@article{mbs:/content/journal/micro/10.1099/00221287-110-1-47, author = "Alaeddinoglu, N. Gürdal and Charles, H. P.", title = "Transfer of a Gene for Sucrose Utilization into Escherichia colik12, and Consequent Failure of Expression of Genes for d-Serine Utilization", journal= "Microbiology", year = "1979", volume = "110", number = "1", pages = "47-59", doi = "https://doi.org/10.1099/00221287-110-1-47", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-110-1-47", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: As the first stage in investigating the genetic basis of natural variation in Escherichia coli, the gene(s) conferring the ability to use sucrose as a carbon and energy source (given the symbol sac +) was transferred from a wild strain to k12, which does not use sucrose. The sac + region was transferred by two different methods. On both occasions it took a chromosomal location at minute 50·5 on the linkage map, between aroC and supN, in the region of the dsd genes, which confer the ability to use d-serine as a carbon and energy source. When the sac + region was present in the k12 chromosome the bacteria were unable to use d-serine as a carbon and energy source. In F'sac +/dsd + diploids, the dsd + genes were similarly not expressed. Strain k12(sac +) bacteria were sensitive to inhibition by d-serine; they mutated to d-serine resistance with much greater frequency than did a dsd mutant of k12. Such bacteria also mutated frequently to use raffinose. Strain k12(sac +) bacteria did not utilize sucrose when they carried a mutation affecting the phosphotransferase system.", }