du caractére atypique ‘Saccharose+’ des Salmonella. Annales de Microbiologie de l’Institut Pasteur 124B:295–306
LepesantJ.-A.,
KunstF.,
Lepesant-KejlarovaJ.,
DedonderR.1972; Chromosomal locations of mutations affecting sucrose metabolism in Bacillus subtilis Marburg.. Molecular and General Genetics 118:135–160
McFallE.1967b; Dominance studies with stable merodiploids in the d-serine deaminase system of Escherichia colik12. Journal of Bacteriology 94:1982–1988
McFallE.1973; Role of adenosine 3ʹ,5ʹ-cyclic monophosphate and its specific binding protein in the regulation of d-serine deaminase synthesis.. Journal of Bacteriology 113:781–785
OrnstonL. M.,
OrnstonM. K.,
ChouG.1969; Isolation of spontaneous mutant strains of Pseudomonas putida. Biochemical and Biophysical Research Communications 36:179–184
ØrskovI.,
ØrskovF.1973; Plasmid determined H2S character in Escherichia coli and its relation to plasmid-carried raffinose fermentation and tetracycline resistance characters.. Journal of General Microbiology 77:487–499
SaierM. H.,
StilesC. D.1975; Regulation of bacterial metabolism. In Molecular Dynamics in Biological Membranes pp. 99–105 New York, Heidelberg & Berlin: Springer Verlag;
VogelH. J.,
BonnerD. M.1956; A convenient growth medium for Escherichia coli and some other microorganisms (Medium E).. Microbial Genetics Bulletin 13:43–44
WangR. J.,
MorseH. G.,
MorseM. L.1969; Carbohydrate accumulation and metabolism in Escherichia coli: the close linkage and chromosomal location of ctr mutations.. Journal of Bacteriology 98:605–610
WangR. J.,
MorseH. G.,
MorseM. L.1970; Carbohydrate accumulation and metabolism in Escherichia coli: characteristics of the reversions of ctr mutations.. Journal of Bacteriology 104:1318–1324
WargelR. J.,
ShadurC. A.,
NeuhausF. C.1971; Mechanism of d-cycloserine action: transport mutants for d-alanine, d-cycloserine and glycine.. Journal of Bacteriology 105:1028–1035
WohlhieterJ. A.,
LazereJ. R.,
SnellingsN. J.,
JohnsonE. M.,
SynenkiR. M.,
BaronL. S.1974; Characterization of transmissible genetic elements from sucrose-fermenting Salmonella strains.. Journal of Bacteriology 122:401–406