RT Journal Article SR Electronic(1) A1 Pettipher, Graham L. A1 Latham, Malcolm J.YR 1979 T1 Characteristics of Enzymes Produced by Ruminococcus flavefaciens which Degrade Plant Cell Walls JF Microbiology, VO 110 IS 1 SP 21 OP 27 DO https://doi.org/10.1099/00221287-110-1-21 PB Microbiology Society, SN 1465-2080, AB Summary: Enzyme preparations active against crystalline cellulose, marble-milled filter paper, carboxymethyicellulose (CM-cellulose), hemicellulose and xylan were obtained from cultures of Ruminococcus flavefaciens. These preparations also contained swelling factor, pectin methylesterase, pectin lyase and low levels of aryl β-glucosidase and aryl β-xylosidase. CM-cellulase and xylanase activities were present in a high molecular weight complex, but substrate competition studies showed that different active sites were probably responsible for each activity. Analysis of products and viscosity changes during enzymic hydrolysis of CM-cellulose and xylan indicated that the most active enzymes were of the exo-1, 4-β-glycosidase type. A variety of reducing sugars were released from cell walls of Lolium perenne (perennial ryegrass) by enzyme preparations., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-110-1-21