Summary: Enzyme preparations active against crystalline cellulose, marble-milled filter paper, carboxymethyicellulose (CM-cellulose), hemicellulose and xylan were obtained from cultures of . These preparations also contained swelling factor, pectin methylesterase, pectin lyase and low levels of aryl β-glucosidase and aryl β-xylosidase. CM-cellulase and xylanase activities were present in a high molecular weight complex, but substrate competition studies showed that different active sites were probably responsible for each activity. Analysis of products and viscosity changes during enzymic hydrolysis of CM-cellulose and xylan indicated that the most active enzymes were of the exo-1, 4-β-glycosidase type. A variety of reducing sugars were released from cell walls of (perennial ryegrass) by enzyme preparations.


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