1887

Abstract

Summary: Ferrichrome-promoted iron uptake in k12 is strictly dependent upon the gene product, a “minor” outer membrane protein. By selection for mutants of resistant to phages which require “major” outer membrane proteins as receptors, strains with pronounced protein deficiencies were constructed. Such strains were tested for anomalous behaviour of ferrichrome transport. No significant differences in iron uptake were detected in 12 strains with markedly reduced amounts of protein I. However, a reduction in the initial velocity (up to 40%) was observed in deficient in outer membrane protein II. This difference was only evident when cells were grown under iron-starvation conditions; it was abolished when cells were grown in rich medium. Kinetic parameters for ferrichrome transport were determined for maximum velocity but not for ; double reciprocal plots showed a biphasic nature, probably attributable to a limited number of outer membrane binding sites and to the multi-component nature of the ferrichrome-iron transport system.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-110-1-211
1979-01-01
2021-05-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/110/1/mic-110-1-211.html?itemId=/content/journal/micro/10.1099/00221287-110-1-211&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1959 Bacteriophages. New York: Interscience;
    [Google Scholar]
  2. Ames G. F., Spudich E. N., Nikaido H. 1974; Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations.. Journal of Bacteriology 117:406–416
    [Google Scholar]
  3. Braun V., Hantke K. 1977; Bacterial receptors for phages and colicins as constituents of specific transport systems. In Microbial Interactions (Receptors and Recognition, series B, vol. 3), pp. 100–137 Edited by Reissig J. L. London: Chapman and Hall;
    [Google Scholar]
  4. Braun V., Schaller K., Wabl M. R. 1974; Isolation, characterization and action of colicin M.. Antimicrobial Agents and Chemotherapy 5:520–533
    [Google Scholar]
  5. Braun V., Hancock R. E. W., Hantke K., Hartmann A. 1976; Functional organization of the outer membrane of Escherichia coli: phage and colicin receptors as components of iron uptake systems.. Journal of Supramolecular Structure 5:37–58
    [Google Scholar]
  6. Davis B. D., Mingioli E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12.. Journal of Bacteriology 60:17–28
    [Google Scholar]
  7. Haller I., Henning U. 1974; Cell envelope and shape of Escherichia coli k12. Crosslinking with dimethyl imidoesters of the whole cell wall.. Proceedings of the National Academy of Sciences of the United States of America 71:2018–2021
    [Google Scholar]
  8. Hancock R. E. W., Hantke K., Braun V. 1976; Iron transport in Escherichia coli k12: the involvement of the colicin B receptor and of a citrate-inducible protein.. Journal of Bacteriology 127:1370–1375
    [Google Scholar]
  9. Hantke K., Braun V. 1975 a; Membrane receptor dependent iron transport in Escherichia coli. FEBSLetters 49:301–305
    [Google Scholar]
  10. Hantke K., Braun V. 1975 b; A function common to iron-enterochelin transport and action of colicins B, I, V in Escherichia coli. FEBS Letters 59:277–281
    [Google Scholar]
  11. Henning U., Schmidmayer W., Hindennach I. 1977; Major proteins of the outer cell envelope membrane of Escherichia coli k12 : multiple species of protein I.. Molecular and General Genetics 154:293–298
    [Google Scholar]
  12. Hirota Y., Suzuki H., Nishimura Y., Yosuda S. 1977; On the process of cellular division in Escherichia coli: a mutant of Escherichia coli lacking a murein-lipoprotein.. Proceedings of the National Academy of Sciences of the United States of America 74:1417–1420
    [Google Scholar]
  13. Luckey M., Wayne R., Neilands J. B. 1975; In vitro competition between ferrichrome and phage for the outer membrane T5 receptor complex of Escherichia coli. Biochemical and Biophysical Research Communications 64:687–693
    [Google Scholar]
  14. Lugtenberg B., Meijers J., Peters R., Van der Hoek P., Van Alphen L. 1975; Electrophoretic resolution of the ‘major outer membrane protein’ of Escherichia coli k12 into four bands.. FEBS Letters 58:254–258
    [Google Scholar]
  15. Manning P. A., Pugsley A. P., Reeves P. 1977; Defective growth functions in mutants of Escherichia coli k12 lacking a major outer membrane protein.. Journal of Molecular Biology 116:285–300
    [Google Scholar]
  16. McIntosh M. A., Earhart C. F. 1977; Co-ordinate regulation by iron of the synthesis of phenolate compounds and three outer membrane proteins in Escherichia coli. Journal of Bacteriology 131:331–339
    [Google Scholar]
  17. Miller J. H. 1972 Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Nakae T., Nikaido H. 1975; Outer membrane as diffusion barrier in Salmonella typhimurium. Penetration of oligo- and polysaccharides into isolated outer membrane vesicles and cells with degraded peptidoglycan layer.. Journal of Biological Chemistry 250:7359–7365
    [Google Scholar]
  19. Nikaido H., Bavoil P., Hirota Y. 1977; Outer membranes of Gram-negative bacteria. XV. Transmembrane diffusion rates in Lipoproteindeficient mutants of Escherichia coli. Journal of Bacteriology 132:1045–1047
    [Google Scholar]
  20. Osborn M. J., Gander J. E., Parisi E., Carson J. 1972; Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.. Journal of Biological Chemistry 247:3962–3972
    [Google Scholar]
  21. Palva E. T., Randall L. L. 1978; Arrangement of protein I in Escherichia coli outer membrane : cross-linking study.. Journal of Bacteriology 133:279–286
    [Google Scholar]
  22. Reithmeier R. A. F., Bragg P. D. 1977; Crosslinking of the proteins in the outer membrane of Escherichia coli. Biochimica et biophysica acta 466:245–256
    [Google Scholar]
  23. Schweizer M., Henning U. 1977; Action of a major outer cell envelope membrane protein in conjugation of Escherichia coli k12.. Journal of Bacteriology 129:1651–1652
    [Google Scholar]
  24. Schweizer M., Hindennach I., Garten W., Henning U. 1978; Major proteins of the Escherichia coli outer cell envelope membrane. Interaction of protein II* with lipopolysaccharide.. European Journal of Biochemistry 82:211–217
    [Google Scholar]
  25. Van Alphen W., Lugtenberg B. 1977; Influence of osmolarity of the growth medium on the outer membrane protein pattern of Escherichia coli. Journal of Bacteriology 131:623–630
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-110-1-211
Loading
/content/journal/micro/10.1099/00221287-110-1-211
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error