1887

Abstract

Summary: The T incompatibility group plasmid R394 can mobilize the chromosome of strain 5006. It transferred relatively large segments, corresponding to at least 20 min on the D plasmid chromosomal map of the organism. The frequency of recombination for a large number of selected markers was nearly constant at 5 × 10 per donor cell and it is concluded that mobilization takes place from a number of chromosomal sites. All recombinants were R and displayed all properties of the plasmid. By analysing crosses for co-inheritance frequencies of unselected markers, a number of chromosomal loci were assembled in linear array. Linkage between markers at the ends of this linkage group was established to markers at the respective termini of the existing D plasmid linkage group. This established a composite circular linkage map of genes of the strain 5006 chromosome.

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/content/journal/micro/10.1099/00221287-110-1-171
1979-01-01
2021-07-31
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References

  1. Beringer J. E., Hoggan S. A., Johnston A. W. B. 1978; Linkage mapping in Rhizobium leguminosarum by means of R plasmid-mediated recombination.. Journal of General Microbiology 104:201–207
    [Google Scholar]
  2. Clowes R. C., Hayes W. 1968 Experiments in Microbial Genetics. Oxford and Edinburgh: Blackwell Scientific Publications;
    [Google Scholar]
  3. Coetzee J. N. 1974; High frequency transduction of kanamycin resistance in Proteus mirabilis. Journal of General Microbiology 84:285–296
    [Google Scholar]
  4. Coetzee J. N. 1975; Chromosome transfer in Proteus mirabilis mediated by a hybrid plasmid.. Journal of General Microbiology 86:133–146
    [Google Scholar]
  5. Coetzee J. N. 1978a; Extension of a chromosome linkage group of Proteus mirabilis. Journal of General Microbiology 107:155–163
    [Google Scholar]
  6. Coetzee J. N. 1978b; Mobilization of the Proteus mirabilis chromosome by R plasmid R772. Journal of General Microbiology 108:103–109
    [Google Scholar]
  7. Coetzee J. N., Datta N., Hedges R. W. 1972; R factors from Proteus rettgeri. Journal of General Microbiology 72:543–552
    [Google Scholar]
  8. Coetzee J. N., Datta N., Hedges R. W., Appelbaum P. C. 1973; Transduction of R factors in Proteus mirabilis and P. rettgeri. Journal of General Microbiology 76:355–368
    [Google Scholar]
  9. Haas D., Holloway B. W. 1976; R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Molecular and General Genetics 144:243–251
    [Google Scholar]
  10. Kondorosi A., Kiss G. B., Forrai T., Vincze E., Banfalvi Z. 1977; Circular linkage map of Rhizobium meliloti chromosome.. Nature, London 268:525–527
    [Google Scholar]
  11. Meade H. M., Signer E. R. 1977; Genetic mapping of Rhizobium meliloti. Proceedings of the National Academy of Sciences of the United States of America 74:2076–2078
    [Google Scholar]
  12. Riley M., Anilionis A. 1978; Evolution of the bacterial genome.. Annual Review of Microbiology 32:519–560
    [Google Scholar]
  13. Sanderson K. E. 1976; Genetic relatedness in the family Enterobacteriaceae.. Annual Review of Microbiology 30:327–349
    [Google Scholar]
  14. Towner K. J. 1978; Chromosome mapping in Acinetobacter calcoaceticus. Journal of General Microbiology 104:175–180
    [Google Scholar]
  15. Towner K. J., Vivian A. 1976; RP4 fertility variants in Acinetobacter calcoaceticus. Genetical Research 28:301–306
    [Google Scholar]
  16. Watson J. M., Holloway B. W. 1978; Chromosome mapping in Pseudomonas aeruginosa pat. Journal of Bacteriology 133:1113–1125
    [Google Scholar]
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