Summary: The T incompatibility group plasmid R394 can mobilize the chromosome of strain 5006. It transferred relatively large segments, corresponding to at least 20 min on the D plasmid chromosomal map of the organism. The frequency of recombination for a large number of selected markers was nearly constant at 5 × 10 per donor cell and it is concluded that mobilization takes place from a number of chromosomal sites. All recombinants were R and displayed all properties of the plasmid. By analysing crosses for co-inheritance frequencies of unselected markers, a number of chromosomal loci were assembled in linear array. Linkage between markers at the ends of this linkage group was established to markers at the respective termini of the existing D plasmid linkage group. This established a composite circular linkage map of genes of the strain 5006 chromosome.


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